P0A5L5 (MURF_MYCBO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase
EC=6.3.2.10

Alternative name(s):
D-alanyl-D-alanine-adding enzyme
UDP-MurNAc-pentapeptide synthetase

Gene names

Name:	murF
Ordered Locus Names:	Mb2181c

Organism

Mycobacterium bovis [Complete proteome] [HAMAP]

Taxonomic identifier

1765 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	510 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysine + D-alanyl-D-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine.
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the MurCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase activity Inferred from electronic annotation. Source: EC UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 510	510	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase		PRO_0000101702
Regions
	Nucleotide binding	136 – 142	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	P0A5L5 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 7BE83EDD1BAA2EC4	FASTA	510	51,633
	10 20 30 40 50 60 MIELTVAQIA EIVGGAVADI SPQDAAHRRV TGTVEFDSRA IGPGGLFLAL PGARADGHDH 70 80 90 100 110 120 AASAVAAGAA VVLAARPVGV PAIVVPPVAA PNVLAGVLEH DNDGSGAAVL AALAKLATAV 130 140 150 160 170 180 AAQLVAGGLT IIGITGSSGK TSTKDLMAAV LAPLGEVVAP PGSFNNELGH PWTVLRATRR 190 200 210 220 230 240 TDYLILEMAA RHHGNIAALA EIAPPSIGVV LNVGTAHLGE FGSREVIAQT KAELPQAVPH 250 260 270 280 290 300 SGAVVLNADD PAVAAMAKLT AARVVRVSRD NTGDVWAGPV SLDELARPRF TLHAHDAQAE 310 320 330 340 350 360 VRLGVCGDHQ VTNALCAAAV ALECGASVEQ VAAALTAAPP VSRHRMQVTT RGDGVTVIDD 370 380 390 400 410 420 AYNANPDSMR AGLQALAWIA HQPEATRRSW AVLGEMAELG EDAIAEHDRI GRLAVRLDVS 430 440 450 460 470 480 RLVVVGTGRS ISAMHHGAVL EGAWGSGEAT ADHGADRTAV NVADGDAALA LLRAELRPGD 490 500 510 VVLVKASNAA GLGAVADALV ADDTCGSVRP

References

[1]

"The complete genome sequence of Mycobacterium bovis." Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M., Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B., Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J. , Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G. Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003) [PubMed: 12788972] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-935 / AF2122/97.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX248341 Genomic DNA. Translation: CAD97034.1.
RefSeq	NP_855830.1. NC_002945.3.
3D structure databases
ProteinModelPortal	P0A5L5.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000017587; EBMYCP00000017422; EBMYCG00000017582.
GeneID	1091261.
GenomeReviews	Gene locus Mb2181c in contig BX248333_GR.
KEGG	mbo:Mb2181c.
PATRIC	18006502. VBIMycBov88188_2397.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000015779.
HOGENOM	HBG617121.
OMA	YAVIEMG.
ProtClustDB	CLSK791663.
Enzyme and pathway databases
BioCyc	MBOV233413:MB2181C-MONOMER.
Family and domain databases
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR005863. UDP-N-AcMur-pentapeptide_synth. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit.
KO	K01929.
PANTHER	PTHR23135:SF3. MurF. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
SUPFAM	SSF53244. Mur_ligase_C. 1 hit. SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMs	TIGR01143. MurF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURF_MYCBO

Accession

Primary (citable) accession number: P0A5L5
Secondary accession number(s): O06220

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	January 25, 2012
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program