P0A5L2 (MURA_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylglucosamine 1-carboxyvinyltransferase
EC=2.5.1.7

Alternative name(s):
Enoylpyruvate transferase
UDP-N-acetylglucosamine enolpyruvyl transferase
Short name=EPT

Gene names

Name:	murA
Synonyms:	murZ
Ordered Locus Names:	Rv1315, MT1355
ORF Names:	MTCY149.01, MTCY373.35

Organism

Mycobacterium tuberculosis [Reference proteome] [HAMAP]

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	418 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cell wall formation. Adds enolpyruvyl to UDP-N-acetylglucosamine By similarity. HAMAP-Rule MF_00111
Catalytic activity	Phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine. HAMAP-Rule MF_00111
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00111
Subcellular location	Cytoplasm Probable HAMAP-Rule MF_00111.
Sequence similarities	Belongs to the EPSP synthase family. MurA subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Molecular function	Transferase
PTM	Isopeptide bond Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	UDP-N-acetylgalactosamine biosynthetic process Inferred from electronic annotation. Source: InterPro cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW growth Inferred from mutant phenotype PubMed 12657046. Source: MTBBASE peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 418	418	UDP-N-acetylglucosamine 1-carboxyvinyltransferase HAMAP-Rule MF_00111		PRO_0000178890
Sites
	Active site	117	1	Proton donor By similarity
Amino acid modifications
	Cross-link	81		Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup) HAMAP-Rule MF_00111

Sequences

	Sequence		Length	Mass (Da)
	P0A5L2 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 3F9E9AF04C4C36F3	FASTA	418	44,064
	10 20 30 40 50 60 MAERFVVTGG NRLSGEVAVG GAKNSVLKLM AATLLAEGTS TITNCPDILD VPLMAEVLRG 70 80 90 100 110 120 LGATVELDGD VARITAPDEP KYDADFAAVR QFRASVCVLG PLVGRCKRAR VALPGGDAIG 130 140 150 160 170 180 SRPLDMHQAG LRQLGAHCNI EHGCVVARAE TLRGAEIQLE FPSVGATENI LMAAVVAEGV 190 200 210 220 230 240 TTIHNAAREP DVVDLCTMLN QMGAQVEGAG SPTMTITGVP RLHPTEHRVI GDRIVAATWG 250 260 270 280 290 300 IAAAMTRGDI SVAGVDPAHL QLVLHKLHDA GATVTQTDAS FRVTQYERPK AVNVATLPFP 310 320 330 340 350 360 GFPTDLQPMA IALASIADGT SMITENVFEA RFRFVEEMIR LGADARTDGH HAVVRGLPQL 370 380 390 400 410 SSAPVWCSDI RAGAGLVLAG LVADGDTEVH DVFHIDRGYP LFVENLVSLG AEIERVCC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Kempsell K.E., Kelly V.A., Hutchinson S.E., Duncan K. Submitted (MAR-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[2]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[3]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
[4]	"A novel method for the isolation of mycobacterial DNA." Gonzalez-y-Merchand J.A., Estrada-Garcia I., Colston M.J., Cox R.A. FEMS Microbiol. Lett. 135:71-77(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 306-418. Strain: ATCC 25618 / H37Rv.
[5]	"Prokayrotic ubiquitin-like protein (Pup) proteome of Mycobacterium tuberculosis." Festa R.A., McAllister F., Pearce M.J., Mintseris J., Burns K.E., Gygi S.P., Darwin K.H. PLoS ONE 5:E8589-E8589(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PUPYLATION AT LYS-81, IDENTIFICATION BY MASS SPECTROMETRY. Strain: ATCC 25618 / H37Rv.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X96711 Genomic DNA. Translation: CAA65472.1. BX842576 Genomic DNA. Translation: CAA97748.1. AL123456 Genomic DNA. Translation: CCP44072.1. AE000516 Genomic DNA. Translation: AAK45617.1. X87944 Genomic DNA. Translation: CAA61196.1.
PIR	G70775.
RefSeq	NP_215831.1. NC_000962.3. NP_335803.1. NC_002755.2. YP_006514692.1. NC_018143.1.
3D structure databases
ProteinModelPortal	P0A5L2.
SMR	P0A5L2. Positions 3-416.
ModBase	Search...
Protein-protein interaction databases
STRING	83332.Rv1315.
Proteomic databases
PRIDE	P0A5L2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAK45617; AAK45617; MT1355.
GeneID	13319897. 886921. 924705.
KEGG	mtc:MT1355. mtu:Rv1315. mtv:RVBD_1315.
PATRIC	18124744. VBIMycTub22151_1490.
Organism-specific databases
TubercuList	Rv1315.
Phylogenomic databases
eggNOG	COG0766.
HOGENOM	HOG000075602.
KO	K00790.
OMA	GTWAYAA.
ProtClustDB	PRK09369.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-15222.
UniPathway	UPA00219.
Family and domain databases
Gene3D	3.65.10.10. 2 hits.
HAMAP	MF_00111. MurA.
InterPro	IPR001986. Enolpyruvate_Tfrase_dom. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. IPR005750. UDP_GlcNAc_COvinyl_MurA. [Graphical view]
PANTHER	PTHR21090:SF4. PTHR21090:SF4. 1 hit.
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
SUPFAM	SSF55205. RNA3'_cycl/enolpyr_transf_A/B. 1 hit.
TIGRFAMs	TIGR01072. murA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURA_MYCTU

Accession

Primary (citable) accession number: P0A5L2
Secondary accession number(s): L0T6H7, Q10604

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	May 1, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program