P0A5K8 (MSCL_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Large-conductance mechanosensitive channel

Gene names

Name:	mscL
Ordered Locus Names:	Rv0985c, MT1013
ORF Names:	MTV044.13c

Organism

Mycobacterium tuberculosis

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	151 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell By similarity. HAMAP MF_00115
Subunit structure	Homopentamer By similarity. HAMAP MF_00115
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity HAMAP MF_00115.
Sequence similarities	Belongs to the MscL family.

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Ionic channel
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein homooligomerization Inferred from physical interaction. Source: MTBBASE
Cellular component	integral to plasma membrane Inferred from direct assay. Source: MTBBASE
Molecular function	ion channel activity Inferred from electronic annotation. Source: UniProtKB-KW mechanically gated channel activity Inferred from direct assay. Source: MTBBASE
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 151	151	Large-conductance mechanosensitive channel HAMAP MF_00115		PRO_0000192452
Regions
	Topological domain	1 – 14	14	Cytoplasmic By similarity
	Transmembrane	15 – 43	29	Helical; By similarity
	Topological domain	44 – 68	25	Extracellular By similarity
	Transmembrane	69 – 90	22	Helical; By similarity
	Topological domain	91 – 151	61	Cytoplasmic By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A5K8 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: D682D3F2C1651E9C	FASTA	151	16,023
	10 20 30 40 50 60 MLKGFKEFLA RGNIVDLAVA VVIGTAFTAL VTKFTDSIIT PLINRIGVNA QSDVGILRIG 70 80 90 100 110 120 IGGGQTIDLN VLLSAAINFF LIAFAVYFLV VLPYNTLRKK GEVEQPGDTQ VVLLTEIRDL 130 140 150 LAQTNGDSPG RHGGRGTPSP TDGPRASTES Q

References

[1]

"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.

[2]

"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX842575 Genomic DNA. Translation: CAA17584.1. AE000516 Genomic DNA. Translation: AAK45261.1.
PIR	E70821.
RefSeq	NP_215500.1. NC_000962.2. NP_335447.1. NC_002755.2.
3D structure databases
ProteinModelPortal	P0A5K8.
SMR	P0A5K8. Positions 1-125.
ModBase	Search...
Protein family/group databases
TCDB	1.A.22.1.2. large conductance mechanosensitive ion channel (MscL) family.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000003219; EBMYCP00000003219; EBMYCG00000003217. EBMYCT00000070399; EBMYCP00000068458; EBMYCG00000070394.
GeneID	885368. 925186.
GenomeReviews	Gene locus MT1013 in contig AE000516_GR. Gene locus Rv0985c in contig AL123456_GR.
KEGG	mtc:MT1013. mtu:Rv0985c.
PATRIC	18123972. VBIMycTub22151_1109.
TIGR	MT1013.
Organism-specific databases
TubercuList	Rv0985c.
Phylogenomic databases
GeneTree	EBGT00050000017393.
HOGENOM	HBG727604.
OMA	VIDFVIV.
PhylomeDB	P0A5K8.
ProtClustDB	PRK00567.
Family and domain databases
HAMAP	MF_00115. MscL. [Tree]
InterPro	IPR019823. Mechanosensitive_channel_CS. IPR001185. MS_channel. [Graphical view]
KO	K03282.
Pfam	PF01741. MscL. 1 hit. [Graphical view]
PRINTS	PR01264. MECHCHANNEL.
SUPFAM	SSF81330. MS_channel. 1 hit.
TIGRFAMs	TIGR00220. MscL. 1 hit.
PROSITE	PS01327. MSCL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MSCL_MYCTU

Accession

Primary (citable) accession number: P0A5K8
Secondary accession number(s): O53898

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	January 25, 2012
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program