P0A5K8 (MSCL_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Large-conductance mechanosensitive channel

Gene names

Name:	mscL
Ordered Locus Names:	Rv0985c, MT1013
ORF Names:	MTV044.13c

Organism

Mycobacterium tuberculosis [Reference proteome] [HAMAP]

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	151 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell By similarity. HAMAP-Rule MF_00115
Subunit structure	Homopentamer By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity HAMAP-Rule MF_00115.
Sequence similarities	Belongs to the MscL family.

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Ion channel
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein homooligomerization Inferred from physical interaction PubMed 9856938. Source: MTBBASE
Cellular_component	integral to plasma membrane Inferred from direct assay PubMed 15952732. Source: MTBBASE
Molecular_function	ion channel activity Inferred from electronic annotation. Source: InterPro mechanically gated channel activity Inferred from direct assay PubMed 10846181. Source: MTBBASE
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 151	151	Large-conductance mechanosensitive channel HAMAP-Rule MF_00115		PRO_0000192452
Regions
	Topological domain	1 – 14	14	Cytoplasmic By similarity
	Transmembrane	15 – 43	29	Helical; By similarity
	Topological domain	44 – 68	25	Extracellular By similarity
	Transmembrane	69 – 90	22	Helical; By similarity
	Topological domain	91 – 151	61	Cytoplasmic By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A5K8 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: D682D3F2C1651E9C	FASTA	151	16,023
	10 20 30 40 50 60 MLKGFKEFLA RGNIVDLAVA VVIGTAFTAL VTKFTDSIIT PLINRIGVNA QSDVGILRIG 70 80 90 100 110 120 IGGGQTIDLN VLLSAAINFF LIAFAVYFLV VLPYNTLRKK GEVEQPGDTQ VVLLTEIRDL 130 140 150 LAQTNGDSPG RHGGRGTPSP TDGPRASTES Q

References

[1]

"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.

[2]

"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX842575 Genomic DNA. Translation: CAA17584.1. AE000516 Genomic DNA. Translation: AAK45261.1. AL123456 Genomic DNA. Translation: CCP43735.1.
PIR	E70821.
RefSeq	NP_215500.1. NC_000962.3. NP_335447.1. NC_002755.2. YP_006514345.1. NC_018143.1.
3D structure databases
ProteinModelPortal	P0A5K8.
SMR	P0A5K8. Positions 1-125.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-13322N.
STRING	83332.Rv0985c.
Protein family/group databases
TCDB	1.A.22.1.2. large conductance mechanosensitive ion channel (MscL) family.
Proteomic databases
PRIDE	P0A5K8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAK45261; AAK45261; MT1013.
GeneID	13319545. 885368. 925186.
KEGG	mtc:MT1013. mtu:Rv0985c. mtv:RVBD_0985c.
PATRIC	18123972. VBIMycTub22151_1109.
Organism-specific databases
TubercuList	Rv0985c.
Phylogenomic databases
eggNOG	COG1970.
HOGENOM	HOG000226782.
KO	K03282.
OMA	YGSFIQT.
ProtClustDB	PRK00567.
Family and domain databases
HAMAP	MF_00115. MscL.
InterPro	IPR019823. Mechanosensitive_channel_CS. IPR001185. MS_channel. [Graphical view]
Pfam	PF01741. MscL. 1 hit. [Graphical view]
PRINTS	PR01264. MECHCHANNEL.
SUPFAM	SSF81330. MS_channel. 1 hit.
TIGRFAMs	TIGR00220. mscL. 1 hit.
PROSITE	PS01327. MSCL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MSCL_MYCTU

Accession

Primary (citable) accession number: P0A5K8
Secondary accession number(s): L0T5D5, O53898

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	May 1, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program