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P0A5J4 (MASZ_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Malate synthase G
EC=2.3.3.9
Gene names
Name:glcB
Ordered Locus Names:Rv1837c, MT1885
ORF Names:MTCY1A11.06
OrganismMycobacterium tuberculosis
Taxonomic identifier1773 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length741 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA. HAMAP MF_00641

Cofactor

Magnesium.

Pathway

Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2. HAMAP MF_00641

Subunit structure

Monomer By similarity. HAMAP MF_00641

Subcellular location

Cytoplasm By similarity HAMAP MF_00641.

Sequence similarities

Belongs to the malate synthase family. GlcB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed HAMAP MF_00641
Chain2 – 741740Malate synthase G HAMAP MF_00641
PRO_0000166889

Sites

Active site3391Proton acceptor
Active site6331Proton donor

Secondary structure

1 ................................................................................................................. 741
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P0A5J4 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: A92F54E0FE8B7C64

FASTA74180,403
        10         20         30         40         50         60 
MTDRVSVGNL RIARVLYDFV NNEALPGTDI DPDSFWAGVD KVVADLTPQN QALLNARDEL 

        70         80         90        100        110        120 
QAQIDKWHRR RVIEPIDMDA YRQFLTEIGY LLPEPDDFTI TTSGVDAEIT TTAGPQLVVP 

       130        140        150        160        170        180 
VLNARFALNA ANARWGSLYD ALYGTDVIPE TDGAEKGPTY NKVRGDKVIA YARKFLDDSV 

       190        200        210        220        230        240 
PLSSGSFGDA TGFTVQDGQL VVALPDKSTG LANPGQFAGY TGAAESPTSV LLINHGLHIE 

       250        260        270        280        290        300 
ILIDPESQVG TTDRAGVKDV ILESAITTIM DFEDSVAAVD AADKVLGYRN WLGLNKGDLA 

       310        320        330        340        350        360 
AAVDKDGTAF LRVLNRDRNY TAPGGGQFTL PGRSLMFVRN VGHLMTNDAI VDTDGSEVFE 

       370        380        390        400        410        420 
GIMDALFTGL IAIHGLKASD VNGPLINSRT GSIYIVKPKM HGPAEVAFTC ELFSRVEDVL 

       430        440        450        460        470        480 
GLPQNTMKIG IMDEERRTTV NLKACIKAAA DRVVFINTGF LDRTGDEIHT SMEAGPMVRK 

       490        500        510        520        530        540 
GTMKSQPWIL AYEDHNVDAG LAAGFSGRAQ VGKGMWTMTE LMADMVETKI AQPRAGASTA 

       550        560        570        580        590        600 
WVPSPTAATL HALHYHQVDV AAVQQGLAGK RRATIEQLLT IPLAKELAWA PDEIREEVDN 

       610        620        630        640        650        660 
NCQSILGYVV RWVDQGVGCS KVPDIHDVAL MEDRATLRIS SQLLANWLRH GVITSADVRA 

       670        680        690        700        710        720 
SLERMAPLVD RQNAGDVAYR PMAPNFDDSI AFLAAQELIL SGAQQPNGYT EPILHRRRRE 

       730        740 
FKARAAEKPA PSDRAGDDAA R

« Hide

References

« Hide 'large scale' references
[1]"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence."
Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. expand/collapse author list , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G.
Nature 393:537-544(1998) [PubMed: 9634230] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 25618 / H37Rv.
[2]"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains."
Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. expand/collapse author list , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.
J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CDC 1551 / Oshkosh.
[3]"Biochemical and structural studies of malate synthase from Mycobacterium tuberculosis."
Smith C.V., Huang C.C., Miczak A., Russell D.G., Sacchettini J.C., Honer zu Bentrup K.
J. Biol. Chem. 278:1735-1743(2003) [PubMed: 12393860] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 1-727, CHARACTERIZATION.
Strain: CDC 1551 / Oshkosh.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BX842578 Genomic DNA. Translation: CAB01465.1.
AE000516 Genomic DNA. Translation: AAK46156.1.
PIRF70722.
RefSeqNP_216353.1. NC_000962.2.
NP_336342.1. NC_002755.2.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1N8IX-ray2.10A1-741[»]
1N8WX-ray2.70A/B1-741[»]
2GQ3X-ray2.30A/B1-726[»]
ProteinModelPortalP0A5J4.
SMRP0A5J4. Positions 5-727.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBMYCT00000001528; EBMYCP00000001528; EBMYCG00000001528.
EBMYCT00000068990; EBMYCP00000067049; EBMYCG00000068985.
GeneID885713.
923734.
GenomeReviewsGene locus MT1885 in contig AE000516_GR.
Gene locus Rv1837c in contig AL123456_GR.
KEGGmtc:MT1885.
mtu:Rv1837c.
PATRIC18125911. VBIMycTub22151_2068.
TIGRMT1885.

Organism-specific databases

TubercuListRv1837c.

Phylogenomic databases

GeneTreeEBGT00070000032326.
HOGENOMHBG350005.
OMAPKMHGPD.
PhylomeDBP0A5J4.
ProtClustDBPRK02999.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-11953.

Family and domain databases

HAMAPMF_00641. Malate_synth_G.
[Tree]
InterProIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
Gene3DG3DSA:2.170.170.11. Malate_synth_G_beta_sub_dom. 2 hits.
KOK01638.
PANTHERPTHR21631:SF1. Malate_synthase. 1 hit.
PfamPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMSSF51645. Malat_synth_like. 1 hit.
TIGRFAMsTIGR01345. Malate_syn_G. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMASZ_MYCTU
AccessionPrimary (citable) accession number: P0A5J4
Secondary accession number(s): Q50596
Entry history
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: January 23, 2007
Last modified: January 25, 2012
This is version 54 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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