P0A5J3 (AMPM_MYCBO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methionine aminopeptidase
Short name=MAP
EC=3.4.11.18

Alternative name(s):
Peptidase M

Gene names

Name:	map
Synonyms:	mapB
Ordered Locus Names:	Mb2886c

Organism

Mycobacterium bovis [Complete proteome] [HAMAP]

Taxonomic identifier

1765 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	285 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Removes the amino-terminal methionine from nascent proteins By similarity.
Catalytic activity	Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.
Cofactor	Binds 2 cobalt ions per subunit. The true nature of the physiological cofactor is under debate. The enzyme is also active with zinc, manganese or divalent iron ions By similarity.
Sequence similarities	Belongs to the peptidase M24A family.

Ontologies

Keywords
Ligand	Cobalt Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular process Inferred from electronic annotation. Source: InterPro proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW metalloexopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 285	285	Methionine aminopeptidase		PRO_0000148949
Sites
	Metal binding	131	1	Cobalt 1 By similarity
	Metal binding	142	1	Cobalt 1 By similarity
	Metal binding	142	1	Cobalt 2 By similarity
	Metal binding	205	1	Cobalt 2 By similarity
	Metal binding	238	1	Cobalt 2 By similarity
	Metal binding	269	1	Cobalt 1 By similarity
	Metal binding	269	1	Cobalt 2 By similarity
	Binding site	114	1	Substrate By similarity
	Binding site	212	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A5J3 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: E69831250E6C6130	FASTA	285	30,891
	10 20 30 40 50 60 MPSRTALSPG VLSPTRPVPN WIARPEYVGK PAAQEGSEPW VQTPEVIEKM RVAGRIAAGA 70 80 90 100 110 120 LAEAGKAVAP GVTTDELDRI AHEYLVDNGA YPSTLGYKGF PKSCCTSLNE VICHGIPDST 130 140 150 160 170 180 VITDGDIVNI DVTAYIGGVH GDTNATFPAG DVADEHRLLV DRTREATMRA INTVKPGRAL 190 200 210 220 230 240 SVIGRVIESY ANRFGYNVVR DFTGHGIGTT FHNGLVVLHY DQPAVETIMQ PGMTFTIEPM 250 260 270 280 INLGALDYEI WDDGWTVVTK DRKWTAQFEH TLLVTDTGVE ILTCL

References

[1]

"The complete genome sequence of Mycobacterium bovis." Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M., Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B., Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J. , Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G. Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003) [PubMed: 12788972] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-935 / AF2122/97.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX248344 Genomic DNA. Translation: CAD96573.1.
RefSeq	NP_856531.1. NC_002945.3.
3D structure databases
ProteinModelPortal	P0A5J3.
SMR	P0A5J3. Positions 4-285.
ModBase	Search...
Protein family/group databases
MEROPS	M24.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000017725; EBMYCP00000017560; EBMYCG00000017720.
GeneID	1092223.
GenomeReviews	Gene locus Mb2886c in contig BX248333_GR.
KEGG	mbo:Mb2886c.
PATRIC	18008068. VBIMycBov88188_3166.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000016752.
HOGENOM	HBG299384.
OMA	IEPMISE.
ProtClustDB	PRK12896.
Enzyme and pathway databases
BioCyc	MBOV233413:MB2886C-MONOMER.
Family and domain databases
InterPro	IPR001714. Pept_M24_MAP. IPR000994. Pept_M24_structural-domain. IPR002467. Pept_M24A_MAP1. [Graphical view]
Gene3D	G3DSA:3.90.230.10. Peptidase_M24_cat_core. 1 hit.
KO	K01265.
Pfam	PF00557. Peptidase_M24. 1 hit. [Graphical view]
PRINTS	PR00599. MAPEPTIDASE.
SUPFAM	SSF55920. Peptidase_M24_cat_core. 1 hit.
TIGRFAMs	TIGR00500. Met_pdase_I. 1 hit.
PROSITE	PS00680. MAP_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPM_MYCBO

Accession

Primary (citable) accession number: P0A5J3
Secondary accession number(s): O33343

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program