P0A5I8 (LPRG_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lipoprotein LprG

Alternative name(s):
27 kDa lipoprotein
Antigen P27

Gene names

Name:	lprG
Synonyms:	lpp-27
Ordered Locus Names:	Rv1411c, MT1455
ORF Names:	MTCY21B4.28c

Organism

Mycobacterium tuberculosis [Reference proteome] [HAMAP]

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	236 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	TLR2 agonist that can inhibit primary human macrophage MHC-II Ag processing. Required for Rv1410c (P55) activity. Ref.3 Ref.4
Subcellular location	Cell membrane; Lipid-anchor.
Disruption phenotype	Cells that lack the lprG-Rv1410c operon display abnormal colony morphology and are defective for sliding motility. Ref.4
Sequence similarities	Belongs to the LppX/LprAFG lipoprotein family.
Sequence caution	The sequence AAK45720.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Virulence
Cellular component	Cell membrane Membrane
Domain	Signal
PTM	Lipoprotein Palmitate
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	pathogenesis Inferred from mutant phenotype PubMed 14998516. Source: MTBBASE
Cellular_component	cell wall Inferred from direct assay PubMed 20825248. Source: MTBBASE plasma membrane Inferred from direct assay PubMed 14532352PubMed 15525680. Source: MTBBASE
Molecular_function	glycolipid binding Inferred from direct assay PubMed 20694006. Source: MTBBASE
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 26

26

Probable

Chain

27 – 236

210

Lipoprotein LprG

PRO_0000018146

Amino acid modifications

Lipidation

27

1

N-palmitoyl cysteine Probable

Lipidation

27

1

S-diacylglycerol cysteine Probable

Secondary structure

1

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236

Helix Strand Turn

Details...

Helix

41 – 53

13

Beta strand

57 – 66

10

Beta strand

73 – 81

9

Turn

82 – 85

4

Beta strand

86 – 95

10

Beta strand

98 – 107

10

Beta strand

110 – 116

7

Beta strand

119 – 125

7

Helix

126 – 128

3

Helix

132 – 135

4

Turn

138 – 140

3

Helix

142 – 147

6

Beta strand

149 – 160

12

Beta strand

163 – 172

10

Helix

174 – 180

7

Helix

182 – 184

3

Beta strand

190 – 199

10

Beta strand

204 – 212

9

Beta strand

215 – 223

9

Sequences

	Sequence		Length	Mass (Da)
	P0A5I8 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 2591DAE6D2E2DC22	FASTA	236	24,548
	10 20 30 40 50 60 MRTPRRHCRR IAVLAAVSIA ATVVAGCSSG SKPSGGPLPD AKPLVEEATA QTKALKSAHM 70 80 90 100 110 120 VLTVNGKIPG LSLKTLSGDL TTNPTAATGN VKLTLGGSDI DADFVVFDGI LYATLTPNQW 130 140 150 160 170 180 SDFGPAADIY DPAQVLNPDT GLANVLANFA DAKAEGRDTI NGQNTIRISG KVSAQAVNQI 190 200 210 220 230 APPFNATQPV PATVWIQETG DHQLAQAQLD RGSGNSVQMT LSKWGEKVQV TKPPVS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[2]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
[3]	"Mycobacterium tuberculosis LprG (Rv1411c): a novel TLR-2 ligand that inhibits human macrophage class II MHC antigen processing." Gehring A.J., Dobos K.M., Belisle J.T., Harding C.V., Boom W.H. J. Immunol. 173:2660-2668(2004) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION. Strain: ATCC 25618 / H37Rv.
[4]	"Function of a mycobacterial major facilitator superfamily pump requires a membrane-associated lipoprotein." Farrow M.F., Rubin E.J. J. Bacteriol. 190:1783-1791(2008) [PubMed] [Europe PMC] [Abstract] Cited for: DISRUPTION PHENOTYPE, FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

BX842576 Genomic DNA. Translation: CAB02197.1.
AE000516 Genomic DNA. Translation: AAK45720.1. Different initiation.
AL123456 Genomic DNA. Translation: CCP44170.1.

PIR

H70901.

RefSeq

NP_215927.1. NC_000962.3.
NP_335906.1. NC_002755.2.
YP_006514791.1. NC_018143.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3MH8	X-ray	2.00	A/B	36-231	[»]
3MH9	X-ray	1.79	A/C	28-236	[»]
3MHA	X-ray	1.85	A/B	36-231	[»]

ProteinModelPortal

P0A5I8.

SMR

P0A5I8. Positions 34-236.

ModBase

Search...

Protein-protein interaction databases

STRING

83332.Rv1411c.

Proteomic databases

PaxDb

P0A5I8.

PRIDE

P0A5I8.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAK45720; AAK45720; MT1455.

GeneID

13320000.
886700.
924517.

KEGG

mtc:MT1455.
mtu:Rv1411c.
mtv:RVBD_1411c.

PATRIC

18124968. VBIMycTub22151_1598.

Organism-specific databases

TubercuList

Rv1411c.

Phylogenomic databases

eggNOG

NOG10313.

HOGENOM

HOG000046582.

KO

K14954.

OMA

PGNSVTM.

ProtClustDB

CLSK791146.

Family and domain databases

InterPro

IPR009830. DUF1396.
[Graphical view]

Pfam

PF07161. DUF1396. 1 hit.
[Graphical view]

ProDom

PD017293. DUF1396. 1 hit.
[Graphical view] [Entries sharing at least one domain]

PROSITE

PS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P0A5I8.

Entry information

Entry name

LPRG_MYCTU

Accession

Primary (citable) accession number: P0A5I8
Secondary accession number(s): L0T868, O32852, P71679

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	May 1, 2013
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program