P0A5I7 (BLAC_MYCBO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-lactamase
EC=3.5.2.6

Alternative name(s):
Penicillinase

Gene names

Name:	blaA
Synonyms:	blaC
Ordered Locus Names:	Mb2094c

Organism

Mycobacterium bovis [Complete proteome] [HAMAP]

Taxonomic identifier

1765 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	307 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Subcellular location	Cell membrane; Lipid-anchor Potential.
Sequence similarities	Belongs to the class-A beta-lactamase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Cellular component	Cell membrane Membrane
Domain	Signal
Molecular function	Hydrolase
PTM	Lipoprotein Palmitate
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	beta-lactam antibiotic catabolic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	beta-lactamase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 307	284	Beta-lactamase		PRO_0000017004
Regions
	Region	250 – 252	3	Substrate binding By similarity
Sites
	Active site	84	1	Acyl-ester intermediate By similarity
Amino acid modifications
	Lipidation	24	1	N-palmitoyl cysteine Potential
	Lipidation	24	1	S-diacylglycerol cysteine Potential

Sequences

	Sequence		Length	Mass (Da)
	P0A5I7 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 448CB2A0E05F4315	FASTA	307	32,568
	10 20 30 40 50 60 MRNRGFGRRE LLVAMAMLVS VTGCARHASG ARPASTTLPA GADLADRFAE LERRYDARLG 70 80 90 100 110 120 VYVPATGTTA AIEYRADERF AFCSTFKAPL VAAVLHQNPL THLDKLITYT SDDIRSISPV 130 140 150 160 170 180 AQQHVQTGMT IGQLCDAAIR YSDGTAANLL LADLGGPGGG TAAFTGYLRS LGDTVSRLDA 190 200 210 220 230 240 EEPELNRDPP GDERDTTTPH AIALVLQQLV LGNALPPDKR ALLTDWMARN TTGAKRIRAG 250 260 270 280 290 300 FPADWKVIDK TGTGDYGRAN DIAVVWSPTG VPYVVAVMSD RAGGGYDAEP REALLAEAAT CVAGVLA

References

[1]

"The complete genome sequence of Mycobacterium bovis." Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M., Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B., Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J. , Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G. Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003) [PubMed: 12788972] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-935 / AF2122/97.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX248341 Genomic DNA. Translation: CAD96947.1.
RefSeq	NP_855744.1. NC_002945.3.
3D structure databases
ProteinModelPortal	P0A5I7.
SMR	P0A5I7. Positions 43-307.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000015862; EBMYCP00000015697; EBMYCG00000015858.
GeneID	1090361.
GenomeReviews	Gene locus Mb2094c in contig BX248333_GR.
KEGG	mbo:Mb2094c.
PATRIC	18006312. VBIMycBov88188_2303.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000016522.
HOGENOM	HBG535713.
OMA	EYGRAND.
ProtClustDB	CLSK791603.
Enzyme and pathway databases
BioCyc	MBOV233413:MB2094C-MONOMER.
Family and domain databases
InterPro	IPR001466. Beta-lactam-related. IPR012338. Beta-lactam/transpept-like. IPR000871. Beta-lactam_class-A/D. IPR023650. Beta-lactam_class-A_AS. [Graphical view]
Gene3D	G3DSA:3.40.710.10. G3DSA:3.40.710.10. 1 hit.
KO	K01467.
Pfam	PF00144. Beta-lactamase. 1 hit. [Graphical view]
PRINTS	PR00118. BLACTAMASEA.
SUPFAM	SSF56601. PBP_transp_fold. 1 hit.
PROSITE	PS00146. BETA_LACTAMASE_A. 1 hit. PS51257. PROKAR_LIPOPROTEIN. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BLAC_MYCBO

Accession

Primary (citable) accession number: P0A5I7
Secondary accession number(s): Q10670

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	January 25, 2012
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program