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P0A5I3 (ISPH2_MYCBO) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 42. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
4-hydroxy-3-methylbut-2-enyl diphosphate reductase 2
EC=1.17.1.2
Gene names
Name:ispH2
Synonyms:lytB2
Ordered Locus Names:Mb3414c
OrganismMycobacterium bovis [Complete proteome] [HAMAP]
Taxonomic identifier1765 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length329 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) By similarity. HAMAP MF_00191

Catalytic activity

Isopentenyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191

Dimethylallyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191

Cofactor

Binds 1 3Fe-4S cluster By similarity. HAMAP MF_00191

Pathway

Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1. HAMAP MF_00191

Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6. HAMAP MF_00191

Sequence similarities

Belongs to the IspH family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 3293294-hydroxy-3-methylbut-2-enyl diphosphate reductase 2 HAMAP MF_00191
PRO_0000128837

Sequences

Sequence LengthMass (Da)Tools
P0A5I3 [UniParc].

Last modified March 15, 2005. Version 1.
Checksum: 00540C5C87B2AB6B

FASTA32934,666
        10         20         30         40         50         60 
MAEVFVGPVA QGYASGEVTV LLASPRSFCA GVERAIETVK RVLDVAEGPV YVRKQIVHNT 

        70         80         90        100        110        120 
VVVAELRDRG AVFVEDLDEI PDPPPPGAVV VFSAHGVSPA VRAGADERGL QVVDATCPLV 

       130        140        150        160        170        180 
AKVHAEAARF AARGDTVVFI GHAGHEETEG TLGVAPRSTL LVQTPADVAA LNLPEGTQLS 

       190        200        210        220        230        240 
YLTQTTLALD ETADVIDALR ARFPTLGQPP SEDICYATTN RQRALQSMVG ECDVVLVIGS 

       250        260        270        280        290        300 
CNSSNSRRLV ELAQRSGTPA YLIDGPDDIE PEWLSSVSTI GVTAGASAPP RLVGQVIDAL 

       310        320 
RGYASITVVE RSIATETVRF GLPKQVRAQ

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BX248345 Genomic DNA. Translation: CAD95586.1.
RefSeqNP_857055.1. NC_002945.3.

3D structure databases

ProteinModelPortalP0A5I3.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBMYCT00000016587; EBMYCP00000016422; EBMYCG00000016582.
GeneID1090957.
GenomeReviewsGene locus Mb3414c in contig BX248333_GR.
KEGGmbo:Mb3414c.
PATRIC18009244. VBIMycBov88188_3750.

Organism-specific databases

CMRSearch...

Phylogenomic databases

GeneTreeEBGT00050000016858.
HOGENOMHBG335228.
OMAHNQGKRY.
ProtClustDBCLSK799986.

Enzyme and pathway databases

BioCycMBOV233413:MB3414C-MONOMER.

Family and domain databases

HAMAPMF_00191. IspH.
[Tree]
InterProIPR003451. LytB.
[Graphical view]
KOK03527.
PfamPF02401. LYTB. 1 hit.
[Graphical view]
TIGRFAMsTIGR00216. IspH_lytB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameISPH2_MYCBO
AccessionPrimary (citable) accession number: P0A5I3
Secondary accession number(s): O50409
Entry history
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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