P0A5B1 (HIS2_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosyl-ATP pyrophosphatase
Short name=PRA-PH
EC=3.6.1.31

Gene names

Name:	hisE
Ordered Locus Names:	Rv2122c, MT2182
ORF Names:	MTCY261.18

Organism

Mycobacterium tuberculosis

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	93 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	1-(5-phosphoribosyl)-ATP + H2O = 1-(5-phosphoribosyl)-AMP + diphosphate. HAMAP MF_01020
Pathway	Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/9. HAMAP MF_01020
Subcellular location	Cytoplasm By similarity HAMAP MF_01020.
Sequence similarities	Belongs to the PRA-PH family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Histidine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	growth Inferred from mutant phenotype. Source: MTBBASE histidine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW response to host iron concentration Inferred from mutant phenotype. Source: MTBBASE
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from direct assay. Source: MTBBASE phosphoribosyl-ATP diphosphatase activity Inferred from electronic annotation. Source: EC protein homodimerization activity Inferred from physical interaction. Source: MTBBASE
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 93

93

Phosphoribosyl-ATP pyrophosphatase HAMAP MF_01020

PRO_0000136371

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

93

Helix Strand Turn

Details...

Helix

10 – 23

14

Helix

29 – 35

7

Helix

38 – 58

21

Helix

61 – 82

22

Helix

86 – 90

5

Sequences

	Sequence		Length	Mass (Da)
	P0A5B1 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: E4CA193791812D1D	FASTA	93	10,275
	10 20 30 40 50 60 MQQSLAVKTF EDLFAELGDR ARTRPADSTT VAALDGGVHA LGKKLLEEAG EVWLAAEHES 70 80 90 NDALAEEISQ LLYWTQVLMI SRGLSLDDVY RKL

References

[1]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[2]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

BX842578 Genomic DNA. Translation: CAE55456.1.
AE000516 Genomic DNA. Translation: AAK46465.1.

PIR

E70513.

RefSeq

NP_336651.1. NC_002755.2.
YP_177860.1. NC_000962.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1Y6X	X-ray	1.25	A	1-93	[»]
3C90	X-ray	1.79	A/B/C/X	2-93	[»]

ProteinModelPortal

P0A5B1.

SMR

P0A5B1. Positions 7-93.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBMYCT00000000925; EBMYCP00000000925; EBMYCG00000000925.
EBMYCT00000070909; EBMYCP00000068968; EBMYCG00000070904.

GeneID

888671.
924290.

GenomeReviews

Gene locus MT2182 in contig AE000516_GR.
Gene locus Rv2122c in contig AL123456_GR.

KEGG

mtc:MT2182.
mtu:Rv2122c.

PATRIC

18126554. VBIMycTub22151_2390.

TIGR

MT2182.

Organism-specific databases

TubercuList

Rv2122c.

Phylogenomic databases

GeneTree

EBGT00050000017721.

HOGENOM

HBG646527.

OMA

VWMAAEY.

PhylomeDB

P0A5B1.

ProtClustDB

PRK00400.

Family and domain databases

HAMAP

MF_01020. HisE.
[Tree]

InterPro

IPR008179. PRib-ATP_PPHydrolase.
IPR021130. PRib-ATP_PPHydrolase-like.
[Graphical view]

KO

K01523.

Pfam

PF01503. PRA-PH. 1 hit.
[Graphical view]

TIGRFAMs

TIGR03188. Histidine_hisI. 1 hit.

ProtoNet

Search...

Entry information

Entry name

HIS2_MYCTU

Accession

Primary (citable) accession number: P0A5B1
Secondary accession number(s): O33257

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	January 25, 2012
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program