P0A5A7 (PPOX_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protoporphyrinogen oxidase
Short name=PPO
EC=1.3.3.4

Gene names

Name:	hemY
Ordered Locus Names:	Rv2677c, MT2751
ORF Names:	MTV010.01c

Organism

Mycobacterium tuberculosis [Reference proteome] [HAMAP]

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	452 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX.
Catalytic activity	Protoporphyrinogen-IX + 3 O2 = protoporphyrin-IX + 3 H2O2.
Cofactor	Binds 1 FAD per dimer By similarity.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; protoporphyrin-IX from protoporphyrinogen-IX: step 1/1.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the protoporphyrinogen oxidase family.

Ontologies

Keywords
Biological process	Heme biosynthesis Porphyrin biosynthesis
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protoporphyrinogen IX biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	oxygen-dependent protoporphyrinogen oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 452	452	Protoporphyrinogen oxidase		PRO_0000135265
Regions
	Nucleotide binding	10 – 15	6	FAD Potential

Sequences

	Sequence		Length	Mass (Da)
	P0A5A7 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 6DFA1F57BEE2810F	FASTA	452	46,846
	10 20 30 40 50 60 MTPRSYCVVG GGISGLTSAY RLRQAVGDDA TITLFEPADR LGGVLRTEHI GGQPMDLGAE 70 80 90 100 110 120 AFVLRRPEMP ALLAELGLSD RQLASTGARP LIYSQQRLHP LPPQTVVGIP SSAGSMAGLV 130 140 150 160 170 180 DDATLARIDA EAARPFTWQV GSDPAVADLV ADRFGDQVVA RSVDPLLSGV YAGSAATIGL 190 200 210 220 230 240 RAAAPSVAAA LDRGATSVTD AVRQALPPGS GGPVFGALDG GYQVLLDGLV RRSRVHWVRA 250 260 270 280 290 300 RVVQLERGWV LRDETGGRWQ ADAVILAVPA PRLARLVDGI APRTHAAARQ IVSASSAVVA 310 320 330 340 350 360 LAVPGGTAFP HCSGVLVAGD ESPHAKAITL SSRKWGQRGD VALLRLSFGR FGDEPALTAS 370 380 390 400 410 420 DDQLLAWAAD DLVTVFGVAV DPVDVRVRRW IEAMPQYGPG HADVVAELRA GLPPTLAVAG 430 440 450 SYLDGIGVPA CVGAAGRAVT SVIEALDAQV AR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[2]	"Re-annotation of the genome sequence of Mycobacterium tuberculosis H37Rv." Camus J.-C., Pryor M.J., Medigue C., Cole S.T. Microbiology 148:2967-2973(2002) [PubMed] [Europe PMC] [Abstract] Cited for: SEQUENCE REVISION.
[3]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX842580 Genomic DNA. Translation: CAE55510.1. AE000516 Genomic DNA. Translation: AAK47066.1. AL123456 Genomic DNA. Translation: CCP45475.1.
RefSeq	NP_337252.1. NC_002755.2. YP_006516121.1. NC_018143.1. YP_177675.1. NC_000962.3.
3D structure databases
ProteinModelPortal	P0A5A7.
SMR	P0A5A7. Positions 8-443.
ModBase	Search...
Protein-protein interaction databases
STRING	83332.Rv2677c.
Proteomic databases
PRIDE	P0A5A7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAK47066; AAK47066; MT2751.
GeneID	13319404. 887711. 925547.
KEGG	mtc:MT2751. mtu:Rv2677c. mtv:RVBD_2677c.
PATRIC	18127788. VBIMycTub22151_2993.
Organism-specific databases
TubercuList	Rv2677c.
Phylogenomic databases
eggNOG	COG1232.
HOGENOM	HOG000269479.
KO	K00231.
OMA	SWPGKLR.
ProtClustDB	PRK11883.
Enzyme and pathway databases
UniPathway	UPA00251; UER00324.
Family and domain databases
InterPro	IPR002937. Amino_oxidase. IPR004572. Protoporphyrinogen_oxidase. [Graphical view]
Pfam	PF01593. Amino_oxidase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00562. proto_IX_ox. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PPOX_MYCTU

Accession

Primary (citable) accession number: P0A5A7
Secondary accession number(s): L0TAB5, O53230

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	May 1, 2013
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program