Reviewed, UniProtKB/Swiss-Prot P0A586 (G6PD2_MYCTU)

Names and origin

Protein names

Recommended name:
    Probable glucose-6-phosphate 1-dehydrogenase
      Short name=G6PD
    EC=1.1.1.49

Gene names

Name:	zwf2
Synonyms:	zwf
Ordered Locus Names:	Rv1121, MT1153
ORF Names:	MTCY22G8.10

Organism

Mycobacterium tuberculosis [Complete proteome] [HAMAP]

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	466 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	D-glucose 6-phosphate + NADP+ = D-glucono-1,5-lactone 6-phosphate + NADPH.
Pathway	Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.
Sequence similarities	Belongs to the glucose-6-phosphate dehydrogenase family.
Caution	M.tuberculosis has two genes for zwf. This one is highly divergent.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glucose metabolism
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro glucose-6-phosphate dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 466	466	Probable glucose-6-phosphate 1-dehydrogenase		PRO_0000068128
Sites
	Active site	233	1	Proton acceptor By similarity
	Binding site	18	1	NADP By similarity
	Binding site	48	1	NADP By similarity
	Binding site	171	1	Substrate By similarity
	Binding site	175	1	Substrate By similarity
	Binding site	324	1	Substrate By similarity
Experimental info
	Sequence conflict	317	1	A → S in AAK45410. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P0A586-1 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 536302013052A596	FASTA	466	52,173
	10 20 30 40 50 60 MVDGGGGASD LLVIFGITGD LARKMTFRAL YRLERHQLLD CPILGVASDD MSVGQLVKWA 70 80 90 100 110 120 RESIGRTEKI DDAVFDRLAG RLSYLHGDVT DSQLYDSLAE LIGSACRPLY YLEMPPALFA 130 140 150 160 170 180 PIVENLANVR LLERARVAVE KPFGHDLASA LELNARLRAV LGEDQILRVD HFLGKQPVVE 190 200 210 220 230 240 LEYLRFANQA LAELWDRNSI SEIHITMAED FGVEDRGKFY DAVGALRDVV QNHLLQVLAL 250 260 270 280 290 300 VTMEPPVGSS ADDLNDKKAE VFRAMAPLDP DRCVRGQYLG YTEVAGVASD SATETYVALR 310 320 330 340 350 360 TEIDNWRWAG VPIFVRAGKE LPAKVTEVRL FLRRVPALAF LPNRRPAEPN QIVLRIDPDP 370 380 390 400 410 420 GMRLQISAHT DDSWRDIHLD SSFAVDLGEP IRPYERLLYA GLVGDHQLFA REDSIEQTWR 430 440 450 460 IVQPLLDNPG EIHRYDRGSW GPEAAQSLLR GHRGWQSPWL PRGTDA

References

[1]

"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.

[2]

"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX842575 Genomic DNA. Translation: CAE55363.1. AE000516 Genomic DNA. Translation: AAK45410.1.
PIR	B70538.
RefSeq	NP_335596.1. YP_177789.1.
3D structure databases
HSSP	HSSP built from PDB template 1QKI based on UniProtKB P11413.
ModBase	Search...
Genome annotation databases
GeneID	885817. 924943.
GenomeReviews	Gene locus MT1153 in contig AE000516_GR. Gene locus Rv1121 in contig AL123456_GR.
KEGG	mtc:MT1153. mtu:Rv1121.
TIGR	MT1153.
Organism-specific databases
TubercuList	Rv1121.
Phylogenomic databases
HOGENOM	P0A586.
OMA	P0A586. TGDLAFK.
Enzyme and pathway databases
BRENDA	1.1.1.49. 809.
Family and domain databases
InterPro	IPR001282. Glc-6-P_DH. IPR019796. Glc-6-P_DH_AS. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR23429. G6PDH. 1 hit.
Pfam	PF02781. G6PD_C. 1 hit. PF00479. G6PD_N. 1 hit. [Graphical view]
PIRSF	PIRSF000110. G6PD. 1 hit.
PRINTS	PR00079. G6PDHDRGNASE.
ProDom	PD001129. G6PD. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00871. zwf. 1 hit.
PROSITE	PS00069. G6P_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G6PD2_MYCTU

Accession

Primary (citable) accession number: P0A586
Secondary accession number(s): O06573

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	June 16, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)