Reviewed, UniProtKB/Swiss-Prot P0A584 (G6PD_MYCTU)

Names and origin

Protein names

Recommended name:
    Glucose-6-phosphate 1-dehydrogenase
      Short name=G6PD
    EC=1.1.1.49

Gene names

Name:	zwf
Synonyms:	zwf2
Ordered Locus Names:	Rv1447c, MT1494
ORF Names:	MTCY493.07

Organism

Mycobacterium tuberculosis [Complete proteome] [HAMAP]

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	514 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	D-glucose 6-phosphate + NADP+ = D-glucono-1,5-lactone 6-phosphate + NADPH.
Pathway	Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.
Sequence similarities	Belongs to the glucose-6-phosphate dehydrogenase family.
Caution	M.tuberculosis has two genes for zwf. This one looks like a classical zwf.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glucose metabolism
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro glucose-6-phosphate dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 514	514	Glucose-6-phosphate 1-dehydrogenase		PRO_0000068126
Sites
	Active site	268	1	Proton acceptor By similarity
	Binding site	37	1	NADP By similarity
	Binding site	69	1	NADP By similarity
	Binding site	206	1	Substrate By similarity
	Binding site	210	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A584-1 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 22A9CCEDC9AB062F	FASTA	514	57,343
	10 20 30 40 50 60 MKPAHAAASW RNPLRDKRDK RLPRIAGPCG MVIFGVTGDL ARKKVMPAVY DLANRGLLPP 70 80 90 100 110 120 TFSLVGFARR DWSTQDFGQV VYNAVQEHCR TPFRQQNWDR LAEGFRFVPG TFDDDDAFAQ 130 140 150 160 170 180 LAETLEKLDA ERGTGGNHAF YLAIPPKSFP VVCEQLHKSG LARPQGDRWS RVVIEKPFGH 190 200 210 220 230 240 DLASARELNK AVNAVFPEEA VFRIDHYLGK ETVQNILALR FANQLFDPIW NAHYVDHVQI 250 260 270 280 290 300 TMAEDIGLGG RAGYYDGIGA ARDVIQNHLM QLLALTAMEE PVSFHPAALQ AEKIKVLSAT 310 320 330 340 350 360 RLAEPLDQTT SRGQYAAGWQ GGEKVVGLLD EEGFAEDSTT ETFAAITLEV DTRRWAGVPF 370 380 390 400 410 420 YLRTGKRLGR RVTEIALVFR RAPHLPFDAT MTDELGTNAM VIRVQPDEGV TLRFGSKVPG 430 440 450 460 470 480 TAMEVRDVNM DFSYGSAFAE DSPEAYERLI LDVLLGEPSL FPVNAEVELA WEILDPALEH 490 500 510 WAAHGTPDAY EAGTWGPESS LEMLRRTGRE WRRP

References

[1]

"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.

[2]

"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX842576 Genomic DNA. Translation: CAB09259.1. AE000516 Genomic DNA. Translation: AAK45757.1.
PIR	B70917.
RefSeq	NP_215963.1. NP_335943.1.
3D structure databases
HSSP	HSSP built from PDB template 1QKI based on UniProtKB P11413.
ModBase	Search...
Genome annotation databases
GeneID	886614. 924476.
GenomeReviews	Gene locus MT1494 in contig AE000516_GR. Gene locus Rv1447c in contig AL123456_GR.
KEGG	mtc:MT1494. mtu:Rv1447c.
TIGR	MT1494.
Organism-specific databases
TubercuList	Rv1447c.
Phylogenomic databases
HOGENOM	P0A584.
OMA	P0A584. SRRWAGV.
Enzyme and pathway databases
BRENDA	1.1.1.49. 809.
Family and domain databases
InterPro	IPR001282. Glc-6-P_DH. IPR019796. Glc-6-P_DH_AS. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR23429. G6PDH. 1 hit.
Pfam	PF02781. G6PD_C. 1 hit. PF00479. G6PD_N. 1 hit. [Graphical view]
PIRSF	PIRSF000110. G6PD. 1 hit.
PRINTS	PR00079. G6PDHDRGNASE.
ProDom	PD001129. G6PD. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00871. zwf. 1 hit.
PROSITE	PS00069. G6P_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G6PD_MYCTU

Accession

Primary (citable) accession number: P0A584
Secondary accession number(s): O08407

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	June 16, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)