6 kDa early secretory antigenic target - Mycobacterium tuberculosis

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P0A564 (ESXA_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 47. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
6 kDa early secretory antigenic target

Alternative name(s):
ESAT-6

Gene names

Name:	esxA
Synonyms:	esaT6
Ordered Locus Names:	Rv3875, MT3989
ORF Names:	MTV027.10

Organism

Mycobacterium tuberculosis

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	95 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Not known. Elicits high level of IFN-gamma from memory effector cells during the first phase of a protective immune response.
Subunit structure	Forms a tight 1:1 complex with EsxB (CFP-10). Interacts with EspF. Ref.7 Ref.8 Ref.11
Subcellular location	Secreted. Note: Probably secreted via the ESX-1 / type VII secretion system (T7SS). Ref.8
Disruption phenotype	Inactivation leads to absence of EsxA and EsxB from cell lysates. Ref.10
Miscellaneous	Secretion of EspA, EsxA and EsxB is mutually dependent.
Sequence similarities	Belongs to the ESAT-6 (esx) family.

Ontologies

Keywords
Cellular component	Secreted
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	modulation by symbiont of host signal transduction pathway Inferred from mutant phenotype Ref.8. Source: MTBBASE negative regulation by symbiont of host cell-mediated immune response Inferred from mutant phenotype Ref.8. Source: MTBBASE pathogenesis Inferred from mutant phenotype. Source: MTBBASE protein secretion by the type VII secretion system Inferred from mutant phenotype. Source: MTBBASE
Cellular component	cell wall Inferred from direct assay Ref.2. Source: MTBBASE cytoplasm Inferred from direct assay Ref.2. Source: MTBBASE extracellular region Inferred from direct assay Ref.8. Source: MTBBASE plasma membrane Inferred from direct assay. Source: MTBBASE
Molecular function	host cell surface binding Inferred from direct assay. Source: MTBBASE protein homodimerization activity Inferred from physical interaction. Source: MTBBASE
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
esxB	P0A566	3	EBI-1253925,EBI-1253936

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed Ref.2

Chain

2 – 95

6 kDa early secretory antigenic target

PRO_0000167793

Secondary structure

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P0A564 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 79BD529E3D88F519
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FASTA

9,904

        10         20         30         40         50         60 
MTEQQWNFAG IEAAASAIQG NVTSIHSLLD EGKQSLTKLA AAWGGSGSEA YQGVQQKWDA 

        70         80         90 
TATELNNALQ NLARTISEAG QAMASTEGNV TGMFA

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Recall of long-lived immunity to Mycobacterium tuberculosis infection in mice." Andersen P., Andersen A.B., Sorensen A.L., Nagai S. J. Immunol. 154:3359-3372(1995) [PubMed: 7897219] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[2]	"Purification and characterization of a low-molecular-mass T-cell antigen secreted by Mycobacterium tuberculosis." Soerensen A.L., Nagai S., Houen G., Anderson P., Anderson A.B. Infect. Immun. 63:1710-1717(1995) [PubMed: 7729876] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-11, CHARACTERIZATION. Strain: ATCC 35801 / TMC 107 / Erdman.
[3]	"ESAT-6 gene of a clinical isolate of Mycobacterium tuberculosis from India." Singh B., Siddiqui Z., Singh S., Sharma P. Submitted (SEP-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[5]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
[6]	"A Mycobacterium tuberculosis operon encoding ESAT-6 and a novel low-molecular-mass culture filtrate protein (CFP-10)." Berthet F.-X., Rasmussen P.B., Rosenkrands I., Andersen P., Gicquel B. Microbiology 144:3195-3203(1998) [PubMed: 9846755] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-71. Strain: ATCC 25618 / H37Rv.
[7]	"Conclusive evidence that the major T-cell antigens of the Mycobacterium tuberculosis complex ESAT-6 and CFP-10 form a tight, 1:1 complex and characterization of the structural properties of ESAT-6, CFP-10, and the ESAT-6*CFP-10 complex. Implications for pathogenesis and virulence." Renshaw P.S., Panagiotidou P., Whelan A., Gordon S.V., Hewinson R.G., Williamson R.A., Carr M.D. J. Biol. Chem. 277:21598-21603(2002) [PubMed: 11940590] [Abstract] Cited for: SUBUNIT, INTERACTION WITH ESXB.
[8]	"Acute infection and macrophage subversion by Mycobacterium tuberculosis require a specialized secretion system." Stanley S.A., Raghavan S., Hwang W.W., Cox J.S. Proc. Natl. Acad. Sci. U.S.A. 100:13001-13006(2003) [PubMed: 14557536] [Abstract] Cited for: INTERACTION WITH ESXB, SUBCELLULAR LOCATION.
[9]	"Mutually dependent secretion of proteins required for mycobacterial virulence." Fortune S.M., Jaeger A., Sarracino D.A., Chase M.R., Sassetti C.M., Sherman D.R., Bloom B.R., Rubin E.J. Proc. Natl. Acad. Sci. U.S.A. 102:10676-10681(2005) [PubMed: 16030141] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY. Strain: ATCC 25618 / H37Rv.
[10]	"Dissection of ESAT-6 system 1 of Mycobacterium tuberculosis and impact on immunogenicity and virulence." Brodin P., Majlessi L., Marsollier L., de Jonge M.I., Bottai D., Demangel C., Hinds J., Neyrolles O., Butcher P.D., Leclerc C., Cole S.T., Brosch R. Infect. Immun. 74:88-98(2006) [PubMed: 16368961] [Abstract] Cited for: DISRUPTION PHENOTYPE.
[11]	"ESX-1 secreted virulence factors are recognized by multiple cytosolic AAA ATPases in pathogenic mycobacteria." DiGiuseppe Champion P.A., Champion M.M., Manzanillo P., Cox J.S. Mol. Microbiol. 73:950-962(2009) [PubMed: 19682254] [Abstract] Cited for: INTERACTION WITH ESPF.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X79562 Genomic DNA. Translation: CAA56099.1.
AF420491 Genomic DNA. Translation: AAL16896.1.
BX842584 Genomic DNA. Translation: CAE55648.1.
AE000516 Genomic DNA. Translation: AAK48357.1.
AF004671 Genomic DNA. Translation: AAC83446.1.

PIR

A70803.

RefSeq

NP_338543.1. NC_002755.2.
YP_178023.1. NC_000962.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1WA8	NMR	-	B	1-95	[»]
3FAV	X-ray	2.15	B/D	2-95	[»]

ProteinModelPortal

P0A564.

SMR

P0A564. Positions 1-95.

ModBase

Search...

Protein-protein interaction databases

IntAct

P0A564. 2 interactions.

Protein family/group databases

TCDB

1.C.95.1.1. pore-forming ESAT-6 protein (ESAT-6) family.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBMYCT00000000085; EBMYCP00000000085; EBMYCG00000000085.
EBMYCT00000072395; EBMYCP00000070454; EBMYCG00000072390.

GeneID

886209.
922546.

GenomeReviews

Gene locus MT3989 in contig AE000516_GR.
Gene locus Rv3875 in contig AL123456_GR.

KEGG

mtc:MT3989.
mtu:Rv3875.

PATRIC

18130531. VBIMycTub22151_4353.

TIGR

MT3989.

Organism-specific databases

TubercuList

Rv3875.

Phylogenomic databases

GeneTree

EBGT00050000016159.

HOGENOM

HBG569144.

OMA

NTEGINH.

ProtClustDB

CLSK800028.

Family and domain databases

InterPro

IPR010310. T7SS_ESAT-6-like.
[Graphical view]

K14956.

Pfam

PF06013. WXG100. 1 hit.
[Graphical view]

TIGRFAMs

TIGR03930. WXG100_ESAT6. 1 hit.

ProtoNet

Search...

Entry information

Entry name

ESXA_MYCTU

Accession

Primary (citable) accession number: P0A564
Secondary accession number(s): O84901, Q57165

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 47 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents