Probable arabinosyltransferase A - Mycobacterium tuberculosis

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P0A560 (EMBA_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 57. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Probable arabinosyltransferase A
EC=2.4.2.-

Gene names

Name:	embA
Ordered Locus Names:	Rv3794, MT3901
ORF Names:	MTCY13D12.28

Organism

Mycobacterium tuberculosis [Reference proteome] [HAMAP]

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	1094 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Arabinosyl transferase responsible for the polymerization of arabinose into the arabinan of arabinogalactan.
Subcellular location	Cell membrane; Multi-pass membrane protein Probable.
Induction	Positively regulated by the transcriptional regulatory protein EmbR. Ref.4
Miscellaneous	This is one of the target of the anti-tuberculosis drug ethambutol [(S,S')-2,2'-(ethylenediimino)di-1-butanol; EMB]. EMB is a first-line drug used to treat tuberculosis. EMB inhibits the transfer of arabinogalactan into the cell wall. Was identified as a high-confidence drug target.
Sequence similarities	Belongs to the emb family.

Ontologies

Keywords
Biological process	Antibiotic resistance Cell wall biogenesis/degradation
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Actinobacterium-type cell wall biogenesis Inferred from electronic annotation. Source: InterPro growth Inferred from mutant phenotype PubMed 18174142. Source: MTBBASE response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytosol Inferred from direct assay PubMed 15525680. Source: MTBBASE extracellular region Inferred from genetic interaction PubMed 10781540. Source: MTBBASE integral to plasma membrane Inferred from direct assay PubMed 15952732. Source: MTBBASE
Molecular_function	arabinosyltransferase activity Inferred from direct assay PubMed 18174142. Source: MTBBASE
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1094

1094

Probable arabinosyltransferase A

PRO_0000220564

Regions

Transmembrane

12 – 34

Helical; Potential

Transmembrane

205 – 224

Helical; Potential

Transmembrane

247 – 269

Helical; Potential

Transmembrane

322 – 344

Helical; Potential

Transmembrane

356 – 375

Helical; Potential

Transmembrane

408 – 430

Helical; Potential

Transmembrane

451 – 470

Helical; Potential

Transmembrane

519 – 536

Helical; Potential

Transmembrane

543 – 565

Helical; Potential

Transmembrane

575 – 597

Helical; Potential

Transmembrane

604 – 626

Helical; Potential

Transmembrane

641 – 663

Helical; Potential

Transmembrane

684 – 706

Helical; Potential

Natural variations

Natural variant

201

A → T Resistance to EMB. Ref.6

Natural variant

321

G → S Resistance to EMB. Ref.6

Natural variant

350

G → D Resistance to EMB. Ref.6

Natural variant

462

A → V Resistance to EMB. Ref.6

Natural variant

833

D → A Resistance to EMB. Ref.6

Natural variant

913

P → S Resistance to EMB. Ref.6

Experimental info

Sequence conflict

116

Missing in AAC45280. Ref.1

Sequence conflict

899 – 900

TG → HR in AAC45280. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

P0A560 [UniParc].

Last modified March 15, 2005. Version 1.
Checksum: 11EEA34999633EEC
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FASTA

1,094

115,724

        10         20         30         40         50         60 
MPHDGNERSH RIARLAAVVS GIAGLLLCGI VPLLPVNQTT ATIFWPQGST ADGNITQITA 

        70         80         90        100        110        120 
PLVSGAPRAL DISIPCSAIA TLPANGGLVL STLPAGGVDT GKAGLFVRAN QDTVVVAFRD 

       130        140        150        160        170        180 
SVAAVAARST IAAGGCSALH IWADTGGAGA DFMGIPGGAG TLPPEKKPQV GGIFTDLKVG 

       190        200        210        220        230        240 
AQPGLSARVD IDTRFITTPG ALKKAVMLLG VLAVLVAMVG LAALDRLSRG RTLRDWLTRY 

       250        260        270        280        290        300 
RPRVRVGFAS RLADAAVIAT LLLWHVIGAT SSDDGYLLTV ARVAPKAGYV ANYYRYFGTT 

       310        320        330        340        350        360 
EAPFDWYTSV LAQLAAVSTA GVWMRLPATL AGIACWLIVS RFVLRRLGPG PGGLASNRVA 

       370        380        390        400        410        420 
VFTAGAVFLS AWLPFNNGLR PEPLIALGVL VTWVLVERSI ALGRLAPAAV AIIVATLTAT 

       430        440        450        460        470        480 
LAPQGLIALA PLLTGARAIA QRIRRRRATD GLLAPLAVLA AALSLITVVV FRDQTLATVA 

       490        500        510        520        530        540 
ESARIKYKVG PTIAWYQDFL RYYFLTVESN VEGSMSRRFA VLVLLFCLFG VLFVLLRRGR 

       550        560        570        580        590        600 
VAGLASGPAW RLIGTTAVGL LLLTFTPTKW AVQFGAFAGL AGVLGAVTAF TFARIGLHSR 

       610        620        630        640        650        660 
RNLTLYVTAL LFVLAWATSG INGWFYVGNY GVPWYDIQPV IASHPVTSMF LTLSILTGLL 

       670        680        690        700        710        720 
AAWYHFRMDY AGHTEVKDNR RNRILASTPL LVVAVIMVAG EVGSMAKAAV FRYPLYTTAK 

       730        740        750        760        770        780 
ANLTALSTGL SSCAMADDVL AEPDPNAGML QPVPGQAFGP DGPLGGISPV GFKPEGVGED 

       790        800        810        820        830        840 
LKSDPVVSKP GLVNSDASPN KPNAAITDSA GTAGGKGPVG INGSHAALPF GLDPARTPVM 

       850        860        870        880        890        900 
GSYGENNLAA TATSAWYQLP PRSPDRPLVV VSAAGAIWSY KEDGDFIYGQ SLKLQWGVTG 

       910        920        930        940        950        960 
PDGRIQPLGQ VFPIDIGPQP AWRNLRFPLA WAPPEADVAR IVAYDPNLSP EQWFAFTPPR 

       970        980        990       1000       1010       1020 
VPVLESLQRL IGSATPVLMD IATAANFPCQ RPFSEHLGIA ELPQYRILPD HKQTAASSNL 

      1030       1040       1050       1060       1070       1080 
WQSSSTGGPF LFTQALLRTS TIATYLRGDW YRDWGSVEQY HRLVPADQAP DAVVEEGVIT 

      1090 
VPGWGRPGPI RALP

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The emb operon, a gene cluster of Mycobacterium tuberculosis involved in resistance to ethambutol." Telenti A., Philipp W.J., Sreevatsan S., Bernasconi C., Stockbauer K.E., Wieles B., Musser J.M., Jacobs W.R. Jr. Nat. Med. 3:567-570(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[2]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. Barrell B.G. Nature 393:537-544(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[3]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
[4]	"Transcriptional control of the mycobacterial embCAB operon by PknH through a regulatory protein, EmbR, in vivo." Sharma K., Gupta M., Pathak M., Gupta N., Koul A., Sarangi S., Baweja R., Singh Y. J. Bacteriol. 188:2936-2944(2006) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
[5]	"targetTB: a target identification pipeline for Mycobacterium tuberculosis through an interactome, reactome and genome-scale structural analysis." Raman K., Yeturu K., Chandra N. BMC Syst. Biol. 2:109-109(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION AS A DRUG TARGET [LARGE SCALE ANALYSIS].
[6]	"Molecular genetic analysis of nucleotide polymorphisms associated with ethambutol resistance in human isolates of Mycobacterium tuberculosis." Ramaswamy S.V., Amin A.G., Goeksel S., Stager C.E., Dou S.-J., El Sahly H., Moghazeh S.L., Kreiswirth B.N., Musser J.M. Antimicrob. Agents Chemother. 44:326-336(2000) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANTS EMB RESISTANT THR-201; SER-321; ASP-350; VAL-462; ALA-833 AND SER-913.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U68480 Genomic DNA. Translation: AAC45280.1. BX842584 Genomic DNA. Translation: CAB02473.1. AE000516 Genomic DNA. Translation: AAK48267.1. AL123456 Genomic DNA. Translation: CCP46623.1.
PIR	F70697.
RefSeq	NP_218311.1. NC_000962.3. NP_338453.1. NC_002755.2. YP_006517291.1. NC_018143.1.
3D structure databases
ProteinModelPortal	P0A560.
SMR	P0A560. Positions 41-197.
ModBase	Search...
Protein-protein interaction databases
STRING	83332.Rv3794.
Proteomic databases
PRIDE	P0A560.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAK48267; AAK48267; MT3901.
GeneID	13317418. 886123. 922603.
KEGG	mtc:MT3901. mtu:Rv3794. mtv:RVBD_3794.
PATRIC	18130339. VBIMycTub22151_4257.
Organism-specific databases
TubercuList	Rv3794.
Phylogenomic databases
eggNOG	NOG269637.
HOGENOM	HOG000054334.
KO	K11385.
OMA	ISTASVW.
ProtClustDB	CLSK792727.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-15269.
Family and domain databases
InterPro	IPR007680. Mycobac_arabinan_synth. [Graphical view]
Pfam	PF04602. Arabinose_trans. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P0A560.
ChEMBL	CHEMBL1877.
DrugBank	DB00330. Ethambutol.

Entry information

Entry name

EMBA_MYCTU

Accession

Primary (citable) accession number: P0A560
Secondary accession number(s): L0TDK4, P72029, P72060

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	May 1, 2013
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh

Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Natural variations

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Other

Entry information

Relevant documents