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Protein

Dihydrofolate reductase

Gene

folA

Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis (By similarity).By similarity

Catalytic activityi

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.PROSITE-ProRule annotation

Pathwayi: tetrahydrofolate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate.
Proteins known to be involved in this subpathway in this organism are:
  1. Dihydrofolate reductase (folA)
This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei29SubstrateBy similarity1
Binding sitei34SubstrateBy similarity1
Binding sitei62SubstrateBy similarity1
Binding sitei115SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi8 – 9NADPBy similarity2
Nucleotide bindingi16 – 21NADPBy similarity6
Nucleotide bindingi45 – 48NADPBy similarity4
Nucleotide bindingi67 – 70NADPBy similarity4
Nucleotide bindingi96 – 101NADPBy similarity6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processOne-carbon metabolism
LigandNADP

Enzyme and pathway databases

UniPathwayiUPA00077; UER00158

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydrofolate reductase (EC:1.5.1.3)
Gene namesi
Name:folA
Synonyms:dfrA
Ordered Locus Names:BQ2027_MB2785C
OrganismiMycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Taxonomic identifieri233413 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001419 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001863981 – 161Dihydrofolate reductaseAdd BLAST161

Structurei

3D structure databases

ProteinModelPortaliP0A547
SMRiP0A547
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 160DHFRPROSITE-ProRule annotationAdd BLAST160

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni7 – 9Substrate bindingBy similarity3

Sequence similaritiesi

Belongs to the dihydrofolate reductase family.Curated

Phylogenomic databases

HOGENOMiHOG000040234

Family and domain databases

CDDicd00209 DHFR, 1 hit
Gene3Di3.40.430.10, 1 hit
InterProiView protein in InterPro
IPR012259 DHFR
IPR024072 DHFR-like_dom_sf
IPR017925 DHFR_CS
IPR001796 DHFR_dom
PANTHERiPTHR22778:SF16 PTHR22778:SF16, 1 hit
PfamiView protein in Pfam
PF00186 DHFR_1, 1 hit
PIRSFiPIRSF000194 DHFR, 1 hit
SUPFAMiSSF53597 SSF53597, 1 hit
PROSITEiView protein in PROSITE
PS00075 DHFR_1, 1 hit
PS51330 DHFR_2, 1 hit

Sequencei

Sequence statusi: Complete.

P0A547-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTMVGLIWAQ ATSGVIGRGG DIPWRLPEDQ AHFREITMGH TIVMGRRTWD
60 70 80 90 100
SLPAKVRPLP GRRNVVLSRQ ADFMASGAEV VGSLEEALTS PETWVIGGGQ
110 120 130 140 150
VYALALPYAT RCEVTEVDIG LPREAGDALA PVLDETWRGE TGEWRFSRSG
160
LRYRLYSYHR S
Length:161
Mass (Da):17,872
Last modified:April 1, 2015 - v2
Checksum:iB8980922D2F18C44
GO

Sequence cautioni

The sequence SIU01403 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
LT708304 Genomic DNA Translation: SIU01403.1 Different initiation.
RefSeqiNP_856431.1, NC_002945.3

Genome annotation databases

EnsemblBacteriaiCDO44049; CDO44049; Mb2785c
PATRICifig|233413.5.peg.3052

Similar proteinsi

Entry informationi

Entry nameiDYR_MYCBO
AccessioniPrimary (citable) accession number: P0A547
Secondary accession number(s): A0A1R3Y228, O33305, X2BLG2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: April 1, 2015
Last modified: April 25, 2018
This is version 89 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

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