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P0A547 (DYR_MYCBO) Reviewed, UniProtKB/Swiss-Prot

Last modified December 11, 2013. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dihydrofolate reductase

EC=1.5.1.3
Gene names
Name:folA
Synonyms:dfrA
Ordered Locus Names:Mb2785c
OrganismMycobacterium bovis (strain ATCC BAA-935 / AF2122/97) [Complete proteome] [HAMAP]
Taxonomic identifier233413 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex

Protein attributes

Sequence length159 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis By similarity.

Catalytic activity

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.

Pathway

Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1.

Sequence similarities

Belongs to the dihydrofolate reductase family.

Contains 1 DHFR (dihydrofolate reductase) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 159159Dihydrofolate reductase
PRO_0000186398

Regions

Domain1 – 158158DHFR
Nucleotide binding6 – 72NADP By similarity
Nucleotide binding14 – 196NADP By similarity
Nucleotide binding43 – 464NADP By similarity
Nucleotide binding65 – 684NADP By similarity
Nucleotide binding94 – 996NADP By similarity
Region5 – 73Substrate binding By similarity

Sites

Binding site271Substrate By similarity
Binding site321Substrate By similarity
Binding site601Substrate By similarity
Binding site1131Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
P0A547 [UniParc].

Last modified March 15, 2005. Version 1.
Checksum: F17C038F1B773797

FASTA15917,640
        10         20         30         40         50         60 
MVGLIWAQAT SGVIGRGGDI PWRLPEDQAH FREITMGHTI VMGRRTWDSL PAKVRPLPGR 

        70         80         90        100        110        120 
RNVVLSRQAD FMASGAEVVG SLEEALTSPE TWVIGGGQVY ALALPYATRC EVTEVDIGLP 

       130        140        150 
REAGDALAPV LDETWRGETG EWRFSRSGLR YRLYSYHRS 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BX248343 Genomic DNA. Translation: CAD94970.1.
RefSeqNP_856431.1. NC_002945.3.

3D structure databases

ProteinModelPortalP0A547.
SMRP0A547. Positions 1-159.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING233413.Mb2785c.

Chemistry

BindingDBP0A547.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAD94970; CAD94970; Mb2785c.
GeneID1091594.
KEGGmbo:Mb2785c.
PATRIC18007839. VBIMycBov88188_3052.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0262.
HOGENOMHOG000040234.
KOK00287.
OMAGLRYRFH.
OrthoDBEOG6KT2V2.
ProtClustDBCLSK792062.

Enzyme and pathway databases

UniPathwayUPA00077; UER00158.

Family and domain databases

Gene3D3.40.430.10. 1 hit.
InterProIPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
[Graphical view]
PfamPF00186. DHFR_1. 1 hit.
[Graphical view]
PIRSFPIRSF000194. DHFR. 1 hit.
PRINTSPR00070. DHFR.
SUPFAMSSF53597. SSF53597. 1 hit.
PROSITEPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDYR_MYCBO
AccessionPrimary (citable) accession number: P0A547
Secondary accession number(s): O33305
Entry history
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: December 11, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways