Reviewed, UniProtKB/Swiss-Prot P0A543 (DHAS_MYCBO)

Names and origin

Protein names

Recommended name:
    Aspartate-semialdehyde dehydrogenase
      Short name=ASA dehydrogenase
      Short name=ASADH
    EC=1.2.1.11

Gene names

Name:	asd
Ordered Locus Names:	Mb3735c

Organism

Mycobacterium bovis [Complete proteome] [HAMAP]

Taxonomic identifier

1765 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	345 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-aspartate 4-semialdehyde + phosphate + NADP+ = L-4-aspartyl phosphate + NADPH.
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; tetrahydrodipicolinate from L-aspartate: step 2/4. Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 2/3. Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 2/5.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the aspartate-semialdehyde dehydrogenase family.
Sequence caution	The sequence Z18290 differs from that shown. Reason: Frameshift at position 313.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW methionine biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW threonine biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro NADP or NADPH binding Inferred from electronic annotation. Source: InterPro aspartate-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: EC protein dimerization activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 345	345	Aspartate-semialdehyde dehydrogenase		PRO_0000141381
Sites
	Active site	130	1	Acyl-thioester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A543-1 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: C886DDB63D03E25D	FASTA	345	36,230
	10 20 30 40 50 60 MGLSIGIVGA TGQVGQVMRT LLDERDFPAS AVRFFASARS QGRKLAFRGQ EIEVEDAETA 70 80 90 100 110 120 DPSGLDIALF SAGSAMSKVQ APRFAAAGVT VIDNSSAWRK DPDVPLVVSE VNFERDAHRR 130 140 150 160 170 180 PKGIIANPNC TTMAAMPVLK VLHDEARLVR LVVSSYQAVS GSGLAGVAEL AEQARAVIGG 190 200 210 220 230 240 AEQLVYDGGA LEFPPPNTYV APIAFNVVPL AGSLVDDGSG ETDEDQKLRF ESRKILGIPD 250 260 270 280 290 300 LLVSGTCVRV PVFTGHSLSI NAEFAQPLSP ERARELLDGA TGVQLVDVPT PLAAAGVDES 310 320 330 340 LVGRIRRDPG VPDGRGLALF VSGDNLRKGA ALNTIQIAEL LTADL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of the aspartokinase and aspartate semialdehyde dehydrogenase operon from mycobacteria." Cirillo J.D., Weisbrod T.R., Pascopella L., Bloom B.R., Jacobs W.R. Jr. Mol. Microbiol. 11:629-639(1994) [PubMed: 7910936] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: BCG / Pasteur.
[2]	"The complete genome sequence of Mycobacterium bovis." Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M., Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B., Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J. , Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G. Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003) [PubMed: 12788972] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-935 / AF2122/97.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z18290 Genomic DNA. No translation available. BX248347 Genomic DNA. Translation: CAD95921.1.
RefSeq	NP_857373.1.
3D structure databases
HSSP	HSSP built from PDB template 1BRM based on UniProtKB P00353.
ModBase	Search...
Genome annotation databases
GeneID	1093103.
GenomeReviews	Gene locus Mb3735c in contig BX248333_GR.
KEGG	mbo:Mb3735c.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P0A543.
OMA	P0A543. LELWLCG.
Enzyme and pathway databases
BRENDA	1.2.1.11. 3091.
Family and domain databases
InterPro	IPR000319. Asp-semialdehyde_DH_CS. IPR012080. Asp_semialdehyde_DH. IPR005986. Asp_semialdehyde_DH_bac. IPR000534. Semialdehyde_DH_NAD-bd. IPR012280. Semialdhyde_DH_C. [Graphical view]
Pfam	PF01118. Semialdhyde_dh. 1 hit. PF02774. Semialdhyde_dhC. 1 hit. [Graphical view]
PIRSF	PIRSF000148. ASA_dh. 1 hit.
TIGRFAMs	TIGR01296. asd_B. 1 hit.
PROSITE	PS01103. ASD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHAS_MYCBO

Accession

Primary (citable) accession number: P0A543
Secondary accession number(s): P47730, P97049

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	June 16, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)