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Protein

Purine nucleoside phosphorylase

Gene

punA

Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

The purine nucleoside phosphorylases catalyze the phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate. Cleaves guanosine, inosine, 2'-deoxyguanosine and 2'-deoxyinosine.By similarity

Catalytic activityi

Purine deoxynucleoside + phosphate = purine + 2'-deoxy-alpha-D-ribose 1-phosphate.By similarity

Pathwayi: purine nucleoside salvage

This protein is involved in the pathway purine nucleoside salvage, which is part of Purine metabolism.
View all proteins of this organism that are known to be involved in the pathway purine nucleoside salvage and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei36PhosphateBy similarity1
Binding sitei68PhosphateBy similarity1
Binding sitei120Phosphate; via amide nitrogenBy similarity1
Binding sitei189Purine nucleosideBy similarity1
Binding sitei208PhosphateBy similarity1
Binding sitei231Purine nucleosideBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciMBOV233413:G1GT4-3503-MONOMER
UniPathwayiUPA00606

Names & Taxonomyi

Protein namesi
Recommended name:
Purine nucleoside phosphorylase (EC:2.4.2.1)
Short name:
PNP
Short name:
Pu-NPase
Alternative name(s):
Inosine phosphorylase
Inosine-guanosine phosphorylase
Gene namesi
Name:punA
Synonyms:deoD
Ordered Locus Names:BQ2027_MB3335
OrganismiMycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Taxonomic identifieri233413 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001419 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001845421 – 268Purine nucleoside phosphorylaseAdd BLAST268

Interactioni

Subunit structurei

Homotrimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP0A539
SMRiP0A539
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni88 – 90Phosphate bindingBy similarity3

Sequence similaritiesi

Belongs to the PNP/MTAP phosphorylase family.Curated

Phylogenomic databases

HOGENOMiHOG000045183
OMAiVLISDHI

Family and domain databases

InterProiView protein in InterPro
IPR000845 Nucleoside_phosphorylase_d
IPR035994 Nucleoside_phosphorylase_sf
IPR011269 PUNP
IPR011268 Purine_phosphorylase
IPR018099 Purine_phosphorylase-2_CS
PANTHERiPTHR11904 PTHR11904, 1 hit
PfamiView protein in Pfam
PF01048 PNP_UDP_1, 1 hit
PIRSFiPIRSF000477 PurNPase, 1 hit
SUPFAMiSSF53167 SSF53167, 1 hit
TIGRFAMsiTIGR01697 PNPH-PUNA-XAPA, 1 hit
TIGR01698 PUNP, 1 hit
PROSITEiView protein in PROSITE
PS01240 PNP_MTAP_2, 1 hit

Sequencei

Sequence statusi: Complete.

P0A539-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADPRPDPDE LARRAAQVIA DRTGIGEHDV AVVLGSGWLP AVAALGSPTT
60 70 80 90 100
VLPQAELPGF VPPTAAGHAG ELLSVPIGAH RVLVLAGRIH AYEGHDLRYV
110 120 130 140 150
VHPVRAARAA GAQIMVLTNA AGGLRADLQV GQPVLISDHL NLTARSPLVG
160 170 180 190 200
GEFVDLTDAY SPRLRELARQ SDPQLAEGVY AGLPGPHYET PAEIRMLQTL
210 220 230 240 250
GADLVGMSTV HETIAARAAG AEVLGVSLVT NLAAGITGEP LSHAEVLAAG
260
AASATRMGAL LADVIARF
Length:268
Mass (Da):27,572
Last modified:March 15, 2005 - v1
Checksum:i12AA429779CE89F6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
LT708304 Genomic DNA Translation: SIU01964.1
RefSeqiNP_856980.1, NC_002945.3
WP_003417233.1, NC_002945.4

Genome annotation databases

EnsemblBacteriaiCDO44607; CDO44607; Mb3335
PATRICifig|233413.5.peg.3665

Similar proteinsi

Entry informationi

Entry nameiPUNA_MYCBO
AccessioniPrimary (citable) accession number: P0A539
Secondary accession number(s): A0A1R3Y3R6, O53359, X2BMT9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: April 25, 2018
This is version 83 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

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