P0A530 (COAD_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphopantetheine adenylyltransferase
EC=2.7.7.3

Alternative name(s):
Dephospho-CoA pyrophosphorylase
Pantetheine-phosphate adenylyltransferase
Short name=PPAT

Gene names

Name:	coaD
Synonyms:	kdtB
Ordered Locus Names:	Rv2965c, MT3043
ORF Names:	MTCY349.22, u0002e

Organism

Mycobacterium tuberculosis

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	161 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Reversibly transfers an adenylyl group from ATP to 4'-phosphopantetheine, yielding dephospho-CoA (dPCoA) and pyrophosphate By similarity. HAMAP MF_00151
Catalytic activity	ATP + pantetheine 4'-phosphate = diphosphate + 3'-dephospho-CoA. HAMAP MF_00151
Pathway	Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 4/5. HAMAP MF_00151
Subunit structure	Homohexamer By similarity. HAMAP MF_00151
Subcellular location	Cytoplasm By similarity HAMAP MF_00151.
Sequence similarities	Belongs to the bacterial CoaD family.

Ontologies

Keywords
Biological process	Coenzyme A biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	coenzyme A biosynthetic process Inferred from direct assay. Source: MTBBASE protein hexamerization Inferred from direct assay. Source: MTBBASE
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW pantetheine-phosphate adenylyltransferase activity Inferred from direct assay. Source: MTBBASE
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 161

161

Phosphopantetheine adenylyltransferase HAMAP MF_00151

PRO_0000156242

Secondary structure

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161

Helix Strand Turn

Details...

Beta strand

3 – 8

6

Helix

15 – 27

13

Beta strand

28 – 36

9

Beta strand

39 – 41

3

Helix

47 – 57

11

Beta strand

64 – 69

6

Helix

73 – 79

7

Beta strand

84 – 89

6

Helix

95 – 109

15

Beta strand

112 – 117

6

Helix

120 – 122

3

Helix

127 – 135

9

Helix

141 – 143

3

Helix

146 – 155

10

Sequences

	Sequence		Length	Mass (Da)
	P0A530 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: DE9F12E18D0C1721	FASTA	161	17,628
	10 20 30 40 50 60 MTGAVCPGSF DPVTLGHVDI FERAAAQFDE VVVAILVNPA KTGMFDLDER IAMVKESTTH 70 80 90 100 110 120 LPNLRVQVGH GLVVDFVRSC GMTAIVKGLR TGTDFEYELQ MAQMNKHIAG VDTFFVATAP 130 140 150 160 RYSFVSSSLA KEVAMLGGDV SELLPEPVNR RLRDRLNTER T

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Smith D.R., Robison K. Submitted (SEP-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[3]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U00024 Genomic DNA. Translation: AAA50946.1.
BX842581 Genomic DNA. Translation: CAB05412.1.
AE000516 Genomic DNA. Translation: AAK47369.1.

PIR

B70671.

RefSeq

NP_217481.1. NC_000962.2.
NP_337555.1. NC_002755.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1TFU	X-ray	1.99	A	1-157	[»]
3LCJ	X-ray	2.10	A	1-161	[»]
3NBA	X-ray	2.68	A/B/C/D	1-157	[»]
3NBK	X-ray	1.58	A/B/C/D	1-157	[»]
3PNB	X-ray	2.11	A/B/C/D	1-157	[»]

ProteinModelPortal

P0A530.

SMR

P0A530. Positions 1-157.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBMYCT00000000913; EBMYCP00000000913; EBMYCG00000000913.
EBMYCT00000070721; EBMYCP00000068780; EBMYCG00000070716.

GeneID

888423.
925233.

GenomeReviews

Gene locus MT3043 in contig AE000516_GR.
Gene locus Rv2965c in contig AL123456_GR.

KEGG

mtc:MT3043.
mtu:Rv2965c.

PATRIC

18128450. VBIMycTub22151_3325.

TIGR

MT3043.

Organism-specific databases

TubercuList

Rv2965c.

Phylogenomic databases

GeneTree

EBGT00050000017247.

HOGENOM

HBG288308.

OMA

AMSDFEY.

PhylomeDB

P0A530.

ProtClustDB

PRK00168.

Family and domain databases

HAMAP

MF_00151. PPAT_bact.
[Tree]

InterPro

IPR004821. Cyt_trans-rel.
IPR004820. Cytidylyltransf.
IPR001980. LPS_biosynth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]

Gene3D

G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.

KO

K00954.

Pfam

PF01467. CTP_transf_2. 1 hit.
[Graphical view]

PRINTS

PR01020. LPSBIOSNTHSS.

TIGRFAMs

TIGR01510. CoaD_prev_kdtB. 1 hit.
TIGR00125. Cyt_tran_rel. 1 hit.

ProtoNet

Search...

Entry information

Entry name

COAD_MYCTU

Accession

Primary (citable) accession number: P0A530
Secondary accession number(s): O08023, Q50452

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	January 25, 2012
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program