Reviewed, UniProtKB/Swiss-Prot P0A509 (BCCA_MYCBO)

Names and origin

Protein names

Recommended name:
    Acetyl-/propionyl-coenzyme A carboxylase alpha chain
Including the following 2 domains:
    1- Recommended name:
            Biotin carboxylase
              EC=6.3.4.14
    2- Recommended name:
            Biotin carboxyl carrier protein
                Short name=BCCP

Gene names

Name:	accA1
Synonyms:	bccA
Ordered Locus Names:	Mb2529c

Organism

Mycobacterium bovis [Complete proteome] [HAMAP]

Taxonomic identifier

1765 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	654 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This protein carries two functions: biotin carboxyl carrier protein and biotin carboxyltransferase By similarity.
Catalytic activity	ATP + biotin-carboxyl-carrier protein + CO2 = ADP + phosphate + carboxybiotin-carboxyl-carrier protein.
Cofactor	Biotin.
Pathway	Lipid metabolism; fatty acid biosynthesis.
Subunit structure	Multimer comprised of 2 different subunits, the larger one (63/64 kDa) has biotin carboxylase and biotin carrier functions, while the smaller subunit possesses carboxyltransferase and substrate binding activity By similarity.
Sequence similarities	Contains 1 ATP-grasp domain. Contains 1 biotin carboxylation domain. Contains 1 biotinyl-binding domain.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis
Ligand	ATP-binding Biotin Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW biotin binding Inferred from electronic annotation. Source: InterPro biotin carboxylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 654	654	Acetyl-/propionyl-coenzyme A carboxylase alpha chain		PRO_0000146796
Regions
	Domain	1 – 448	448	Biotin carboxylation
	Domain	120 – 319	200	ATP-grasp
	Domain	586 – 652	67	Biotinyl-binding
Sites
	Active site	294	1	By similarity
	Binding site	116	1	ATP By similarity
	Binding site	200	1	ATP By similarity
	Binding site	235	1	ATP By similarity
Amino acid modifications
	Modified residue	620	1	N6-biotinyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A509-1 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: FAA0A1A46432CABF	FASTA	654	70,592
	10 20 30 40 50 60 MFDTVLVANR GEIAVRVIRT LRRLGIRSVA VYSDPDVDAR HVLEADAAVR LGPAPARESY 70 80 90 100 110 120 LDIGKVLDAA ARTGAQAIHP GYGFLAENAD FAAACERARV VFLGPPARAI EVMGDKIAAK 130 140 150 160 170 180 NAVAAFDVPV VPGVARAGLT DDALVTAAAE VGYPVLIKPS AGGGGKGMRL VQDPARLPEA 190 200 210 220 230 240 LVSARREAMS SFGDDTLFLE RFVLRPRHIE VQVLADAHGN VVHLGERECS LQRRHQKVIE 250 260 270 280 290 300 EAPSPLLDPQ TRERIGVAAC NTARCVDYVG AGTVEFIVSA QRPDEFFFME MNTRLQVEHP 310 320 330 340 350 360 VTEAITGLDL VEWQLRVGAG EKLGFAQNDI ELRGHAIEAR VYAEDPAREF LPTGGRVLAV 370 380 390 400 410 420 FEPAGPGVRV DSSLLGGTVV GSDYDPLLTK VIAHGADREE ALDRLDQALA RTAVLGVQTN 430 440 450 460 470 480 VEFLRFLLAD ERVRVGDLDT AVLDERSADF TARPAPDDVL AAGGLYRQWA LARRAQGDLW 490 500 510 520 530 540 AAPSGWRGGG HMAPVRTAMR TPLRSETVSV WGPPESAQVQ VGDGEIDCAS VQVTREQMSV 550 560 570 580 590 600 TISGLRRDYR WAEADRHLWI ADERGTWHLR EAEEHKIHRA VGARPAEVVS PMPGSVIAVQ 610 620 630 640 650 VESGSQISAG DVVVVVEAMK MEHSLEAPVS GRVQVLVSVG DQVKVEQVLA RIKD

References

[1]

"The complete genome sequence of Mycobacterium bovis." Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M., Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B., Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J. , Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G. Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003) [PubMed: 12788972] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-935 / AF2122/97.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX248342 Genomic DNA. Translation: CAD97390.1.
RefSeq	NP_856174.1.
3D structure databases
HSSP	HSSP built from PDB template 1BNC based on UniProtKB P24182.
ModBase	Search...
Genome annotation databases
GeneID	1090579.
GenomeReviews	Gene locus Mb2529c in contig BX248333_GR.
KEGG	mbo:Mb2529c.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P0A509.
OMA	TWHIREA.
Enzyme and pathway databases
BRENDA	6.3.4.14. 3091.
Family and domain databases
InterPro	IPR011761. ATP-grasp. IPR013816. ATP_grasp_subdomain_2. IPR011764. BC. IPR001882. Biotin_BS. IPR005482. Biotin_COase_C. IPR000089. Biotin_lipoyl. IPR005479. CarbamoylP_synth_lsu_ATP-bd. IPR005481. CarbamoylP_synth_lsu_N. IPR013817. Pre-ATP_grasp. [Graphical view]
Gene3D	G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
Pfam	PF02785. Biotin_carb_C. 1 hit. PF00364. Biotin_lipoyl. 1 hit. PF00289. CPSase_L_chain. 1 hit. PF02786. CPSase_L_D2. 1 hit. [Graphical view]
PROSITE	PS50975. ATP_GRASP. 1 hit. PS50979. BC. 1 hit. PS00188. BIOTIN. 1 hit. PS50968. BIOTINYL_LIPOYL. 1 hit. PS00866. CPSASE_1. 1 hit. PS00867. CPSASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BCCA_MYCBO

Accession

Primary (citable) accession number: P0A509
Secondary accession number(s): P46401

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	November 3, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)