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P0A507

- BIOB_MYCBO

UniProt

P0A507 - BIOB_MYCBO

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Protein
Biotin synthase
Gene
bioB, Mb1615
Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi85 – 851Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi89 – 891Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi92 – 921Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi128 – 1281Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi161 – 1611Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi220 – 2201Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi290 – 2901Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:Mb1615
OrganismiMycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Taxonomic identifieri233413 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
ProteomesiUP000001419: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 349349Biotin synthaseUniRule annotation
PRO_0000185558Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi233413.Mb1615.

Structurei

3D structure databases

ProteinModelPortaliP0A507.
SMRiP0A507. Positions 45-340.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239958.
KOiK01012.
OMAiETHHNID.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

P0A507-1 [UniParc]FASTAAdd to Basket

« Hide

MTQAATRPTN DAGQDGGNNS DILVVARQQV LQRGEGLNQD QVLAVLQLPD    50
DRLEELLALA HEVRMRWCGP EVEVEGIISL KTGGCPEDCH FCSQSGLFAS 100
PVRSAWLDIP SLVEAAKQTA KSGATEFCIV AAVRGPDERL MAQVAAGIEA 150
IRNEVEINIA CSLGMLTAEQ VDQLAARGVH RYNHNLETAR SFFANVVTTH 200
TWEERWQTLS MVRDAGMEVC CGGILGMGET LQQRAEFAAE LAELGPDEVP 250
LNFLNPRPGT PFADLEVMPV GDALKAVAAF RLALPRTMLR FAGGREITLG 300
DLGAKRGILG GINAVIVGNY LTTLGRPAEA DLELLDELQM PLKALNASL 349
Length:349
Mass (Da):37,550
Last modified:March 15, 2005 - v1
Checksum:iF3B0A5821B3F14A2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF041819 Genomic DNA. Translation: AAB96962.1.
BX248333 Genomic DNA. Translation: CDO42864.1.
RefSeqiNP_855268.1. NC_002945.3.

Genome annotation databases

EnsemblBacteriaiCAD96283; CAD96283; Mb1615.
GeneIDi1092504.
KEGGimbo:Mb1615.
PATRICi18005233. VBIMycBov88188_1764.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF041819 Genomic DNA. Translation: AAB96962.1 .
BX248333 Genomic DNA. Translation: CDO42864.1 .
RefSeqi NP_855268.1. NC_002945.3.

3D structure databases

ProteinModelPortali P0A507.
SMRi P0A507. Positions 45-340.
ModBasei Search...

Protein-protein interaction databases

STRINGi 233413.Mb1615.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAD96283 ; CAD96283 ; Mb1615 .
GeneIDi 1092504.
KEGGi mbo:Mb1615.
PATRICi 18005233. VBIMycBov88188_1764.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239958.
KOi K01012.
OMAi ETHHNID.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, sequencing, and identification of Mycobacterium bovis BCG biotin biosynthetic genes by complementing two Mycobacterium smegmatis biotin mutants."
    Yu S., Jacobs W.R. Jr.
    Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: BCG / Pasteur.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-935 / AF2122/97.

Entry informationi

Entry nameiBIOB_MYCBO
AccessioniPrimary (citable) accession number: P0A507
Secondary accession number(s): O06601, X2BIA2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: June 11, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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