ID   AK_MYCBO                Reviewed;         421 AA.
AC   P0A4Z9; O69676; P47731; P97048; P97181;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2005, sequence version 1.
DT   16-JUN-2009, entry version 29.
DE   RecName: Full=Aspartokinase;
DE            EC=2.7.2.4;
DE   AltName: Full=Aspartate kinase;
DE            Short=ASK;
GN   Name=ask; OrderedLocusNames=Mb3736c;
OS   Mycobacterium bovis.
OC   Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC   Corynebacterineae; Mycobacteriaceae; Mycobacterium;
OC   Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=1765;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   MEDLINE=22709107; PubMed=12788972; DOI=10.1073/pnas.1130426100;
RA   Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H.,
RA   Pryor M., Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S.,
RA   Harris B., Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R.,
RA   Parkhill J., Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G.;
RT   "The complete genome sequence of Mycobacterium bovis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 297-421.
RC   STRAIN=BCG / Pasteur;
RX   MEDLINE=94254720; PubMed=7910936;
RX   DOI=10.1111/j.1365-2958.1994.tb00342.x;
RA   Cirillo J.D., Weisbrod T.R., Pascopella L., Bloom B.R.,
RA   Jacobs W.R. Jr.;
RT   "Isolation and characterization of the aspartokinase and aspartate
RT   semialdehyde dehydrogenase operon from mycobacteria.";
RL   Mol. Microbiol. 11:629-639(1994).
CC   -!- CATALYTIC ACTIVITY: ATP + L-aspartate = ADP + 4-phospho-L-
CC       aspartate.
CC   -!- ENZYME REGULATION: Feedback inhibition by lysine and threonine (By
CC       similarity).
CC   -!- PATHWAY: Amino-acid biosynthesis; L-lysine biosynthesis via DAP
CC       pathway; tetrahydrodipicolinate from L-aspartate: step 1/4.
CC   -!- PATHWAY: Amino-acid biosynthesis; L-methionine biosynthesis via de
CC       novo pathway; L-homoserine from L-aspartate: step 1/3.
CC   -!- PATHWAY: Amino-acid biosynthesis; L-threonine biosynthesis; L-
CC       threonine from L-aspartate: step 1/5.
CC   -!- SUBUNIT: Tetramer consisting of 2 isoforms Alpha (catalytic) and 2
CC       isoforms Beta (function not known) (By similarity).
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative initiation; Named isoforms=2;
CC       Name=Alpha; Synonyms=Aspartokinase subunit alpha;
CC         IsoId=P0A4Z9-1; Sequence=Displayed;
CC       Name=Beta; Synonyms=Aspartokinase subunit beta;
CC         IsoId=P0A4Z9-2; Sequence=VSP_018661;
CC         Note=No experimental confirmation available;
CC   -!- SIMILARITY: Belongs to the aspartokinase family.
CC   -!- SIMILARITY: Contains 2 ACT domains.
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DR   EMBL; BX248347; CAD95922.1; -; Genomic_DNA.
DR   EMBL; Z18290; CAA79160.1; -; Genomic_DNA.
DR   PIR; S42425; S42425.
DR   RefSeq; NP_857374.1; -.
DR   GeneID; 1093392; -.
DR   GenomeReviews; BX248333_GR; Mb3736c.
DR   KEGG; mbo:Mb3736c; -.
DR   HOGENOM; P0A4Z9; -.
DR   OMA; P0A4Z9; MGKTTDN.
DR   BRENDA; 2.7.2.4; 3091.
DR   GO; GO:0016597; F:amino acid binding; IEA:InterPro.
DR   GO; GO:0004072; F:aspartate kinase activity; IEA:EC.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0019877; P:diaminopimelate biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR002912; ACT_bd.
DR   InterPro; IPR001048; Asp/Glu/Uridylate_kinase.
DR   InterPro; IPR005260; Asp_kin_monofn.
DR   InterPro; IPR001341; Asp_kin_reg.
DR   InterPro; IPR018042; Aspartate_kinase_CS.
DR   Gene3D; G3DSA:3.40.1160.10; Aa_kinase; 1.
DR   Pfam; PF00696; AA_kinase; 1.
DR   Pfam; PF01842; ACT; 2.
DR   TIGRFAMs; TIGR00656; asp_kin_monofn; 1.
DR   TIGRFAMs; TIGR00657; asp_kinases; 1.
DR   PROSITE; PS00324; ASPARTOKINASE; 1.
PE   3: Inferred from homology;
KW   Alternative initiation; Amino-acid biosynthesis; ATP-binding;
KW   Complete proteome; Diaminopimelate biosynthesis; Kinase;
KW   Lysine biosynthesis; Nucleotide-binding; Repeat; Transferase.
FT   CHAIN         1    421       Aspartokinase.
FT                                /FTId=PRO_0000002381.
FT   DOMAIN      266    338       ACT 1.
FT   DOMAIN      348    413       ACT 2.
FT   VAR_SEQ       1    249       Missing (in isoform Beta).
FT                                /FTId=VSP_018661.
SQ   SEQUENCE   421 AA;  44462 MW;  0567323B0D2085A7 CRC64;
     MALVVQKYGG SSVADAERIR RVAERIVATK KQGNDVVVVV SAMGDTTDDL LDLAQQVCPA
     PPPRELDMLL TAGERISNAL VAMAIESLGA HARSFTGSQA GVITTGTHGN AKIIDVTPGR
     LQTALEEGRV VLVAGFQGVS QDTKDVTTLG RGGSDTTAVA MAAALGADVC EIYTDVDGIF
     SADPRIVRNA RKLDTVTFEE MLEMAACGAK VLMLRCVEYA RRHNIPVHVR SSYSDRPGTV
     VVGSIKDVPM EDPILTGVAH DRSEAKVTIV GLPDIPGYAA KVFRAVADAD VNIDMVLQNV
     SKVEDGKTDI TFTCSRDVGP AAVEKLDSLR NEIGFSQLLY DDHIGKVSLI GAGMRSHPGV
     TATFCEALAA VGVNIELIST SEIRISVLCR DTELDKAVVA LHEAFGLGGD EEATVYAGTG
     R
//