Reviewed, UniProtKB/Swiss-Prot P0A4Z8 (AK_MYCTU)

Names and origin

Protein names

Recommended name:
    Aspartokinase
    EC=2.7.2.4
Alternative name(s):
    Aspartate kinase
      Short name=ASK

Gene names

Name:	ask
Ordered Locus Names:	Rv3709c, MT3812
ORF Names:	MTV025.057c

Organism

Mycobacterium tuberculosis [Complete proteome] [HAMAP]

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	421 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-aspartate = ADP + 4-phospho-L-aspartate.
Enzyme regulation	Feedback inhibition by lysine and threonine By similarity.
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; tetrahydrodipicolinate from L-aspartate: step 1/4. Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 1/3. Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 1/5.
Subunit structure	Tetramer consisting of 2 isoforms Alpha (catalytic) and 2 isoforms Beta (function not known) By similarity.
Sequence similarities	Belongs to the aspartokinase family. Contains 2 ACT domains.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Coding sequence diversity	Alternative initiation
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW amino acid binding Inferred from electronic annotation. Source: InterPro aspartate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]

Isoform Alpha (identifier: P0A4Z8-1)

Also known as: Aspartokinase subunit alpha;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Beta (identifier: P0A4Z8-2)

Also known as: Aspartokinase subunit beta;

The sequence of this isoform differs from the canonical sequence as follows:
     1-249: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 421	421	Aspartokinase		PRO_0000002385
Regions
	Domain	266 – 338	73	ACT 1
	Domain	348 – 413	66	ACT 2
Natural variations
	Alternative sequence	1 – 249	249	Missing in isoform Beta.		VSP_018663
Experimental info
	Sequence conflict	288 – 336	49	DADVN…EIGFS → RRRRQHRHGAAERLQGRGRQ DRHHLHLLPQTSGPPPWKNW TRSETRSAST in AAB49995. Ref.1
	Sequence conflict	383 – 421	39	IRISV…AGTGR → DQRSRCCAATPNWTRPWSRC MKRSGSAATRRPRCTRGRDG RWACQ in AAB49995. Ref.1
	Sequence conflict	383 – 421	39	IRISV…AGTGR → DQRSRCCAATPNWTRPWSRC MKRSGSAATRRPRCTRGRDG RWACQ in AAB49994. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform Alpha (Aspartokinase subunit alpha) [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 0567323B0D2085A7	FASTA	421	44,462
	10 20 30 40 50 60 MALVVQKYGG SSVADAERIR RVAERIVATK KQGNDVVVVV SAMGDTTDDL LDLAQQVCPA 70 80 90 100 110 120 PPPRELDMLL TAGERISNAL VAMAIESLGA HARSFTGSQA GVITTGTHGN AKIIDVTPGR 130 140 150 160 170 180 LQTALEEGRV VLVAGFQGVS QDTKDVTTLG RGGSDTTAVA MAAALGADVC EIYTDVDGIF 190 200 210 220 230 240 SADPRIVRNA RKLDTVTFEE MLEMAACGAK VLMLRCVEYA RRHNIPVHVR SSYSDRPGTV 250 260 270 280 290 300 VVGSIKDVPM EDPILTGVAH DRSEAKVTIV GLPDIPGYAA KVFRAVADAD VNIDMVLQNV 310 320 330 340 350 360 SKVEDGKTDI TFTCSRDVGP AAVEKLDSLR NEIGFSQLLY DDHIGKVSLI GAGMRSHPGV 370 380 390 400 410 420 TATFCEALAA VGVNIELIST SEIRISVLCR DTELDKAVVA LHEAFGLGGD EEATVYAGTG R
	Isoform Beta (Aspartokinase subunit beta). Checksum: 03C1BED54A968DDF	FASTA	172	18,188
	10 20 30 40 50 60 MEDPILTGVA HDRSEAKVTI VGLPDIPGYA AKVFRAVADA DVNIDMVLQN VSKVEDGKTD 70 80 90 100 110 120 ITFTCSRDVG PAAVEKLDSL RNEIGFSQLL YDDHIGKVSL IGAGMRSHPG VTATFCEALA 130 140 150 160 170 AVGVNIELIS TSEIRISVLC RDTELDKAVV ALHEAFGLGG DEEATVYAGT GR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mycobacterium tuberculosis ask-alpha, ask-beta and asd genes." Gilker J.M., Jucker M.T. Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[2]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[3]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U90239 Genomic DNA. Translation: AAB49995.1. U90239 Genomic DNA. Translation: AAB49994.1. BX842583 Genomic DNA. Translation: CAA18031.1. AE000516 Genomic DNA. Translation: AAK48180.1.
PIR	F70794.
RefSeq	NP_218226.1. NP_338366.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	885223. 926450.
GenomeReviews	Gene locus MT3812 in contig AE000516_GR. Gene locus Rv3709c in contig AL123456_GR.
KEGG	mtc:MT3812. mtu:Rv3709c.
TIGR	MT3812.
Organism-specific databases
TubercuList	Rv3709c.
Phylogenomic databases
HOGENOM	P0A4Z8.
OMA	P0A4Z8. MGKTTDN.
Enzyme and pathway databases
BRENDA	2.7.2.4. 809.
Family and domain databases
InterPro	IPR002912. ACT_bd. IPR001048. Asp/Glu/Uridylate_kinase. IPR005260. Asp_kin_monofn. IPR001341. Asp_kin_reg. IPR018042. Aspartate_kinase_CS. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
Pfam	PF00696. AA_kinase. 1 hit. PF01842. ACT. 2 hits. [Graphical view]
TIGRFAMs	TIGR00656. asp_kin_monofn. 1 hit. TIGR00657. asp_kinases. 1 hit.
PROSITE	PS00324. ASPARTOKINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AK_MYCTU

Accession

Primary (citable) accession number: P0A4Z8
Secondary accession number(s): O69676 , P47731, P97048, P97181

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	June 16, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)