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Reviewed, UniProtKB/Swiss-Prot P0A4Y6 (ARGB_MYCTU)

Last modified February 9, 2010. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Acetylglutamate kinase
    EC=2.7.2.8
Alternative name(s):
    NAG kinase
      Short name=AGK
    N-acetyl-L-glutamate 5-phosphotransferase
Gene names
Name: argB
Ordered Locus Names: Rv1654, MT1692
ORF Names: MTCY06H11.19
OrganismMycobacterium tuberculosis [Complete proteome] [HAMAP]
Taxonomic identifier1773 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

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Protein attributes

Sequence length294 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Catalytic activity

ATP + N-acetyl-L-glutamate = ADP + N-acetyl-L-glutamate 5-phosphate. HAMAP MF_00082

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 2/4. HAMAP MF_00082

Subunit structure

Homohexamer. Ref.3

Subcellular location

Cytoplasm Probable HAMAP MF_00082.

Sequence similarities

Belongs to the acetylglutamate kinase family.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 294294Acetylglutamate kinase HAMAP MF_00082
PRO_0000112637

Regions

Region69 – 702Substrate binding By similarity

Sites

Binding site911Substrate By similarity
Binding site1901Substrate By similarity
Site341Transition state stabilizer By similarity
Site2511Transition state stabilizer By similarity

Experimental info

Sequence conflict2461S → L in AAK45961. Ref.2

Secondary structure

1 ........................................................ 294
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
P0A4Y6-1 [UniParc].

Last modified March 15, 2005. Version 1.
Checksum: 5EFA44E9E3E8CFC7

FASTA29430,937
        10         20         30         40         50         60 
MSRIEALPTH IKAQVLAEAL PWLKQLHGKV VVVKYGGNAM TDDTLRRAFA ADMAFLRNCG 

        70         80         90        100        110        120 
IHPVVVHGGG PQITAMLRRL GIEGDFKGGF RVTTPEVLDV ARMVLFGQVG RELVNLINAH 

       130        140        150        160        170        180 
GPYAVGITGE DAQLFTAVRR SVTVDGVATD IGLVGDVDQV NTAAMLDLVA AGRIPVVSTL 

       190        200        210        220        230        240 
APDADGVVHN INADTAAAAV AEALGAEKLL MLTDIDGLYT RWPDRDSLVS EIDTGTLAQL 

       250        260        270        280        290 
LPTLESGMVP KVEACLRAVI GGVPSAHIID GRVTHCVLVE LFTDAGTGTK VVRG

« Hide

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References

« Hide 'large scale' references
[1]"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence."
Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. expand/collapse author list , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G.
Nature 393:537-544(1998) [PubMed: 9634230] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 25618 / H37Rv.
[2]"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains."
Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. expand/collapse author list , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.
J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CDC 1551 / Oshkosh.
[3]"Crystal structure of acetylglutamate kinase from Mycobacterium tuberculosis CDC1551."
New York structural genomics research consortium (NYSGRC)
Submitted (AUG-2005) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (2.80 ANGSTROMS) OF 4-294, SUBUNIT.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BX842577 Genomic DNA. Translation: CAB06648.1.
AE000516 Genomic DNA. Translation: AAK45961.1.
PIRA70621.
RefSeqNP_216170.1.
NP_336147.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2AP9X-ray2.80A/B/C/D/E/F4-294[»]
ModBaseSearch...

Genome annotation databases

GeneID888076.
924324.
GenomeReviewsGene locus MT1692 in contig AE000516_GR.
KEGGmtc:MT1692.
mtu:Rv1654.
TIGRMT1692.

Organism-specific databases

TubercuListRv1654.

Phylogenomic databases

HOGENOMHBG497643.
OMAMDIVEMV.

Enzyme and pathway databases

BRENDA2.7.2.8. 809.

Family and domain databases

HAMAPMF_00082_B. ArgB_B.
[Tree]
InterProIPR004662. AcgluKinase.
IPR001048. Asp/Glu/Uridylate_kinase.
IPR011148. GlcNAc_kinase.
IPR001057. Glu_5kinase.
[Graphical view]
Gene3DG3DSA:3.40.1160.10. Aa_kinase. 1 hit.
PfamPF00696. AA_kinase. 1 hit.
[Graphical view]
PIRSFPIRSF000728. NAGK. 1 hit.
PRINTSPR00474. GLU5KINASE.
TIGRFAMsTIGR00761. argB. 1 hit.
ProtoNetSearch...

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Entry information

Entry nameARGB_MYCTU
AccessionPrimary (citable) accession number: P0A4Y6
Secondary accession number(s): P94989
Entry history
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: February 9, 2010
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents