P0A4Y1 (CYA1_MYCBO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Adenylate cyclase
EC=4.6.1.1

Alternative name(s):
ATP pyrophosphate-lyase
Adenylyl cyclase

Gene names

Name:	cya
Ordered Locus Names:	Mb1651c

Organism

Mycobacterium bovis [Complete proteome] [HAMAP]

Taxonomic identifier

1765 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	443 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP = 3',5'-cyclic AMP + diphosphate.
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the adenylyl cyclase class-4/guanylyl cyclase family. Contains 1 guanylate cyclase domain.
Sequence caution	The sequence CAD96319.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	cAMP biosynthesis
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	intracellular signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW adenylate cyclase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 443	443	Adenylate cyclase		PRO_0000195726
Regions
	Transmembrane	47 – 69	23	Helical; Potential
	Transmembrane	74 – 93	20	Helical; Potential
	Transmembrane	98 – 120	23	Helical; Potential
	Transmembrane	124 – 143	20	Helical; Potential
	Transmembrane	148 – 167	20	Helical; Potential
	Transmembrane	180 – 202	23	Helical; Potential
	Topological domain	203 – 443	241	Cytoplasmic Potential
	Domain	251 – 378	128	Guanylate cyclase
Sites
	Metal binding	256	1	Magnesium By similarity
	Metal binding	300	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A4Y1 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 64B13168B4B792E2	FASTA	443	47,370
	10 20 30 40 50 60 MAARKCGAPP IAADGSTRRP DCVTAVRTQA RAPTQHYAES VARRQRVLTI TAWLAVVVTG 70 80 90 100 110 120 SFALMQLATG AGGWYIALIN VFTAVTFAIV PLLHRFGGLV APLTFIGTAY VAIFAIGWDV 130 140 150 160 170 180 GTDAGAQFFF LVAAALVVLL VGIEHTALAV GLAAVAAGLV IALEFLVPPD TGLQPPWAMS 190 200 210 220 230 240 VSFVLTTVSA CGVAVATVWF ALRDTARAEA VMEAEHDRSE ALLANMLPAS IAERLKEPER 250 260 270 280 290 300 NIIADKYDEA SVLFADIVGF TERASSTAPA DLVRFLDRLY SAFDELVDQH GLEKIKVSGD 310 320 330 340 350 360 SYMVVSGVPR PRPDHTQALA DFALDMTNVA AQLKDPRGNP VPLRVGLATG PVVAGVVGSR 370 380 390 400 410 420 RFFYDVWGDA VNVASRMEST DSVGQIQVPD EVYERLKDDF VLRERGHINV KGKGVMRTWY 430 440 LIGRKVAADP GEVRGAEPRT AGV

References

[1]

"The complete genome sequence of Mycobacterium bovis." Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M., Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B., Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J. , Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G. Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003) [PubMed: 12788972] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-935 / AF2122/97.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX248339 Genomic DNA. Translation: CAD96319.1. Different initiation.
RefSeq	NP_855304.1. NC_002945.3.
3D structure databases
ProteinModelPortal	P0A4Y1.
SMR	P0A4Y1. Positions 245-428.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000017247; EBMYCP00000017082; EBMYCG00000017242.
GeneID	1092586.
GenomeReviews	Gene locus Mb1651c in contig BX248333_GR.
KEGG	mbo:Mb1651c.
PATRIC	18005309. VBIMycBov88188_1802.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000015402.
HOGENOM	HBG564705.
ProtClustDB	CLSK799821.
Enzyme and pathway databases
BioCyc	MBOV233413:MB1651C-MONOMER.
Family and domain databases
InterPro	IPR001054. A/G_cyclase. IPR018297. A/G_cyclase_CS. [Graphical view]
Gene3D	G3DSA:3.30.70.1230. A/G_cyclase. 1 hit.
KO	K01768.
Pfam	PF00211. Guanylate_cyc. 1 hit. [Graphical view]
SMART	SM00044. CYCc. 1 hit. [Graphical view]
SUPFAM	SSF55073. A/G_cyclase. 1 hit.
PROSITE	PS00452. GUANYLATE_CYCLASE_1. 1 hit. PS50125. GUANYLATE_CYCLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYA1_MYCBO

Accession

Primary (citable) accession number: P0A4Y1
Secondary accession number(s): O06142, O30820

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program