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Reviewed, UniProtKB/Swiss-Prot P0A4X2 (ALR_MYCTU)

Last modified November 3, 2009. Version 39. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Alanine racemase
    EC=5.1.1.1
Gene names
Name: alr
Ordered Locus Names: Rv3423c, MT3532
ORF Names: MTCY78.06
OrganismMycobacterium tuberculosis [Complete proteome] [HAMAP]
Taxonomic identifier1773 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

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Protein attributes

Sequence length386 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Provides the D-alanine required for cell wall biosynthesis. HAMAP MF_01201

Catalytic activity

L-alanine = D-alanine. HAMAP MF_01201

Cofactor

Pyridoxal phosphate By similarity.

Pathway

Amino-acid biosynthesis; D-alanine biosynthesis; D-alanine from L-alanine: step 1/1. HAMAP MF_01201

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_01201

Sequence similarities

Belongs to the alanine racemase family.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 386386Alanine racemase HAMAP MF_01201
PRO_0000114539

Sites

Active site441Proton acceptor; specific for D-alanine By similarity
Active site2731Proton acceptor; specific for L-alanine By similarity

Amino acid modifications

Modified residue441N6-(pyridoxal phosphate)lysine By similarity

Secondary structure

1 ......................................................................... 386
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
P0A4X2-1 [UniParc].

Last modified March 15, 2005. Version 1.
Checksum: 806225696ACB0BFE

FASTA38640,926
        10         20         30         40         50         60 
MAMTPISQTP GLLAEAMVDL GAIEHNVRVL REHAGHAQLM AVVKADGYGH GATRVAQTAL 

        70         80         90        100        110        120 
GAGAAELGVA TVDEALALRA DGITAPVLAW LHPPGIDFGP ALLADVQVAV SSLRQLDELL 

       130        140        150        160        170        180 
HAVRRTGRTA TVTVKVDTGL NRNGVGPAQF PAMLTALRQA MAEDAVRLRG LMSHMVYADK 

       190        200        210        220        230        240 
PDDSINDVQA QRFTAFLAQA REQGVRFEVA HLSNSSATMA RPDLTFDLVR PGIAVYGLSP 

       250        260        270        280        290        300 
VPALGDMGLV PAMTVKCAVA LVKSIRAGEG VSYGHTWIAP RDTNLALLPI GYADGVFRSL 

       310        320        330        340        350        360 
GGRLEVLING RRCPGVGRIC MDQFMVDLGP GPLDVAEGDE AILFGPGIRG EPTAQDWADL 

       370        380 
VGTIHYEVVT SPRGRITRTY REAENR

« Hide

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References

« Hide 'large scale' references
[1]"Characterization of the alanine racemases from two Mycobacteria."
Strych U., Penland R.L., Jimenez M., Krause K.L., Benedik M.J.
FEMS Microbiol. Lett. 196:93-98(2001) [PubMed: 11267762] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence."
Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. expand/collapse author list , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G.
Nature 393:537-544(1998) [PubMed: 9634230] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 25618 / H37Rv.
[3]"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains."
Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. expand/collapse author list , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.
J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CDC 1551 / Oshkosh.
+Additional computationally mapped references.

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Cross-references

Sequence databases

AF172731 Genomic DNA. Translation: AAD51033.1. Different initiation.
BX842583 Genomic DNA. Translation: CAB01033.1. Different initiation.
AE000516 Genomic DNA. Translation: AAK47870.1. Different initiation.
PIRD70738.
RefSeqNP_217940.1.
NP_338056.1.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1XFCX-ray1.90A/B3-386[»]
ModBaseSearch...

Genome annotation databases

GeneID887634.
923425.
GenomeReviewsGene locus MT3532 in contig AE000516_GR.
Gene locus Rv3423c in contig AL123456_GR.
KEGGmtc:MT3532.
mtu:Rv3423c.
TIGRMT3532.

Organism-specific databases

TubercuListRv3423c.

Phylogenomic databases

HOGENOMP0A4X2.
OMAPTHERSI.

Enzyme and pathway databases

BRENDA5.1.1.1. 809.

Family and domain databases

HAMAPMF_01201.
[Tree]
InterProIPR011079. Ala_racemase_C.
IPR001608. Ala_racemase_N.
IPR000821. Ala_racemase_reg.
[Graphical view]
PfamPF00842. Ala_racemase_C. 1 hit.
PF01168. Ala_racemase_N. 1 hit.
[Graphical view]
PRINTSPR00992. ALARACEMASE.
TIGRFAMsTIGR00492. alr. 1 hit.
PROSITEPS00395. ALANINE_RACEMASE. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameALR_MYCTU
AccessionPrimary (citable) accession number: P0A4X2
Secondary accession number(s): Q50705
Entry history
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: November 3, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents