Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P0A4U6 (NTCA_NOSS1) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Global nitrogen regulator
Alternative name(s):
DNA-binding protein VF1
Nitrogen-responsive regulatory protein
Gene names
Name:ntcA
Synonyms:bifA
Ordered Locus Names:alr4392
OrganismNostoc sp. (strain PCC 7120 / UTEX 2576) [Complete proteome] [HAMAP]
Taxonomic identifier103690 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaNostocalesNostocaceaeNostoc

Protein attributes

Sequence length223 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for full expression of proteins subject to ammonium repression. Transcriptional activator of genes subject to nitrogen control.

Has affinity for the xisA upstream region. Binds to a 66 bp region containing three repeats of the consensus recognition sequence 5'-ACATT-3'.

Sequence similarities

Contains 1 cyclic nucleotide-binding domain.

Contains 1 HTH crp-type DNA-binding domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   LigandDNA-binding
   Molecular functionActivator
   Technical term3D-structure
Complete proteome
Gene Ontology (GO)
   Biological_processtranscription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentintracellular

Inferred from electronic annotation. Source: InterPro

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

sequence-specific DNA binding transcription factor activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 223223Global nitrogen regulator
PRO_0000100184

Regions

Domain143 – 21674HTH crp-type
DNA binding176 – 19520H-T-H motif By similarity

Secondary structure

........................................ 223
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P0A4U6 [UniParc].

Last modified March 15, 2005. Version 1.
Checksum: 07FCE891766E7021

FASTA22324,918
        10         20         30         40         50         60 
MIVTQDKALA NVFRQMATGA FPPVVETFER NKTIFFPGDP AERVYFLLKG AVKLSRVYEA 

        70         80         90        100        110        120 
GEEITVALLR ENSVFGVLSL LTGNKSDRFY HAVAFTPVEL LSAPIEQVEQ ALKENPELSM 

       130        140        150        160        170        180 
LMLRGLSSRI LQTEMMIETL AHRDMGSRLV SFLLILCRDF GVPCADGITI DLKLSHQAIA 

       190        200        210        220 
EAIGSTRVTV TRLLGDLREK KMISIHKKKI TVHKPVTLSR QFT 

« Hide

References

« Hide 'large scale' references
[1]"Anabaena sp. strain PCC 7120 bifA gene encoding a sequence-specific DNA-binding protein cloned by in vivo transcriptional interference selection."
Wei T.-F., Ramasubramanian T.S., Pu F., Golden J.W.
J. Bacteriol. 175:4025-4035(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"General distribution of the nitrogen control gene ntcA in cyanobacteria."
Frias J.E., Merida A., Herrero A., Martin-Nieto J.M., Flores E.
J. Bacteriol. 175:5710-5713(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120."
Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. expand/collapse author list , Takazawa M., Yamada M., Yasuda M., Tabata S.
DNA Res. 8:205-213(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PCC 7120 / UTEX 2576.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L10036 Genomic DNA. Translation: AAD04183.1.
X71608 Genomic DNA. Translation: CAA50608.1.
BA000019 Genomic DNA. Translation: BAB76091.1.
PIRAH2354.
B48640.
RefSeqNP_488432.1. NC_003272.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3LA2X-ray2.60A/B1-223[»]
3LA3X-ray2.40A/B1-223[»]
3LA7X-ray1.90A/B1-223[»]
ProteinModelPortalP0A4U6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-59350N.
STRING103690.alr4392.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB76091; BAB76091; BAB76091.
GeneID1107993.
KEGGana:alr4392.
PATRIC22779368. VBINosSp37423_5131.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0664.
HOGENOMHOG000232503.
OMAYHSVAFT.
OrthoDBEOG6QRWC5.

Family and domain databases

Gene3D1.10.10.10. 1 hit.
2.60.120.10. 1 hit.
InterProIPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR012318. HTH_CRP_2.
IPR014710. RmlC-like_jellyroll.
IPR001808. Tscrpt_reg_HTH_Crp.
IPR018335. Tscrpt_reg_HTH_Crp-type_CS.
IPR022299. Tscrpt_reg_NtcA.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamPF00027. cNMP_binding. 1 hit.
PF00325. Crp. 1 hit.
[Graphical view]
PRINTSPR00034. HTHCRP.
SMARTSM00100. cNMP. 1 hit.
SM00419. HTH_CRP. 1 hit.
[Graphical view]
SUPFAMSSF51206. SSF51206. 1 hit.
TIGRFAMsTIGR03697. NtcA_cyano. 1 hit.
PROSITEPS50042. CNMP_BINDING_3. 1 hit.
PS00042. HTH_CRP_1. 1 hit.
PS51063. HTH_CRP_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP0A4U6.

Entry information

Entry nameNTCA_NOSS1
AccessionPrimary (citable) accession number: P0A4U6
Secondary accession number(s): Q05061
Entry history
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: May 14, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references