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P0A4U6

- NTCA_NOSS1

UniProt

P0A4U6 - NTCA_NOSS1

Protein

Global nitrogen regulator

Gene

ntcA

Organism
Nostoc sp. (strain PCC 7120 / UTEX 2576)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 60 (01 Oct 2014)
      Sequence version 1 (15 Mar 2005)
      Previous versions | rss
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    Functioni

    Required for full expression of proteins subject to ammonium repression. Transcriptional activator of genes subject to nitrogen control.
    Has affinity for the xisA upstream region. Binds to a 66 bp region containing three repeats of the consensus recognition sequence 5'-ACATT-3'.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi176 – 19520H-T-H motifPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. sequence-specific DNA binding transcription factor activity Source: InterPro

    GO - Biological processi

    1. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Global nitrogen regulator
    Alternative name(s):
    DNA-binding protein VF1
    Nitrogen-responsive regulatory protein
    Gene namesi
    Name:ntcA
    Synonyms:bifA
    Ordered Locus Names:alr4392
    OrganismiNostoc sp. (strain PCC 7120 / UTEX 2576)
    Taxonomic identifieri103690 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaNostocalesNostocaceaeNostoc
    ProteomesiUP000002483: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. intracellular Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 223223Global nitrogen regulatorPRO_0000100184Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    DIPiDIP-59350N.
    STRINGi103690.alr4392.

    Structurei

    Secondary structure

    1
    223
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi7 – 1610
    Beta strandi24 – 285
    Beta strandi33 – 353
    Beta strandi43 – 508
    Beta strandi52 – 576
    Beta strandi63 – 697
    Beta strandi74 – 763
    Helixi78 – 814
    Helixi83 – 853
    Beta strandi90 – 10415
    Helixi105 – 1128
    Helixi116 – 14126
    Helixi145 – 16016
    Beta strandi161 – 1633
    Beta strandi165 – 1706
    Helixi176 – 1838
    Helixi187 – 19913
    Beta strandi202 – 2065
    Beta strandi209 – 2124
    Helixi215 – 2184
    Turni219 – 2213

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3LA2X-ray2.60A/B1-223[»]
    3LA3X-ray2.40A/B1-223[»]
    3LA7X-ray1.90A/B1-223[»]
    ProteinModelPortaliP0A4U6.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0A4U6.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini143 – 21674HTH crp-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 cyclic nucleotide-binding domain.PROSITE-ProRule annotation
    Contains 1 HTH crp-type DNA-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0664.
    HOGENOMiHOG000232503.
    OMAiYHSVAFT.
    OrthoDBiEOG6QRWC5.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    2.60.120.10. 1 hit.
    InterProiIPR018490. cNMP-bd-like.
    IPR000595. cNMP-bd_dom.
    IPR012318. HTH_CRP_2.
    IPR014710. RmlC-like_jellyroll.
    IPR001808. Tscrpt_reg_HTH_Crp.
    IPR018335. Tscrpt_reg_HTH_Crp-type_CS.
    IPR022299. Tscrpt_reg_NtcA.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF00027. cNMP_binding. 1 hit.
    PF00325. Crp. 1 hit.
    [Graphical view]
    PRINTSiPR00034. HTHCRP.
    SMARTiSM00100. cNMP. 1 hit.
    SM00419. HTH_CRP. 1 hit.
    [Graphical view]
    SUPFAMiSSF51206. SSF51206. 1 hit.
    TIGRFAMsiTIGR03697. NtcA_cyano. 1 hit.
    PROSITEiPS50042. CNMP_BINDING_3. 1 hit.
    PS00042. HTH_CRP_1. 1 hit.
    PS51063. HTH_CRP_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0A4U6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIVTQDKALA NVFRQMATGA FPPVVETFER NKTIFFPGDP AERVYFLLKG    50
    AVKLSRVYEA GEEITVALLR ENSVFGVLSL LTGNKSDRFY HAVAFTPVEL 100
    LSAPIEQVEQ ALKENPELSM LMLRGLSSRI LQTEMMIETL AHRDMGSRLV 150
    SFLLILCRDF GVPCADGITI DLKLSHQAIA EAIGSTRVTV TRLLGDLREK 200
    KMISIHKKKI TVHKPVTLSR QFT 223
    Length:223
    Mass (Da):24,918
    Last modified:March 15, 2005 - v1
    Checksum:i07FCE891766E7021
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L10036 Genomic DNA. Translation: AAD04183.1.
    X71608 Genomic DNA. Translation: CAA50608.1.
    BA000019 Genomic DNA. Translation: BAB76091.1.
    PIRiAH2354.
    B48640.
    RefSeqiNP_488432.1. NC_003272.1.

    Genome annotation databases

    EnsemblBacteriaiBAB76091; BAB76091; BAB76091.
    GeneIDi1107993.
    KEGGiana:alr4392.
    PATRICi22779368. VBINosSp37423_5131.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L10036 Genomic DNA. Translation: AAD04183.1 .
    X71608 Genomic DNA. Translation: CAA50608.1 .
    BA000019 Genomic DNA. Translation: BAB76091.1 .
    PIRi AH2354.
    B48640.
    RefSeqi NP_488432.1. NC_003272.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3LA2 X-ray 2.60 A/B 1-223 [» ]
    3LA3 X-ray 2.40 A/B 1-223 [» ]
    3LA7 X-ray 1.90 A/B 1-223 [» ]
    ProteinModelPortali P0A4U6.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-59350N.
    STRINGi 103690.alr4392.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB76091 ; BAB76091 ; BAB76091 .
    GeneIDi 1107993.
    KEGGi ana:alr4392.
    PATRICi 22779368. VBINosSp37423_5131.

    Phylogenomic databases

    eggNOGi COG0664.
    HOGENOMi HOG000232503.
    OMAi YHSVAFT.
    OrthoDBi EOG6QRWC5.

    Miscellaneous databases

    EvolutionaryTracei P0A4U6.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    2.60.120.10. 1 hit.
    InterProi IPR018490. cNMP-bd-like.
    IPR000595. cNMP-bd_dom.
    IPR012318. HTH_CRP_2.
    IPR014710. RmlC-like_jellyroll.
    IPR001808. Tscrpt_reg_HTH_Crp.
    IPR018335. Tscrpt_reg_HTH_Crp-type_CS.
    IPR022299. Tscrpt_reg_NtcA.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF00027. cNMP_binding. 1 hit.
    PF00325. Crp. 1 hit.
    [Graphical view ]
    PRINTSi PR00034. HTHCRP.
    SMARTi SM00100. cNMP. 1 hit.
    SM00419. HTH_CRP. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51206. SSF51206. 1 hit.
    TIGRFAMsi TIGR03697. NtcA_cyano. 1 hit.
    PROSITEi PS50042. CNMP_BINDING_3. 1 hit.
    PS00042. HTH_CRP_1. 1 hit.
    PS51063. HTH_CRP_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Anabaena sp. strain PCC 7120 bifA gene encoding a sequence-specific DNA-binding protein cloned by in vivo transcriptional interference selection."
      Wei T.-F., Ramasubramanian T.S., Pu F., Golden J.W.
      J. Bacteriol. 175:4025-4035(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "General distribution of the nitrogen control gene ntcA in cyanobacteria."
      Frias J.E., Merida A., Herrero A., Martin-Nieto J.M., Flores E.
      J. Bacteriol. 175:5710-5713(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: PCC 7120 / UTEX 2576.

    Entry informationi

    Entry nameiNTCA_NOSS1
    AccessioniPrimary (citable) accession number: P0A4U6
    Secondary accession number(s): Q05061
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 15, 2005
    Last sequence update: March 15, 2005
    Last modified: October 1, 2014
    This is version 60 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3