P0A4U3 (CADC_LACLL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cadmium resistance transcriptional regulatory protein CadC

Alternative name(s):
Cadmium efflux system accessory protein

Gene names

Name:

cadC

Encoded on

Plasmid pND302 Ref.1
Plasmid pAH82 Ref.2

Organism

Lactococcus lactis subsp. lactis (Streptococcus lactis)

Taxonomic identifier

1360 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Lactococcus › Lactococcus lactis

Protein attributes

Sequence length	119 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Metal-binding repressor for the cad operon. Involved in resistance to heavy metals, such as cadmium, bismuth, zinc or lead. Metal binding causes the repressor to dissociate from the DNA By similarity.
Subunit structure	Homodimer By similarity.
Sequence similarities	Contains 1 HTH arsR-type DNA-binding domain.

Ontologies

Keywords
Biological process	Cadmium resistance Transcription Transcription regulation
Ligand	Cadmium DNA-binding Metal-binding
Technical term	Plasmid
Gene Ontology (GO)
Biological_process	response to cadmium ion Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 119	119	Cadmium resistance transcriptional regulatory protein CadC		PRO_0000160617
Regions
	Domain	23 – 118	96	HTH arsR-type
	DNA binding	58 – 77	20	H-T-H motif By similarity
Sites
	Metal binding	6	1	Cadmium; shared with dimeric partner By similarity
	Metal binding	10	1	Cadmium; shared with dimeric partner By similarity
	Metal binding	57	1	Cadmium; shared with dimeric partner By similarity
	Metal binding	59	1	Cadmium; shared with dimeric partner By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A4U3 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: B34C01C21DC2C944	FASTA	119	13,382
	10 20 30 40 50 60 MNNEICEITC IHEDKVNRAK SKLANFDTPS VSGFFKILSD ENRLKIVHAL VHEDELCVCD 70 80 90 100 110 IANIIDASVA TTSHHLNSLK KLGVVDSHKD GKLVYYFIKN IKILNLMELG VNFKEEVLA

References

[1]	"Genetic analysis of regions involved in replication and cadmium resistance of the plasmid pND302 from Lactococcus lactis." Liu C.-Q., Khunajakr N., Chia L.G., Deng Y.-M., Charoenchai P., Dunn N.W. Plasmid 38:79-90(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: M71.
[2]	"Novel type I restriction specificities through domain shuffling of HsdS subunits in Lactococcus lactis." O'Sullivan D., Twomey D.P., Coffey A., Hill C., Fitzgerald G.F., Ross P.R. Mol. Microbiol. 36:866-875(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DPC220.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U78967 Genomic DNA. Translation: AAB37344.1. AF243383 Genomic DNA. Translation: AAF98301.1.
RefSeq	NP_862601.1. NC_004966.1. YP_001174713.1. NC_009435.1. YP_001966470.1. NC_010901.1. YP_004761536.1. NC_015863.1.
3D structure databases
ProteinModelPortal	P0A4U3.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	11013250. 3125820. 5077334. 6385740.
Phylogenomic databases
HOGENOM	HOG000144506.
ProtClustDB	CLSK811323.
Family and domain databases
Gene3D	1.10.10.10. 1 hit.
InterPro	IPR018334. ArsR_HTH. IPR001845. HTH_ArsR_DNA-bd_dom. IPR011991. WHTH_DNA-bd_dom. [Graphical view]
Pfam	PF01022. HTH_5. 1 hit. [Graphical view]
PRINTS	PR00778. HTHARSR.
SMART	SM00418. HTH_ARSR. 1 hit. [Graphical view]
PROSITE	PS00846. HTH_ARSR_1. 1 hit. PS50987. HTH_ARSR_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CADC_LACLL

Accession

Primary (citable) accession number: P0A4U3
Secondary accession number(s): P94887

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	May 1, 2013
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program