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Protein

HTH-type transcriptional activator TipA

Gene

tipA

Organism
Streptomyces lividans
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional activator. Is activated when bound to the antibiotic thiostrepton.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi5 – 2420H-T-H motifPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
HTH-type transcriptional activator TipA
Gene namesi
Name:tipA
OrganismiStreptomyces lividans
Taxonomic identifieri1916 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 253253HTH-type transcriptional activator TipAPRO_0000013606Add
BLAST

Interactioni

Subunit structurei

Homodimer.Curated

Structurei

Secondary structure

1
253
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi5 – 128Combined sources
Helixi16 – 249Combined sources
Beta strandi31 – 333Combined sources
Beta strandi39 – 413Combined sources
Helixi43 – 5715Combined sources
Helixi62 – 698Combined sources
Helixi79 – 10527Combined sources
Helixi116 – 1238Combined sources
Helixi129 – 1313Combined sources
Helixi132 – 1387Combined sources
Helixi144 – 1529Combined sources
Helixi165 – 18016Combined sources
Helixi187 – 20317Combined sources
Helixi209 – 21810Combined sources
Helixi223 – 2297Combined sources
Helixi230 – 2323Combined sources
Beta strandi233 – 2353Combined sources
Helixi236 – 25116Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1NY9NMR-A111-253[»]
2MBZNMR-A110-253[»]
2MC0NMR-A110-253[»]
2VZ4X-ray2.90A2-109[»]
ProteinModelPortaliP0A4T9.
SMRiP0A4T9. Positions 160-253.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A4T9.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 7171HTH merR-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 HTH merR-type DNA-binding domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di1.10.490.50. 1 hit.
InterProiIPR009061. DNA-bd_dom_put.
IPR000551. MerR-type_HTH_dom.
IPR012925. TipAS_dom.
[Graphical view]
PfamiPF13411. MerR_1. 1 hit.
PF07739. TipAS. 1 hit.
[Graphical view]
PRINTSiPR00040. HTHMERR.
SMARTiSM00422. HTH_MERR. 1 hit.
[Graphical view]
SUPFAMiSSF46955. SSF46955. 1 hit.
SSF89082. SSF89082. 1 hit.
PROSITEiPS00552. HTH_MERR_1. 1 hit.
PS50937. HTH_MERR_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform Long (identifier: P0A4T9-1) [UniParc]FASTAAdd to basket

Also known as: TipA-L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSYSVGQVAG FAGVTVRTLH HYDDIGLLVP SERSHAGHRR YSDADLDRLQ
60 70 80 90 100
QILFYRELGF PLDEVAALLD DPAADPRAHL RRQHELLSAR IGKLQKMAAA
110 120 130 140 150
VEQAMEARSM GINLTPEEKF EVFGDFDPDQ YEEEVRERWG NTDAYRQSKE
160 170 180 190 200
KTASYTKEDW QRIQDEADEL TRRFVALMDA GEPADSEGAM DAAEDHRQGI
210 220 230 240 250
ARNHYDCGYE MHTCLGEMYV SDERFTRNID AAKPGLAAYM RDAILANAVR

HTP
Length:253
Mass (Da):28,711
Last modified:March 15, 2005 - v1
Checksum:i0B32F6191BF2B779
GO
Isoform Short (identifier: P0A4T9-2) [UniParc]FASTAAdd to basket

Also known as: TipA-S

The sequence of this isoform differs from the canonical sequence as follows:
     1-109: Missing.

Show »
Length:144
Mass (Da):16,563
Checksum:i11092740A7B10FDA
GO

Sequence cautioni

The sequence AAC13653.1 differs from that shown. Reason: Erroneous initiation. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 109109Missing in isoform Short. CuratedVSP_018752Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S64314 Genomic DNA. Translation: AAB27737.1.
M24524 Genomic DNA. Translation: AAC13653.1. Different initiation.
PIRiS35354.

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S64314 Genomic DNA. Translation: AAB27737.1.
M24524 Genomic DNA. Translation: AAC13653.1. Different initiation.
PIRiS35354.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1NY9NMR-A111-253[»]
2MBZNMR-A110-253[»]
2MC0NMR-A110-253[»]
2VZ4X-ray2.90A2-109[»]
ProteinModelPortaliP0A4T9.
SMRiP0A4T9. Positions 160-253.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP0A4T9.

Family and domain databases

Gene3Di1.10.490.50. 1 hit.
InterProiIPR009061. DNA-bd_dom_put.
IPR000551. MerR-type_HTH_dom.
IPR012925. TipAS_dom.
[Graphical view]
PfamiPF13411. MerR_1. 1 hit.
PF07739. TipAS. 1 hit.
[Graphical view]
PRINTSiPR00040. HTHMERR.
SMARTiSM00422. HTH_MERR. 1 hit.
[Graphical view]
SUPFAMiSSF46955. SSF46955. 1 hit.
SSF89082. SSF89082. 1 hit.
PROSITEiPS00552. HTH_MERR_1. 1 hit.
PS50937. HTH_MERR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Thiostrepton-induced gene expression in Streptomyces lividans."
    Murakami T., Holt T.G., Thompson C.J.
    J. Bacteriol. 171:1459-1466(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Autogenous transcriptional activation of a thiostrepton-induced gene in Streptomyces lividans."
    Holmes D.J., Caso J.L., Thompson C.J.
    EMBO J. 12:3183-3191(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE, CHARACTERIZATION.

Entry informationi

Entry nameiTIPA_STRLI
AccessioniPrimary (citable) accession number: P0A4T9
Secondary accession number(s): P32184
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: October 14, 2015
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.