P0A4S8 (LFTR_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Leucyl/phenylalanyl-tRNA--protein transferase
EC=2.3.2.6

Alternative name(s):
L/F-transferase
Leucyltransferase
Phenyalanyltransferase

Gene names

Name:	aat
Ordered Locus Names:	BR0905, BS1330_I0901

Organism

Brucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]

Taxonomic identifier

204722 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella suis

Protein attributes

Sequence length	204 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Functions in the N-end rule pathway of protein degradation where it conjugates Leu, Phe and, less efficiently, Met from aminoacyl-tRNAs to the N-termini of proteins containing an N-terminal arginine or lysine By similarity. HAMAP-Rule MF_00688
Catalytic activity	L-leucyl-tRNA + protein = tRNA + L-leucyl-protein. HAMAP-Rule MF_00688 L-phenylalanyl-tRNA + protein = tRNA + L-phenylalanyl-protein. HAMAP-Rule MF_00688
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the L/F-transferase family.
Sequence caution	The sequence AAN29833.1 differs from that shown. Reason: Frameshift at position 6. The sequence AEM18250.1 differs from that shown. Reason: Frameshift at position 6.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	protein catabolic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	leucyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 204	204	Leucyl/phenylalanyl-tRNA--protein transferase HAMAP-Rule MF_00688		PRO_0000207209

Sequences

	Sequence		Length	Mass (Da)
	P0A4S8 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 2B2155002265E0EC	FASTA	204	23,153
	10 20 30 40 50 60 MTAEAPPDDD IIEPEMLLRA YATGIFPMAE EADDPEVFWV RPEKRGVIPL DGFHIPRSLQ 70 80 90 100 110 120 KTIRQGIFEI RLDSNFAGVI EGCASGTGER ARTWINEPIR RAYAKLFEIG HCHTVEAWYE 130 140 150 160 170 180 GKLAGGLYGV TLGRAFFGES MFTRKRDASK VCLAYLVQHL SRQGFVLLDT QFTTPHLERF 190 200 GALEVPRKEY EEMLERALEG IARF

References

[1]

"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

[2]

"Revised genome sequence of Brucella suis 1330." Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R. J. Bacteriol. 193:6410-6410(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014291 Genomic DNA. Translation: AAN29833.1. Frameshift. CP002997 Genomic DNA. Translation: AEM18250.1. Frameshift.
RefSeq	NP_697918.1. NC_004310.3. YP_005615742.1. NC_017251.1.
3D structure databases
ProteinModelPortal	P0A4S8.
ModBase	Search...
Protein-protein interaction databases
STRING	204722.BR0905.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAN29833; AAN29833; BR0905. AEM18250; AEM18250; BS1330_I0901.
GeneID	1166574. 12137927.
KEGG	bms:BR0905. bsi:BS1330_I0901.
PATRIC	17790116. VBIBruSui107850_0918.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2360.
HOGENOM	HOG000102326.
KO	K00684.
ProtClustDB	PRK00301.
Family and domain databases
HAMAP	MF_00688. Leu_Phe_trans.
InterPro	IPR016181. Acyl_CoA_acyltransferase. IPR004616. Leu/Phe-tRNA_Trfase. [Graphical view]
Pfam	PF03588. Leu_Phe_trans. 1 hit. [Graphical view]
SUPFAM	SSF55729. Acyl_CoA_acyltransferase. 1 hit.
TIGRFAMs	TIGR00667. aat. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LFTR_BRUSU

Accession

Primary (citable) accession number: P0A4S8
Secondary accession number(s): G0K9D3, Q8G123, Q8YGU3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	May 1, 2013
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program