P0A4P1 (TRMD_STRCO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine-N(1)-)-methyltransferase
EC=2.1.1.228

Alternative name(s):
M1G-methyltransferase
tRNA [GM37] methyltransferase

Gene names

Name:	trmD
Ordered Locus Names:	SCO5594
ORF Names:	SC2E1.11

Organism

Streptomyces coelicolor

Taxonomic identifier

1902 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	277 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates guanosine-37 in various tRNAs By similarity. HAMAP MF_00605
Catalytic activity	S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA. HAMAP MF_00605
Subunit structure	Homodimer By similarity. HAMAP MF_00605
Subcellular location	Cytoplasm Potential HAMAP MF_00605.
Sequence similarities	Belongs to the RNA methyltransferase TrmD family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro tRNA (guanine-N1-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 277	277	tRNA (guanine-N(1)-)-methyltransferase HAMAP MF_00605		PRO_0000060477
Regions
	Region	140 – 145	6	S-adenosyl-L-methionine binding By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A4P1 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 61CB740770A3A4C8	FASTA	277	30,828
	10 20 30 40 50 60 MRLDVVTIFP EYLEPLNVSL VGKARARGQL GVHVHDLRDW TYDRHNTVDD TPYGGGPGMV 70 80 90 100 110 120 MKTEPWGDAL DSVLADGYET GCGEPALVVP TPSGRPFTQE LAVHLSERPW LIFTPARYEG 130 140 150 160 170 180 IDRRVVDEYA TRMPVYEVSI GDYVLAGGEA AVLVVTEAVA RLLPGVLGNA ESHRDDSFAP 190 200 210 220 230 240 GAMANLLEGP VHTKPPQWRG RGIPDVLLSG HHGKIARWRR DEALRRTTAN RPDLIERCDP 250 260 270 AAFDKKDREM LSILGWQPDP DGEPYGRFWR RTPGMEE

References

[1]

"Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2)." Bentley S.D., Chater K.F., Cerdeno-Tarraga A.-M., Challis G.L., Thomson N.R., James K.D., Harris D.E., Quail M.A., Kieser H., Harper D., Bateman A., Brown S., Chandra G., Chen C.W., Collins M., Cronin A., Fraser A., Goble A. , Hidalgo J., Hornsby T., Howarth S., Huang C.-H., Kieser T., Larke L., Murphy L.D., Oliver K., O'Neil S., Rabbinowitsch E., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Saunders D., Sharp S., Squares R., Squares S., Taylor K., Warren T., Wietzorrek A., Woodward J.R., Barrell B.G., Parkhill J., Hopwood D.A. Nature 417:141-147(2002) [PubMed: 12000953] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-471 / A3(2) / M145.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL939124 Genomic DNA. Translation: CAA19386.1.
PIR	T34779.
RefSeq	NP_629728.1. NC_003888.3.
3D structure databases
ProteinModelPortal	P0A4P1.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1101035.
GenomeReviews	Gene locus SCO5594 in contig AL645882_GR.
KEGG	sco:SCO5594.
NMPDR	fig|100226.1.peg.5547.
PATRIC	23741070. VBIStrCoe124346_5686.
Phylogenomic databases
HOGENOM	HBG285805.
OMA	RYLYDRE.
PhylomeDB	P0A4P1.
ProtClustDB	PRK00026.
Family and domain databases
HAMAP	MF_00605. TrmD. [Tree]
InterPro	IPR016009. tRNA_m1G_MeTrfase. IPR002649. tRNA_m1G_MeTrfase_bac. IPR023148. tRNA_m1G_MeTrfase_C. [Graphical view]
Gene3D	G3DSA:1.10.1270.20. tRNA_m1G_MeTrfase_C. 1 hit.
KO	K00554.
PANTHER	PTHR10056. PTHR10056. 1 hit.
Pfam	PF01746. tRNA_m1G_MT. 1 hit. [Graphical view]
PIRSF	PIRSF000386. tRNA_mtase. 1 hit.
TIGRFAMs	TIGR00088. TrmD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMD_STRCO

Accession

Primary (citable) accession number: P0A4P1
Secondary accession number(s): O69882

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	January 25, 2012
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program