Reviewed, UniProtKB/Swiss-Prot P0A4L6 (DSBA_ECO27)

Names and origin

Protein names

Recommended name:
    Thiol:disulfide interchange protein dsbA

Gene names

Name:	dsbA
Ordered Locus Names:	E2348_C_4167

Organism

Escherichia coli O127:H6 (strain E2348/69 / EPEC) [Complete proteome] [HAMAP]

Taxonomic identifier

574521 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	208 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Required for disulfide bond formation in some periplasmic proteins such as phoA or ompA. Acts by transferring its disulfide bond to other proteins and is reduced in the process. DsbA is reoxidized by dsbB. It is required for pilus biogenesis By similarity.
Subcellular location	Periplasm By similarity.
Sequence similarities	Belongs to the thioredoxin family. DsbA subfamily. Contains 1 thioredoxin domain.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Redox-active center Signal
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro
Cellular component	outer membrane-bounded periplasmic space Inferred from electronic annotation. Source: InterPro
Molecular function	protein disulfide oxidoreductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	By similarity
	Chain	20 – 208	189	Thiol:disulfide interchange protein dsbA		PRO_0000034253
Regions
	Domain	20 – 150	131	Thioredoxin
Amino acid modifications
	Disulfide bond	49 ↔ 52		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A4L6-1 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 01527E876ECAEE92	FASTA	208	23,119
	10 20 30 40 50 60 MKKIWLALAG LVLAFSASAA QYEDGKQYTT LEKPVAGAPQ VLEFFSFFCP HCYQFEEVLH 70 80 90 100 110 120 ISDNVKKKLP EGVKMTKYHV NFMGGDLGKE LTQAWAVAMA LGVEDKVTVP LFEGVQKTQT 130 140 150 160 170 180 IRSASDIRDV FINAGIKGEE YDAAWNSFVV KSLVAQQEKA AADVQLRGVP AMFVNGKYQL 190 200 NPQGMDTSNM DVFVQQYADT VKYLSEKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DsbA is required for stability of the type IV pilin of enteropathogenic Escherichia coli." Zhang H.Z., Donnenberg M.S. Mol. Microbiol. 21:787-797(1996) [PubMed: 8878041] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete genome sequence and comparative genome analysis of enteropathogenic Escherichia coli O127:H6 strain E2348/69." Iguchi A., Thomson N.R., Ogura Y., Saunders D., Ooka T., Henderson I.R., Harris D., Asadulghani M., Kurokawa K., Dean P., Kenny B., Quail M.A., Thurston S., Dougan G., Hayashi T., Parkhill J., Frankel G. J. Bacteriol. 191:347-354(2009) [PubMed: 18952797] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z54203 Genomic DNA. Translation: CAA90910.1. FM180568 Genomic DNA. Translation: CAS11715.1.
RefSeq	YP_002331625.1.
3D structure databases
HSSP	HSSP built from PDB template 1A23 based on UniProtKB P24991.
SMR	P0A4L6. Positions 20-207.
ModBase	Search...
Genome annotation databases
GeneID	7063974.
GenomeReviews	Gene locus E2348_C_4167 in contig FM180568_GR.
KEGG	ecg:E2348C_4167.
Organism-specific databases
CMR	Search...
Family and domain databases
InterPro	IPR001853. OxRdtase_DSBA. IPR017936. Thioredoxin-like. IPR017937. Thioredoxin_CS. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
Pfam	PF01323. DSBA. 1 hit. [Graphical view]
PROSITE	PS00194. THIOREDOXIN_1. 1 hit. PS51352. THIOREDOXIN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DSBA_ECO27

Accession

Primary (citable) accession number: P0A4L6
Secondary accession number(s): B7UNI3, P59589

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	July 7, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)