Reviewed, UniProtKB/Swiss-Prot P0A4L5 (DSBA_ECOL6)

Names and origin

Protein names

Recommended name:
    Thiol:disulfide interchange protein dsbA

Gene names

Name:	dsbA
Ordered Locus Names:	c4804

Organism

Escherichia coli O6 [Complete proteome] [HAMAP]

Taxonomic identifier

217992 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	208 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Required for disulfide bond formation in some periplasmic proteins such as phoA or ompA. Acts by transferring its disulfide bond to other proteins and is reduced in the process. DsbA is reoxidized by dsbB. It is required for pilus biogenesis By similarity.
Subcellular location	Periplasm By similarity.
Sequence similarities	Belongs to the thioredoxin family. DsbA subfamily. Contains 1 thioredoxin domain.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Redox-active center Signal
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro
Cellular component	outer membrane-bounded periplasmic space Inferred from electronic annotation. Source: InterPro
Molecular function	protein disulfide oxidoreductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	By similarity
	Chain	20 – 208	189	Thiol:disulfide interchange protein dsbA		PRO_0000034254
Regions
	Domain	20 – 150	131	Thioredoxin
Amino acid modifications
	Disulfide bond	49 ↔ 52		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A4L5-1 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 01527E876ECAEE92	FASTA	208	23,119
	10 20 30 40 50 60 MKKIWLALAG LVLAFSASAA QYEDGKQYTT LEKPVAGAPQ VLEFFSFFCP HCYQFEEVLH 70 80 90 100 110 120 ISDNVKKKLP EGVKMTKYHV NFMGGDLGKE LTQAWAVAMA LGVEDKVTVP LFEGVQKTQT 130 140 150 160 170 180 IRSASDIRDV FINAGIKGEE YDAAWNSFVV KSLVAQQEKA AADVQLRGVP AMFVNGKYQL 190 200 NPQGMDTSNM DVFVQQYADT VKYLSEKK

References

[1]

"Extensive mosaic structure revealed by the complete genome sequence of uropathogenic Escherichia coli." Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P., Rasko D., Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D., Mayhew G.F., Rose D.J., Zhou S., Schwartz D.C., Perna N.T., Mobley H.L.T., Donnenberg M.S., Blattner F.R. Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002) [PubMed: 12471157] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O6:H1 / CFT073 / ATCC 700928 / UPEC.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014075 Genomic DNA. Translation: AAN83235.1.
RefSeq	NP_756661.1.
3D structure databases
HSSP	HSSP built from PDB template 1A23 based on UniProtKB P24991.
SMR	P0A4L5. Positions 20-207.
ModBase	Search...
Genome annotation databases
GeneID	1036525.
GenomeReviews	Gene locus c4804 in contig AE014075_GR.
KEGG	ecc:c4804.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P0A4L5.
OMA	P0A4L5. EGVHYEV.
Family and domain databases
InterPro	IPR001853. OxRdtase_DSBA. IPR017936. Thioredoxin-like. IPR017937. Thioredoxin_CS. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
Pfam	PF01323. DSBA. 1 hit. [Graphical view]
PROSITE	PS00194. THIOREDOXIN_1. 1 hit. PS51352. THIOREDOXIN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DSBA_ECOL6

Accession

Primary (citable) accession number: P0A4L5
Secondary accession number(s): P59589

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	June 16, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)