P0A4K2 (METC_LACLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cystathionine beta-lyase
Short name=CBL
EC=4.4.1.8

Alternative name(s):
Beta-cystathionase
Cysteine lyase

Gene names

Name:	metC
Synonyms:	metB2
Ordered Locus Names:	LL0781
ORF Names:	L0181

Organism

Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)

Taxonomic identifier

272623 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Lactococcus

Protein attributes

Sequence length	380 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The enzymatic degradation of amino acids in cheese is believed to generate aroma compounds and therefore to be essential for flavor development. Cystathionine beta-lyase (CBL) can convert cystathionine to homocysteine but is also able to catalyze an alpha, gamma elimination. With methionine as a substrate, it produces volatile sulfur compounds which are important for flavor formation in Gouda cheese.
Catalytic activity	L-cystathionine + H2O = L-homocysteine + NH3 + pyruvate.
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homocysteine from L-cystathionine: step 1/1.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the trans-sulfuration enzymes family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cystathionine beta-lyase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 380	380	Cystathionine beta-lyase		PRO_0000114772
Amino acid modifications
	Modified residue	196	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A4K2 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 77A580507FE50B0D	FASTA	380	40,938
	10 20 30 40 50 60 MTSIKTKVIH GGISTDKTTG AVSVPIYQTS TYKQNGLGQP KEYEYSRSGN PTRHALEELI 70 80 90 100 110 120 ADLEGGVQGF AFSSGLAGIH AVLSLFSAGD HIILADDVYG GTFRLMDKVL TKTGIIYDLV 130 140 150 160 170 180 DLSNLDDLKA AFKEETKAIY FETPSNPLLK VLDIKEISAI AKAHDALTLV DNTFATPYLQ 190 200 210 220 230 240 QPIALGADIV LHSATKYLGG HSDVVAGLVT TNSKELASEI GFLQNSIGAV LGPQDSWLVQ 250 260 270 280 290 300 RGIKTLALRM EAHSANAQKI AEFLETSKAV SKVYYPGLNS HPGHEIAKKQ MSAFGGMISF 310 320 330 340 350 360 ELTDENAVKD FVENLSYFTL AESLGGVESL IEVPAVMTHA SIPKELREEI GIKDGLIRLS 370 380 VGVEAIEDLL TDIKEALEKK

References

[1]	"The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403." Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A. Genome Res. 11:731-753(2001) [PubMed: 11337471] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IL1403.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005176 Genomic DNA. Translation: AAK04879.1.
PIR	E86722.
RefSeq	NP_266937.1. NC_002662.1.
3D structure databases
ProteinModelPortal	P0A4K2.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1114408.
GenomeReviews	Gene locus LL0781 in contig AE005176_GR.
KEGG	lla:L0181.
NMPDR	fig|272623.1.peg.801.
PATRIC	22293858. VBILacLac136773_0836.
Phylogenomic databases
HOGENOM	HBG754729.
OMA	NQYEYSR.
ProtClustDB	PRK06176.
Enzyme and pathway databases
BioCyc	LLAC272623:L0181-MONOMER.
Family and domain databases
InterPro	IPR000277. Cys/Met-Metab_PyrdxlP-dep_enz. IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit. G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.
KO	K01760.
Pfam	PF01053. Cys_Met_Meta_PP. 1 hit. [Graphical view]
PIRSF	PIRSF001434. CGS. 1 hit.
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
PROSITE	PS00868. CYS_MET_METAB_PP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

METC_LACLA

Accession

Primary (citable) accession number: P0A4K2
Secondary accession number(s): Q9RAS7, Q9RAS9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program