P0A4I6 (CIAH_STRR6) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sensor protein CiaH
EC=2.7.13.3

Gene names

Name:	ciaH
Ordered Locus Names:	spr0708

Organism

Streptococcus pneumoniae (strain ATCC BAA-255 / R6) [Reference proteome] [HAMAP]

Taxonomic identifier

171101 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Streptococcus › Streptococcus pneumoniae

Protein attributes

Sequence length	444 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Member of the two-component regulatory system CiaH/CiaR. Involved in early steps of competence regulation and in penicillin susceptibility. Probably phosphorylates CiaR.
Catalytic activity	ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Contains 1 histidine kinase domain.

Ontologies

Keywords
Biological process	Competence Two-component regulatory system
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	establishment of competence for transformation Inferred from electronic annotation. Source: UniProtKB-KW signal transduction by phosphorylation Inferred from electronic annotation. Source: GOC
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphorelay sensor kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 444	444	Sensor protein CiaH		PRO_0000074723
Regions
	Transmembrane	21 – 41	21	Helical; Potential
	Transmembrane	183 – 203	21	Helical; Potential
	Domain	223 – 438	216	Histidine kinase
Amino acid modifications
	Modified residue	226	1	Phosphohistidine; by autocatalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A4I6 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 1D36CAE8D21D3337	FASTA	444	50,673
	10 20 30 40 50 60 MFSKLKKTWY ADDFSYFIRN FGVFTLIFST MTLIILQVMH SSLYTSVDDK LHGLSENPQA 70 80 90 100 110 120 VIQLAINRAT EEIKDLENAR ADASKVEIKP NVSSNTEVIL FDKDFTQLLS GNRFLGLDKI 130 140 150 160 170 180 KLEKKELGHI YQIQVFNSYG QEEIYRVILM ETNISSVSTN IKYAAVLINT SQLEQASQKH 190 200 210 220 230 240 EQLIVVVMAS FWILSLLASL YLARVSVRPL LESMQKQQSF VENASHELRT PLAVLQNRLE 250 260 270 280 290 300 TLFRKPEATI MDVSESIASS LEEVRNMRFL TTSLLNLARR DDGIKPELAE VPTSFFNTTF 310 320 330 340 350 360 TNYEMIASEN NRVFRFENRI HRTIVTDQLL LKQLMTILFD NAVKYTEEDG EIDFLISATD 370 380 390 400 410 420 RNLYLLVSDN GIGISTEDKK KIFDRFYRVD KARTRQKGGF GLGLSLAKQI VDALKGTVTV 430 440 KDNKPKGTIF EVKIAIQTPS KKKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A two-component signal-transducing system is involved in competence and penicillin susceptibility in laboratory mutants of Streptococcus pneumoniae." Guenzi E., Gasc A.M., Sicard M.A., Hakenbeck R. Mol. Microbiol. 12:505-515(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Genome of the bacterium Streptococcus pneumoniae strain R6." Hoskins J., Alborn W.E. Jr., Arnold J., Blaszczak L.C., Burgett S., DeHoff B.S., Estrem S.T., Fritz L., Fu D.-J., Fuller W., Geringer C., Gilmour R., Glass J.S., Khoja H., Kraft A.R., Lagace R.E., LeBlanc D.J., Lee L.N. , Lefkowitz E.J., Lu J., Matsushima P., McAhren S.M., McHenney M., McLeaster K., Mundy C.W., Nicas T.I., Norris F.H., O'Gara M., Peery R.B., Robertson G.T., Rockey P., Sun P.-M., Winkler M.E., Yang Y., Young-Bellido M., Zhao G., Zook C.A., Baltz R.H., Jaskunas S.R., Rosteck P.R. Jr., Skatrud P.L., Glass J.I. J. Bacteriol. 183:5709-5717(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-255 / R6.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X77249 Genomic DNA. Translation: CAA54466.1. AE007317 Genomic DNA. Translation: AAK99512.1.
PIR	D97960. S49545.
RefSeq	NP_358302.1. NC_003098.1.
3D structure databases
ProteinModelPortal	P0A4I6.
ModBase	Search...
Protein-protein interaction databases
STRING	171101.spr0708.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAK99512; AAK99512; spr0708.
GeneID	934244.
KEGG	spr:spr0708.
PATRIC	19701324. VBIStrPne107296_0784.
Phylogenomic databases
eggNOG	COG0642.
HOGENOM	HOG000223177.
KO	K14982.
OMA	MIVFWLI.
ProtClustDB	CLSK876906.
Family and domain databases
Gene3D	1.10.287.130. 1 hit. 3.30.565.10. 1 hit.
InterPro	IPR003594. HATPase_ATP-bd. IPR004358. Sig_transdc_His_kin-like_C. IPR003661. Sig_transdc_His_kin_sub1_dim/P. IPR005467. Sig_transdc_His_kinase_core. IPR009082. Sig_transdc_His_kinase_dimeric. [Graphical view]
Pfam	PF02518. HATPase_c. 1 hit. PF00512. HisKA. 1 hit. [Graphical view]
PRINTS	PR00344. BCTRLSENSOR.
SMART	SM00387. HATPase_c. 1 hit. SM00388. HisKA. 1 hit. [Graphical view]
SUPFAM	SSF55874. ATP_bd_ATPase. 1 hit. SSF47384. His_kin_homodim. 1 hit.
PROSITE	PS50109. HIS_KIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CIAH_STRR6

Accession

Primary (citable) accession number: P0A4I6
Secondary accession number(s): Q54955

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	May 1, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program