P0A4I5 (CIAH_STRPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sensor protein CiaH
EC=2.7.13.3

Gene names

Name:	ciaH
Ordered Locus Names:	SP_0799

Organism

Streptococcus pneumoniae [Complete proteome] [HAMAP]

Taxonomic identifier

1313 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	444 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Member of the two-component regulatory system CiaH/CiaR. Involved in early steps of competence regulation and in penicillin susceptibility. Probably phosphorylates CiaR By similarity.
Catalytic activity	ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Contains 1 histidine kinase domain.

Ontologies

Keywords
Biological process	Competence Two-component regulatory system
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	establishment of competence for transformation Inferred from electronic annotation. Source: UniProtKB-KW peptidyl-histidine phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW two-component sensor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 444	444	Sensor protein CiaH		PRO_0000074722
Regions
	Transmembrane	21 – 41	21	Helical; Potential
	Transmembrane	183 – 203	21	Helical; Potential
	Domain	223 – 438	216	Histidine kinase
Amino acid modifications
	Modified residue	226	1	Phosphohistidine; by autocatalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A4I5 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 1D36CAE8D21D3337	FASTA	444	50,673
	10 20 30 40 50 60 MFSKLKKTWY ADDFSYFIRN FGVFTLIFST MTLIILQVMH SSLYTSVDDK LHGLSENPQA 70 80 90 100 110 120 VIQLAINRAT EEIKDLENAR ADASKVEIKP NVSSNTEVIL FDKDFTQLLS GNRFLGLDKI 130 140 150 160 170 180 KLEKKELGHI YQIQVFNSYG QEEIYRVILM ETNISSVSTN IKYAAVLINT SQLEQASQKH 190 200 210 220 230 240 EQLIVVVMAS FWILSLLASL YLARVSVRPL LESMQKQQSF VENASHELRT PLAVLQNRLE 250 260 270 280 290 300 TLFRKPEATI MDVSESIASS LEEVRNMRFL TTSLLNLARR DDGIKPELAE VPTSFFNTTF 310 320 330 340 350 360 TNYEMIASEN NRVFRFENRI HRTIVTDQLL LKQLMTILFD NAVKYTEEDG EIDFLISATD 370 380 390 400 410 420 RNLYLLVSDN GIGISTEDKK KIFDRFYRVD KARTRQKGGF GLGLSLAKQI VDALKGTVTV 430 440 KDNKPKGTIF EVKIAIQTPS KKKK

References

[1]

"Complete genome sequence of a virulent isolate of Streptococcus pneumoniae." Tettelin H., Nelson K.E., Paulsen I.T., Eisen J.A., Read T.D., Peterson S.N., Heidelberg J.F., DeBoy R.T., Haft D.H., Dodson R.J., Durkin A.S., Gwinn M.L., Kolonay J.F., Nelson W.C., Peterson J.D., Umayam L.A., White O., Salzberg S.L. , Lewis M.R., Radune D., Holtzapple E.K., Khouri H.M., Wolf A.M., Utterback T.R., Hansen C.L., McDonald L.A., Feldblyum T.V., Angiuoli S.V., Dickinson T., Hickey E.K., Holt I.E., Loftus B.J., Yang F., Smith H.O., Venter J.C., Dougherty B.A., Morrison D.A., Hollingshead S.K., Fraser C.M. Science 293:498-506(2001) [PubMed: 11463916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-334 / TIGR4.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005672 Genomic DNA. Translation: AAK74936.1.
PIR	G95092.
RefSeq	NP_345296.1. NC_003028.3.
3D structure databases
ProteinModelPortal	P0A4I5.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTRT00000026371; EBSTRP00000025463; EBSTRG00000026371.
GeneID	930749.
GenomeReviews	Gene locus SP_0799 in contig AE005672_GR.
KEGG	spn:SP_0799.
PATRIC	19705969. VBIStrPne105772_0831.
TIGR	SP_0799.
Phylogenomic databases
GeneTree	EBGT00050000026967.
HOGENOM	HBG532068.
OMA	MIVFWLI.
ProtClustDB	CLSK876906.
Enzyme and pathway databases
BioCyc	SPNE170187-1:SP_0799-MONOMER.
Family and domain databases
InterPro	IPR003594. ATPase-like_ATP-bd. IPR004358. Sig_transdc_His_kin-like_C. IPR003661. Sig_transdc_His_kin_sub1_dim/P. IPR005467. Sig_transdc_His_kinase_core. IPR009082. Sig_transdc_His_kinase_dimeric. [Graphical view]
Gene3D	G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit.
KO	K14982.
Pfam	PF02518. HATPase_c. 1 hit. PF00512. HisKA. 1 hit. [Graphical view]
PRINTS	PR00344. BCTRLSENSOR.
SMART	SM00387. HATPase_c. 1 hit. SM00388. HisKA. 1 hit. [Graphical view]
SUPFAM	SSF55874. ATP_bd_ATPase. 1 hit. SSF47384. His_kin_homodim. 1 hit.
PROSITE	PS50109. HIS_KIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CIAH_STRPN

Accession

Primary (citable) accession number: P0A4I5
Secondary accession number(s): Q54955

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	January 25, 2012
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program