P0A4G4 (MTSA_STRP1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Metal ABC transporter substrate-binding lipoprotein

Gene names

Name:	mtsA
Ordered Locus Names:	SPy_0453, M5005_Spy0368

Organism

Streptococcus pyogenes serotype M1 [Complete proteome] [HAMAP]

Taxonomic identifier

301447 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Streptococcus › Streptococcus pyogenes

Protein attributes

Sequence length	310 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Part of an ATP-driven transport system for a metal; this protein has affinity for Zn2+, Fe3+ and Cu2+.
Subcellular location	Cell membrane; Lipid-anchor.
Sequence similarities	Belongs to the bacterial solute-binding protein 9 family. Lipoprotein receptor antigen (Lrai) subfamily.
Sequence caution	The sequence AAZ50986.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Copper transport Ion transport Iron transport Transport Zinc transport
Cellular component	Cell membrane Membrane
Domain	Signal
Ligand	Copper Iron Metal-binding Zinc
PTM	Lipoprotein Palmitate
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological_process	cell adhesion Inferred from electronic annotation. Source: InterPro copper ion transport Inferred from electronic annotation. Source: UniProtKB-KW iron ion homeostasis Inferred from electronic annotation. Source: UniProtKB-KW zinc ion transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 20

20

Probable

Chain

21 – 310

290

Metal ABC transporter substrate-binding lipoprotein

PRO_0000031894

Sites

Metal binding

68

1

Zinc By similarity

Metal binding

140

1

Zinc By similarity

Metal binding

206

1

Zinc By similarity

Metal binding

281

1

Zinc By similarity

Amino acid modifications

Lipidation

21

1

N-palmitoyl cysteine Probable

Lipidation

21

1

S-diacylglycerol cysteine Probable

Natural variations

Natural variant

77

1

V → A in strain: AP1.

Experimental info

Sequence conflict

26

1

T → A in AAD56936. Ref.1

Sequence conflict

26

1

T → A in AAD56939. Ref.1

Sequence conflict

30

1

K → E in AAD56936. Ref.1

Sequence conflict

44

1

A → G in AAD56936. Ref.1

Sequence conflict

49 – 50

2

AI → VM in AAD56936. Ref.1

Secondary structure

1

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310

Helix Strand Turn

Details...

Beta strand

33 – 40

8

Helix

41 – 51

11

Helix

52 – 54

3

Beta strand

55 – 59

5

Beta strand

67 – 69

3

Helix

74 – 82

9

Beta strand

84 – 88

5

Helix

100 – 107

8

Turn

112 – 114

3

Beta strand

115 – 118

4

Turn

119 – 122

4

Helix

141 – 143

3

Helix

145 – 162

18

Helix

164 – 166

3

Helix

167 – 190

24

Turn

191 – 194

4

Helix

197 – 199

3

Beta strand

202 – 207

6

Helix

210 – 216

7

Beta strand

220 – 226

7

Helix

234 – 246

13

Beta strand

252 – 255

4

Helix

261 – 270

10

Beta strand

274 – 278

5

Beta strand

280 – 282

3

Helix

293 – 308

16

Sequences

	Sequence		Length	Mass (Da)
	P0A4G4 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: B0F829EF1C72CADC	FASTA	310	34,358
	10 20 30 40 50 60 MGKRMSLILG AFLSVFLLVA CSSTGTKTAK SDKLKVVATN SIIADMTKAI AGDKIDLHSI 70 80 90 100 110 120 VPIGQDPHEY EPLPEDVEKT SNADVIFYNG INLEDGGQAW FTKLVKNAQK TKNKDYFAVS 130 140 150 160 170 180 DGIDVIYLEG ASEKGKEDPH AWLNLENGII YSKNIAKQLI AKDPKNKETY EKNLKAYVAK 190 200 210 220 230 240 LEKLDKEAKS KFDAIAENKK LIVTSEGCFK YFSKAYGVPS AYIWEINTEE EGTPDQISSL 250 260 270 280 290 300 IEKLKVIKPS ALFVESSVDR RPMETVSKDS GIPIYSEIFT DSIAKKGKPG DSYYAMMKWN 310 LDKISEGLAK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of a Streptococcus pyogenes ABC transporter with multiple specificity for metal cations." Janulczyk R., Pallon J., Bjoerck L. Mol. Microbiol. 34:596-606(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 30-39, CHARACTERIZATION. Strain: AP1 / Serotype M1 and ATCC 700294 / SF370 / Serotype M1.
[2]	"Complete genome sequence of an M1 strain of Streptococcus pyogenes." Ferretti J.J., McShan W.M., Ajdic D.J., Savic D.J., Savic G., Lyon K., Primeaux C., Sezate S., Suvorov A.N., Kenton S., Lai H.S., Lin S.P., Qian Y., Jia H.G., Najar F.Z., Ren Q., Zhu H., Song L. , White J., Yuan X., Clifton S.W., Roe B.A., McLaughlin R.E. Proc. Natl. Acad. Sci. U.S.A. 98:4658-4663(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700294 / SF370 / Serotype M1.
[3]	"Evolutionary origin and emergence of a highly successful clone of serotype M1 group A Streptococcus involved multiple horizontal gene transfer events." Sumby P., Porcella S.F., Madrigal A.G., Barbian K.D., Virtaneva K., Ricklefs S.M., Sturdevant D.E., Graham M.R., Vuopio-Varkila J., Hoe N.P., Musser J.M. J. Infect. Dis. 192:771-782(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-947 / MGAS5005 / Serotype M1.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF180520 Genomic DNA. Translation: AAD56936.1.
AF180521 Genomic DNA. Translation: AAD56939.1.
AE004092 Genomic DNA. Translation: AAK33468.1.
CP000017 Genomic DNA. Translation: AAZ50986.1. Different initiation.

RefSeq

NP_268747.1. NC_002737.1.
YP_281731.1. NC_007297.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3HH8	X-ray	1.87	A	22-310	[»]

ProteinModelPortal

P0A4G4.

SMR

P0A4G4. Positions 28-310.

ModBase

Search...

Protein-protein interaction databases

STRING

160490.SPy_0453.

Protein family/group databases

TCDB

3.A.1.15.6. ATP-binding cassette (ABC) superfamily.

Proteomic databases

PaxDb

P0A4G4.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAK33468; AAK33468; SPy_0453.
AAZ50986; AAZ50986; M5005_Spy0368.

GeneID

3572540.
900696.

KEGG

spy:SPy_0453.
spz:M5005_Spy_0368.

PATRIC

19714627. VBIStrPyo79812_0382.

Organism-specific databases

CMR

Search...

Phylogenomic databases

eggNOG

COG0803.

HOGENOM

HOG000180309.

KO

K11704.

OMA

NKETYEN.

ProtClustDB

CLSK876868.

Enzyme and pathway databases

BioCyc

SPYO160490:GJ81-381-MONOMER.
SPYO293653:GHFC-423-MONOMER.

Family and domain databases

InterPro

IPR006129. AdhesinB.
IPR006128. Lipoprotein_4.
IPR006127. Perip_solute-bd_prot_fam.
[Graphical view]

Pfam

PF01297. SBP_bac_9. 1 hit.
[Graphical view]

PRINTS

PR00691. ADHESINB.
PR00690. ADHESNFAMILY.

PROSITE

PS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P0A4G4.

Entry information

Entry name

MTSA_STRP1

Accession

Primary (citable) accession number: P0A4G4
Secondary accession number(s): Q490I2 , Q9A157, Q9RNI7, Q9RNJ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	May 29, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program