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Protein

Manganese ABC transporter substrate-binding lipoprotein

Gene

psaA

Organism
Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Part of an ATP-driven transport system for manganese. Also act as an adhesin which is involved on adherence to extracellular matrix. It is an important factor in pathogenesis and infection.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi67 – 671ZincCurated
Metal bindingi139 – 1391ZincCurated
Metal bindingi205 – 2051ZincCurated
Metal bindingi280 – 2801ZincCurated

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transport

Keywords - Ligandi

Manganese, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciSPNE170187:GHGN-1657-MONOMER.

Protein family/group databases

TCDBi3.A.1.15.14. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Manganese ABC transporter substrate-binding lipoprotein
Alternative name(s):
Pneumococcal surface adhesin A
Gene namesi
Name:psaA
Ordered Locus Names:SP_1650
OrganismiStreptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Taxonomic identifieri170187 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
Proteomesi
  • UP000000585 Componenti: Chromosome

Subcellular locationi

  • Cell membrane PROSITE-ProRule annotation; Lipid-anchor PROSITE-ProRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919CuratedAdd
BLAST
Chaini20 – 309290Manganese ABC transporter substrate-binding lipoproteinPRO_0000031891Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi20 – 201N-palmitoyl cysteineCurated
Lipidationi20 – 201S-diacylglycerol cysteineCurated

Keywords - PTMi

Lipoprotein, Palmitate

Interactioni

Protein-protein interaction databases

STRINGi170187.SpneT_02000954.

Structurei

Secondary structure

1
309
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi27 – 293Combined sources
Beta strandi33 – 397Combined sources
Helixi40 – 5011Combined sources
Helixi51 – 533Combined sources
Beta strandi54 – 607Combined sources
Beta strandi66 – 683Combined sources
Helixi73 – 819Combined sources
Beta strandi83 – 875Combined sources
Turni90 – 945Combined sources
Turni96 – 983Combined sources
Helixi99 – 1068Combined sources
Turni111 – 1133Combined sources
Beta strandi114 – 1174Combined sources
Turni118 – 1214Combined sources
Helixi127 – 1293Combined sources
Helixi140 – 1423Combined sources
Helixi144 – 16118Combined sources
Helixi163 – 1653Combined sources
Helixi166 – 19328Combined sources
Helixi196 – 1983Combined sources
Beta strandi201 – 2066Combined sources
Helixi209 – 2157Combined sources
Beta strandi219 – 2235Combined sources
Helixi233 – 24412Combined sources
Beta strandi251 – 2544Combined sources
Helixi260 – 26910Combined sources
Beta strandi273 – 2775Combined sources
Beta strandi279 – 2813Combined sources
Helixi292 – 30716Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1PSZX-ray2.00A19-309[»]
3ZK7X-ray1.69A/B32-309[»]
3ZK8X-ray1.65A/B32-309[»]
3ZK9X-ray1.45A/B32-309[»]
3ZKAX-ray1.55A/B32-309[»]
3ZTTX-ray2.70A/B/C/D19-309[»]
4UTOX-ray1.55A/B1-309[»]
4UTPX-ray2.00A/B1-309[»]
ProteinModelPortaliP0A4G2.
SMRiP0A4G2. Positions 24-309.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A4G2.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105DSE. Bacteria.
COG0803. LUCA.
KOiK19971.
OMAiHAWQNVA.
OrthoDBiEOG6B3601.

Family and domain databases

InterProiIPR006129. AdhesinB.
IPR006128. Lipoprotein_4.
IPR006127. ZnuA-like.
[Graphical view]
PfamiPF01297. ZnuA. 1 hit.
[Graphical view]
PRINTSiPR00691. ADHESINB.
PR00690. ADHESNFAMILY.
PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A4G2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKLGTLLVL FLSAIILVAC ASGKKDTTSG QKLKVVATNS IIADITKNIA
60 70 80 90 100
GDKIDLHSIV PIGQDPHEYE PLPEDVKKTS EADLIFYNGI NLETGGNAWF
110 120 130 140 150
TKLVENAKKT ENKDYFAVSD GVDVIYLEGQ NEKGKEDPHA WLNLENGIIF
160 170 180 190 200
AKNIAKQLSA KDPNNKEFYE KNLKEYTDKL DKLDKESKDK FNKIPAEKKL
210 220 230 240 250
IVTSEGAFKY FSKAYGVPSA YIWEINTEEE GTPEQIKTLV EKLRQTKVPS
260 270 280 290 300
LFVESSVDDR PMKTVSQDTN IPIYAQIFTD SIAEQGKEGD SYYSMMKYNL

DKIAEGLAK
Length:309
Mass (Da):34,594
Last modified:March 15, 2005 - v1
Checksum:iB125E7FE3DA6F67C
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti8 – 81L → F in strain: NA-1508/92.
Natural varianti9 – 91V → I in strain: NA-1064/97.
Natural varianti14 – 141A → V in strain: NA-1064/97, NA-1383/97 and NA-1508/92.
Natural varianti16 – 161I → A in strain: NA-1064/97 and NA-1383/97.
Natural varianti16 – 161I → V in strain: NA-1508/92.
Natural varianti27 – 282TT → AA in strain: NA-1064/97, NA-1383/97 and NA-1508/92.
Natural varianti30 – 301G → S in strain: NA-1064/97.
Natural varianti62 – 621I → V in strain: NA-1383/97.
Natural varianti81 – 811E → Q in strain: NA-1383/97.
Natural varianti83 – 831D → N in strain: TIGR4.
Natural varianti120 – 1201D → E in strain: NA-1064/97, NA-1383/97 and NA-1508/92.
Natural varianti130 – 1301Q → K in strain: NA-1064/97 and NA-1508/92.
Natural varianti148 – 1481I → M in strain: NA-1383/97.
Natural varianti164 – 1641N → S in strain: NA-1383/97.
Natural varianti187 – 1893SKD → AKE in strain: NA-1383/97.
Natural varianti193 – 1931K → N in strain: NA-1064/97, NA-1383/97 and NA-1508/92.
Natural varianti207 – 2071A → C in strain: NA-1383/97.
Natural varianti234 – 2341E → D in strain: NA-1383/97.
Natural varianti248 – 2481V → T in strain: NA-1383/97.
Natural varianti285 – 2851Q → E in strain: NA-1508/92.
Natural varianti294 – 2941S → N in strain: NA-1383/97.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U53509 Genomic DNA. Translation: AAB09440.1.
U40786 Genomic DNA. Translation: AAC24470.1.
AF055088 Genomic DNA. Translation: AAD09975.1.
AF248229 Genomic DNA. Translation: AAF70663.1.
AF248231 Genomic DNA. Translation: AAF70665.1.
AF248232 Genomic DNA. Translation: AAF70666.1.
AF248233 Genomic DNA. Translation: AAF70667.1.
AF248234 Genomic DNA. Translation: AAF70668.1.
AE005672 Genomic DNA. Translation: AAK75729.1.
PIRiH95191.
RefSeqiWP_000733059.1. NZ_AKVY01000001.1.

Genome annotation databases

EnsemblBacteriaiAAK75729; AAK75729; SP_1650.
KEGGispn:SP_1650.
PATRICi19707735. VBIStrPne105772_1710.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U53509 Genomic DNA. Translation: AAB09440.1.
U40786 Genomic DNA. Translation: AAC24470.1.
AF055088 Genomic DNA. Translation: AAD09975.1.
AF248229 Genomic DNA. Translation: AAF70663.1.
AF248231 Genomic DNA. Translation: AAF70665.1.
AF248232 Genomic DNA. Translation: AAF70666.1.
AF248233 Genomic DNA. Translation: AAF70667.1.
AF248234 Genomic DNA. Translation: AAF70668.1.
AE005672 Genomic DNA. Translation: AAK75729.1.
PIRiH95191.
RefSeqiWP_000733059.1. NZ_AKVY01000001.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1PSZX-ray2.00A19-309[»]
3ZK7X-ray1.69A/B32-309[»]
3ZK8X-ray1.65A/B32-309[»]
3ZK9X-ray1.45A/B32-309[»]
3ZKAX-ray1.55A/B32-309[»]
3ZTTX-ray2.70A/B/C/D19-309[»]
4UTOX-ray1.55A/B1-309[»]
4UTPX-ray2.00A/B1-309[»]
ProteinModelPortaliP0A4G2.
SMRiP0A4G2. Positions 24-309.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi170187.SpneT_02000954.

Protein family/group databases

TCDBi3.A.1.15.14. the atp-binding cassette (abc) superfamily.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK75729; AAK75729; SP_1650.
KEGGispn:SP_1650.
PATRICi19707735. VBIStrPne105772_1710.

Phylogenomic databases

eggNOGiENOG4105DSE. Bacteria.
COG0803. LUCA.
KOiK19971.
OMAiHAWQNVA.
OrthoDBiEOG6B3601.

Enzyme and pathway databases

BioCyciSPNE170187:GHGN-1657-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0A4G2.

Family and domain databases

InterProiIPR006129. AdhesinB.
IPR006128. Lipoprotein_4.
IPR006127. ZnuA-like.
[Graphical view]
PfamiPF01297. ZnuA. 1 hit.
[Graphical view]
PRINTSiPR00691. ADHESINB.
PR00690. ADHESNFAMILY.
PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Streptococcus pneumoniae surface adhesin A."
    Sampson J.S., Whitney A.M., Furlow Z.
    Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 6B.
  2. "Sequence heterogeneity of PsaA, a 37-kilodalton putative adhesin essential for virulence of Streptococcus pneumoniae."
    Berry A.M., Paton J.C.
    Infect. Immun. 64:5255-5262(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: D39 / NCTC 7466 / Serotype 2.
  3. "Penicillin tolerance genes of Streptococcus pneumoniae: the ABC-type manganese permease complex Psa."
    Novak R., Braun J.S., Charpentier E., Tuomanen E.
    Mol. Microbiol. 29:1285-1296(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Identification of a psaA gene in viridans streptococcal strains."
    Perez A., Jado I., Casal J.
    Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: NA-1064/97, NA-1283/96, NA-1383/97 and NA-1508/92.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-334 / TIGR4.
  6. "Competence and virulence of Streptococcus pneumoniae: Adc and PsaA mutants exhibit a requirement for Zn and Mn resulting from inactivation of putative ABC metal permeases."
    Dintilhac A., Alloing G., Granadel C., Claverys J.-P.
    Mol. Microbiol. 25:727-739(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "The crystal structure of pneumococcal surface antigen PsaA reveals a metal-binding site and a novel structure for a putative ABC-type binding protein."
    Lawrence M.C., Pilling P.A., Epa V.C., Berry A.M., Ogunniyi A.D., Paton J.C.
    Structure 6:1553-1561(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 19-309, METAL-BINDING SITES.

Entry informationi

Entry nameiMTSA_STRPN
AccessioniPrimary (citable) accession number: P0A4G2
Secondary accession number(s): P72538
, Q54720, Q9L5X2, Q9L5X3, Q9L5X4, Q9R6P5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: May 11, 2016
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.