P0A4E0 (RNH2_BRUSU) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 40.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Ribonuclease HII Short name=RNase HII EC=3.1.26.4 | ||||
| Gene names |
| ||||
| Organism | Brucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP] | ||||
| Taxonomic identifier | 204722 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella |
Protein attributes
| Sequence length | 220 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology |
General annotation (Comments)
| Function | Endonuclease that specifically degrades the RNA of RNA-DNA hybrids By similarity. HAMAP MF_00052_B |
| Catalytic activity | Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP MF_00052_B |
| Cofactor | Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding By similarity. |
| Subcellular location | Cytoplasm Potential HAMAP MF_00052_B. |
| Sequence similarities | Belongs to the RNase HII family. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cytoplasm |
| Ligand | Manganese Metal-binding |
| Molecular function | Endonuclease Hydrolase Nuclease |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Cellular component | cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | RNA binding Inferred from electronic annotation. Source: InterPro metal ion bindingInferred from electronic annotation. Source: UniProtKB-KW ribonuclease H activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 220 | 220 | Ribonuclease HII HAMAP MF_00052_B | PRO_0000111552 | |||||
Sites | |||||||||
| Metal binding | 38 | 1 | Divalent metal cation By similarity | ||||||
| Metal binding | 39 | 1 | Divalent metal cation By similarity | ||||||
| Metal binding | 130 | 1 | Divalent metal cation By similarity | ||||||
Sequences
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References
| [1] | "The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. Fraser C.M.Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed: 12271122] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330. |
| [2] | "Revised genome sequence of Brucella suis 1330." Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R. J. Bacteriol. 193:6410-6410(2011) [PubMed: 22038969] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AE014291 Genomic DNA. Translation: AAN29332.1. CP002997 Genomic DNA. Translation: AEM17745.1. |
| RefSeq | NP_697417.1. NC_004310.3. |
3D structure databases | |
| ProteinModelPortal | P0A4E0. |
| ModBase | Search... |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| GeneID | 1166047. |
| GenomeReviews | Gene locus BR0386 in contig AE014291_GR. |
| KEGG | bms:BR0386. |
| PATRIC | 17789068. VBIBruSui107850_0402. |
| TIGR | BR0386. |
Phylogenomic databases | |
| HOGENOM | HBG584843. |
| OMA | RLGPTPI. |
| PhylomeDB | P0A4E0. |
| ProtClustDB | PRK00015. |
Enzyme and pathway databases | |
| BioCyc | BSUI204722:BR_0386-MONOMER. |
Family and domain databases | |
| HAMAP | MF_00052_B. RNase_HII_B. [Tree] |
| InterPro | IPR022898. RNase_HII. IPR001352. RNase_HII/HIII. IPR024567. RNase_HII/HIII_dom. IPR012337. RNaseH-like_dom. [Graphical view] |
| KO | K03470. |
| PANTHER | PTHR10954. RNase_HII/HIII. 1 hit. |
| Pfam | PF01351. RNase_HII. 1 hit. [Graphical view] |
| SUPFAM | SSF53098. RNaseH_fold. 1 hit. |
| ProtoNet | Search... |
Entry information
| Entry name | RNH2_BRUSU | ||||||||
| Accession | Primary (citable) accession number: P0A4E0 Secondary accession number(s): G0K6K8, O68821 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Prokaryotic Protein Annotation Program | ||||||||
Relevant documents
| Brucella suis Brucella suis (strain 1330): entries and gene names |
| SIMILARITY comments Index of protein domains and families |

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