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Reviewed, UniProtKB/Swiss-Prot P0A4E0 (RNH2_BRUSU)

Last modified November 24, 2009. Version 28. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Ribonuclease HII
      Short name=RNase HII
    EC=3.1.26.4
Gene names
Name: rnhB
Ordered Locus Names: BR0386
OrganismBrucella suis [Complete proteome] [HAMAP]
Taxonomic identifier29461 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella

Protein attributes

Sequence length220 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

General annotation (Comments)

Function

Endonuclease that specifically degrades the RNA of RNA-DNA hybrids By similarity.

Catalytic activity

Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP MF_00052

Cofactor

Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding By similarity.

Subcellular location

Cytoplasm Potential.

Sequence similarities

Belongs to the RNase HII family.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandManganese
Metal-binding
   Molecular functionEndonuclease
Hydrolase
Nuclease
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processRNA catabolic process

Inferred from electronic annotation. Source: HAMAP

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionRNA binding

Inferred from electronic annotation. Source: InterPro

manganese ion binding

Inferred from electronic annotation. Source: HAMAP

ribonuclease H activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 220220Ribonuclease HII HAMAP MF_00052
PRO_0000111552

Sites

Metal binding381Divalent metal cation By similarity
Metal binding391Divalent metal cation By similarity
Metal binding1301Divalent metal cation By similarity

Sequences

Sequence LengthMass (Da)Tools
P0A4E0-1 [UniParc].

Last modified March 15, 2005. Version 1.
Checksum: 87E3AB0664844E8E

FASTA22023,385
        10         20         30         40         50         60 
MKRSASDSPL LFDLPLAPDF SQEQQLMKRG LKHIAGIDEA GRGPLAGPVV AAAVVLDQND 

        70         80         90        100        110        120 
LPEGLDDSKR LTAARREALY EIILTKAITV SVASLSARSI DASDIRKAAL EAMRRAVIGL 

       130        140        150        160        170        180 
TLKPCHALVD GRDVPPGLPC PGSALVKGDQ RSVSIAAASI VAKVTRDRMM IRAGAAHPPY 

       190        200        210        220 
GLEIHAGYAT QKHRAAIESE GPVPGLHRYT FAPIKGRFDC 

« Hide

Cross-references

Sequence databases

AE014291 Genomic DNA. Translation: AAN29332.1.
RefSeqNP_697417.1.

3D structure databases

ModBaseSearch...

Genome annotation databases

GeneID1166047.
GenomeReviewsGene locus BR0386 in contig AE014291_GR.
KEGGbms:BR0386.
TIGRBR0386.

Phylogenomic databases

HOGENOMP0A4E0.
OMARLGPTPI

Enzyme and pathway databases

BioCycBSUI204722:BR_0386-MON.
BRENDA3.1.26.4. 281610.

Family and domain databases

HAMAPMF_00052.
[Tree]
InterProIPR012337. PolynucTfrase_RNaseH_fold.
IPR001352. RNase_HII/HIII.
[Graphical view]
PANTHERPTHR10954. RNase_HII/HIII. 1 hit.
PfamPF01351. RNase_HII. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRNH2_BRUSU
AccessionPrimary (citable) accession number: P0A4E0
Secondary accession number(s): O68821
Entry history
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: November 24, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Brucella suis

Brucella suis (strain 1330): entries and gene names

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents