Reviewed,
UniProtKB/Swiss-Prot P0A462 (MTA1_ANAVA)
Last modified
January 19, 2010.
Version 24.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Modification methylase AvaI Short name=M.AvaI EC=2.1.1.113 Alternative name(s): N(4)- cytosine-specific methyltransferase AvaI | ||
| Gene names |
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| Organism | Anabaena variabilis | ||
| Taxonomic identifier | 1172 [NCBI] | ||
| Taxonomic lineage | Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Anabaena |
Protein attributes
| Sequence length | 482 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology. |
General annotation (Comments)
| Function | This methylase recognizes the double-stranded sequence CYCGRG, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the avaI endonuclease. |
| Catalytic activity | S-adenosyl-L-methionine + DNA cytosine = S-adenosyl-L-homocysteine + DNA N(4)-methylcytosine. |
| Sequence similarities | Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Restriction system |
| Ligand | S-adenosyl-L-methionine |
| Molecular function | Methyltransferase Transferase |
| Gene Ontology (GO) | |
| Biological process | DNA methylation on cytosine Inferred from electronic annotation. Source: InterPro DNA restriction-modification systemInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | DNA binding Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (cytosine-N4-specific) activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||
Molecule processing | |||||||
|---|---|---|---|---|---|---|---|
| Chain | 1 – 482 | 482 | Modification methylase AvaI | PRO_0000087919 | |||
Sequences
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References
| [1] | "Cloning and sequence comparison of AvaI and BsoBI restriction-modification systems." Ruan H., Lunnen K.D., Scott M.E., Moran L.S., Slatko B.E., Pelletier J.J., Hess E.J., Benner J. II, Wilson G.G., Xu S.-Y. Mol. Gen. Genet. 252:695-699(1996) [PubMed: 8917312] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: PCC 7118 / ATCC 27892. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | X98339 Genomic DNA. Translation: CAA66984.1. |
| PIR | S72471. |
3D structure databases | |
| SMR | P0A462. Positions 281-461. |
| ModBase | Search... |
Enzyme and pathway databases | |
| BRENDA | 2.1.1.113. 2836. |
Family and domain databases | |
| InterPro | IPR017985. MeTrfase_CN4_CS. [Graphical view] |
| PROSITE | PS00093. N4_MTASE. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | MTA1_ANAVA | ||||||||
| Accession | Primary (citable) accession number: P0A462 Secondary accession number(s): P70802 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| Restriction enzymes and methylases Classification of restriction enzymes and methylases and list of entries |
| SIMILARITY comments Index of protein domains and families |

Clusters with


