P0A461 (MTA1_NOSS1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Modification methylase AvaI
Short name=M.AvaI
EC=2.1.1.113

Alternative name(s):
N(4) cytosine-specific methyltransferase AvaI

Gene names

Name:	avaIM
Ordered Locus Names:	all3632

Organism

Nostoc sp. (strain PCC 7120 / UTEX 2576) [Complete proteome] [HAMAP]

Taxonomic identifier

103690 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc

Protein attributes

Sequence length	482 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence CYCGRG, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the AvaI endonuclease By similarity.
Catalytic activity	S-adenosyl-L-methionine + DNA cytosine = S-adenosyl-L-homocysteine + DNA N(4)-methylcytosine.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (cytosine-N4-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 482	482	Modification methylase AvaI		PRO_0000087918

Sequences

	Sequence		Length	Mass (Da)
	P0A461 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 795ED0B445EA9E0C	FASTA	482	56,579
	10 20 30 40 50 60 MTSFELESPI EIKTDPTDLD QESDSFVQEI SRFNKALEQR FRDKMRLHES LSRKIVSFQA 70 80 90 100 110 120 NKSKPQYRWF KYKEAFSVDL VNQLIFEYEK KSFERILDPF AGAGTMLFAC SDAGIQADGI 130 140 150 160 170 180 EVLPIGQEII EVRKIIQRQF RREDFLRLIE WYKQKPWNQH NNRKYLNRLR ITDGAYPPET 190 200 210 220 230 240 EASIERFLFS IEKENILVKQ VLRFALLCIL ESISYTRKDG QYLRWDKRAF RKSGSDKFDK 250 260 270 280 290 300 GKILDFDEAI TEQIKLILND SFDLISNTLF CYGTQRSGIN LFNASCLKIL PEFEQDFYDC 310 320 330 340 350 360 IITSPPYCNR YDYTRTYALE LALLGVGERD IVQLRQDMLS CTVENKEKSL IHNWQEALRI 370 380 390 400 410 420 LDKQELLQSI LRFLERELER KKLNNNGIPR MIKGYFYEMA CVIIECFRVL KNGSPLFMVN 430 440 450 460 470 480 DNVRYAGIDI SVDLILSNIA EEIGFNVEKI LVLPTGKGNS SQQMGTHGRK TLRKCVYVWR KP

References

[1]

"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120." Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. , Takazawa M., Yamada M., Yasuda M., Tabata S. DNA Res. 8:205-213(2001) [PubMed: 11759840] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 7120 / UTEX 2576.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000019 Genomic DNA. Translation: BAB75331.1.
PIR	AI2259.
RefSeq	NP_487672.1. NC_003272.1.
3D structure databases
ProteinModelPortal	P0A461.
ModBase	Search...
Protein-protein interaction databases
STRING	P0A461.
Protein family/group databases
REBASE	3287. M.AvaI.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1107230.
GenomeReviews	Gene locus all3632 in contig BA000019_GR.
KEGG	ana:all3632.
NMPDR	fig|103690.1.peg.3939.
PATRIC	22777714. VBINosSp37423_4311.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0863.
HOGENOM	HBG441497.
OMA	NSSQQMG.
ProtClustDB	CLSK896011.
Enzyme and pathway databases
BioCyc	NSP103690:ALL3632-MONOMER.
Family and domain databases
InterPro	IPR017985. MeTrfase_CN4_CS. [Graphical view]
KO	K00590.
PROSITE	PS00093. N4_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTA1_NOSS1

Accession

Primary (citable) accession number: P0A461
Secondary accession number(s): P70802

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	January 25, 2012
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program