P0A460 (T2D1_STRR6) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type-2 restriction enzyme DpnI
Short name=R.DpnI
EC=3.1.21.4

Alternative name(s):
Endonuclease DpnI
Type II restriction enzyme DpnI

Gene names

Name:	dpnC
Ordered Locus Names:	spr1665

Organism

Streptococcus pneumoniae (strain ATCC BAA-255 / R6) [Reference proteome] [HAMAP]

Taxonomic identifier

171101 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Streptococcus › Streptococcus pneumoniae

Protein attributes

Sequence length	254 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Recognizes the double-stranded and methylated sequence G(Me)ATC and cleaves after A-2 By similarity.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Molecular_function	Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 254

254

Type-2 restriction enzyme DpnI

PRO_0000077301

Secondary structure

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254

Helix Strand Turn

Details...

Helix

7 – 12

6

Turn

13 – 15

3

Helix

19 – 31

13

Turn

35 – 37

3

Beta strand

53 – 55

3

Turn

57 – 59

3

Beta strand

62 – 71

10

Beta strand

74 – 80

7

Helix

81 – 89

9

Beta strand

95 – 101

7

Beta strand

105 – 113

9

Helix

115 – 117

3

Helix

120 – 122

3

Beta strand

123 – 129

7

Beta strand

138 – 144

7

Helix

145 – 147

3

Helix

150 – 152

3

Beta strand

154 – 158

5

Helix

165 – 174

10

Helix

175 – 180

6

Helix

183 – 198

16

Beta strand

201 – 205

5

Helix

206 – 210

5

Helix

213 – 219

7

Helix

226 – 239

14

Beta strand

242 – 245

4

Beta strand

250 – 253

4

Sequences

	Sequence		Length	Mass (Da)
	P0A460 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 7FFFFE14BB3EFEDB	FASTA	254	29,785
	10 20 30 40 50 60 MELHFNLELV ETYKSNSQKA RILTEDWVYR QSYCPNCGNN PLNHFENNRP VADFYCNHCS 70 80 90 100 110 120 EEFELKSKKG NFSSTINDGA YATMMKRVQA DNNPNFFFLT YTKNFEVNNF LVLPKQFVTP 130 140 150 160 170 180 KSIIQRKPLA PTARRAGWIG CNIDLSQVPS KGRIFLVQDG QVRDPEKVTK EFKQGLFLRK 190 200 210 220 230 240 SSLSSRGWTI EILNCIDKIE GSEFTLEDMY RFESDLKNIF VKNNHIKEKI RQQLQILRDK 250 EIIEFKGRGK YRKL

References

[1]

"Genome of the bacterium Streptococcus pneumoniae strain R6." Hoskins J., Alborn W.E. Jr., Arnold J., Blaszczak L.C., Burgett S., DeHoff B.S., Estrem S.T., Fritz L., Fu D.-J., Fuller W., Geringer C., Gilmour R., Glass J.S., Khoja H., Kraft A.R., Lagace R.E., LeBlanc D.J., Lee L.N. , Lefkowitz E.J., Lu J., Matsushima P., McAhren S.M., McHenney M., McLeaster K., Mundy C.W., Nicas T.I., Norris F.H., O'Gara M., Peery R.B., Robertson G.T., Rockey P., Sun P.-M., Winkler M.E., Yang Y., Young-Bellido M., Zhao G., Zook C.A., Baltz R.H., Jaskunas S.R., Rosteck P.R. Jr., Skatrud P.L., Glass J.I. J. Bacteriol. 183:5709-5717(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-255 / R6.

+

Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE007317 Genomic DNA. Translation: AAL00468.1.

PIR

G98079.

RefSeq

NP_359257.1. NC_003098.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
4ESJ	X-ray	2.05	A/B	1-254	[»]

ProteinModelPortal

P0A460.

ModBase

Search...

Protein-protein interaction databases

STRING

171101.spr1665.

Protein family/group databases

REBASE

5630. SpnRORF1665P.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAL00468; AAL00468; spr1665.

GeneID

933882.

KEGG

spr:spr1665.

PATRIC

19703368. VBIStrPne107296_1799.

Phylogenomic databases

eggNOG

NOG11693.

HOGENOM

HOG000067092.

KO

K01155.

OMA

HHIMCSI.

ProtClustDB

CLSK878276.

Family and domain databases

InterPro

IPR010324. DRP.
[Graphical view]

Pfam

PF06044. DRP. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

T2D1_STRR6

Accession

Primary (citable) accession number: P0A460
Secondary accession number(s): P09356

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	May 1, 2013
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program