P0A460 (T2D1_STRR6) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type-2 restriction enzyme DpnI
Short name=R.DpnI
EC=3.1.21.4

Alternative name(s):
Endonuclease DpnI
Type II restriction enzyme DpnI

Gene names

Name:	dpnC
Ordered Locus Names:	spr1665

Organism

Streptococcus pneumoniae (strain ATCC BAA-255 / R6)

Taxonomic identifier

171101 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	254 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Recognizes the double-stranded and methylated sequence G(Me)ATC and cleaves after A-2 By similarity.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 254	254	Type-2 restriction enzyme DpnI		PRO_0000077301

Sequences

	Sequence		Length	Mass (Da)
	P0A460 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 7FFFFE14BB3EFEDB	FASTA	254	29,785
	10 20 30 40 50 60 MELHFNLELV ETYKSNSQKA RILTEDWVYR QSYCPNCGNN PLNHFENNRP VADFYCNHCS 70 80 90 100 110 120 EEFELKSKKG NFSSTINDGA YATMMKRVQA DNNPNFFFLT YTKNFEVNNF LVLPKQFVTP 130 140 150 160 170 180 KSIIQRKPLA PTARRAGWIG CNIDLSQVPS KGRIFLVQDG QVRDPEKVTK EFKQGLFLRK 190 200 210 220 230 240 SSLSSRGWTI EILNCIDKIE GSEFTLEDMY RFESDLKNIF VKNNHIKEKI RQQLQILRDK 250 EIIEFKGRGK YRKL

References

[1]

"Genome of the bacterium Streptococcus pneumoniae strain R6." Hoskins J., Alborn W.E. Jr., Arnold J., Blaszczak L.C., Burgett S., DeHoff B.S., Estrem S.T., Fritz L., Fu D.-J., Fuller W., Geringer C., Gilmour R., Glass J.S., Khoja H., Kraft A.R., Lagace R.E., LeBlanc D.J., Lee L.N. , Lefkowitz E.J., Lu J., Matsushima P., McAhren S.M., McHenney M., McLeaster K., Mundy C.W., Nicas T.I., Norris F.H., O'Gara M., Peery R.B., Robertson G.T., Rockey P., Sun P.-M., Winkler M.E., Yang Y., Young-Bellido M., Zhao G., Zook C.A., Baltz R.H., Jaskunas S.R., Rosteck P.R. Jr., Skatrud P.L., Glass J.I. J. Bacteriol. 183:5709-5717(2001) [PubMed: 11544234] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-255 / R6.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE007317 Genomic DNA. Translation: AAL00468.1.
PIR	G98079.
RefSeq	NP_359257.1. NC_003098.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P0A460.
Protein family/group databases
REBASE	5630. SpnRORF1665P.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTRT00000015215; EBSTRP00000014611; EBSTRG00000015215.
GeneID	933882.
GenomeReviews	Gene locus spr1665 in contig AE007317_GR.
KEGG	spr:spr1665.
PATRIC	19703368. VBIStrPne107296_1799.
Phylogenomic databases
eggNOG	NOG11693.
GeneTree	EBGT00050000030012.
HOGENOM	HBG302213.
OMA	QYELKSK.
ProtClustDB	CLSK878276.
Enzyme and pathway databases
BioCyc	SPNE171101:SPR1665-MONOMER.
Family and domain databases
InterPro	IPR010324. DRP. [Graphical view]
KO	K01155.
Pfam	PF06044. DRP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T2D1_STRR6

Accession

Primary (citable) accession number: P0A460
Secondary accession number(s): P09356

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	January 25, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program