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Reviewed, UniProtKB/Swiss-Prot P0A459 (T2D1_STRPN)

Last modified November 3, 2009. Version 22. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Type-2 restriction enzyme DpnI
      Short name=R.DpnI
    EC=3.1.21.4
Alternative name(s):
    Type II restriction enzyme DpnI
    Endonuclease DpnI
Gene names
Name: dpnC
Ordered Locus Names: SP_1850
OrganismStreptococcus pneumoniae [Complete proteome] [HAMAP]
Taxonomic identifier1313 [NCBI]
Taxonomic lineageBacteriaFirmicutesLactobacillalesStreptococcaceaeStreptococcus

Protein attributes

Sequence length254 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existencePredicted.

General annotation (Comments)

Function

Recognizes the double-stranded and methylated sequence G(Me)ATC and cleaves after A-2.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
   Biological processRestriction system
   Molecular functionEndonuclease
Hydrolase
Nuclease
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionType II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 254254Type-2 restriction enzyme DpnI
PRO_0000077300

Sequences

Sequence LengthMass (Da)Tools
P0A459-1 [UniParc].

Last modified March 15, 2005. Version 1.
Checksum: 7FFFFE14BB3EFEDB

FASTA25429,785
        10         20         30         40         50         60 
MELHFNLELV ETYKSNSQKA RILTEDWVYR QSYCPNCGNN PLNHFENNRP VADFYCNHCS 

        70         80         90        100        110        120 
EEFELKSKKG NFSSTINDGA YATMMKRVQA DNNPNFFFLT YTKNFEVNNF LVLPKQFVTP 

       130        140        150        160        170        180 
KSIIQRKPLA PTARRAGWIG CNIDLSQVPS KGRIFLVQDG QVRDPEKVTK EFKQGLFLRK 

       190        200        210        220        230        240 
SSLSSRGWTI EILNCIDKIE GSEFTLEDMY RFESDLKNIF VKNNHIKEKI RQQLQILRDK 

       250 
EIIEFKGRGK YRKL 

« Hide

References

« Hide 'large scale' references
[1]"Proteins encoded by the DpnI restriction gene cassette. Hyperproduction and characterization of the DpnI endonuclease."
de la Campa A.G., Springhorn S.S., Kale P., Lacks S.A.
J. Biol. Chem. 263:14696-14702(1988) [PubMed: 2844782] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Genetic basis of the complementary DpnI and DpnII restriction systems of S. pneumoniae: an intercellular cassette mechanism."
Lacks S.A., Mannarelli B.M., Springhorn S.S., Greenberg B.
Cell 46:993-1000(1986) [PubMed: 3019562] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Complete genome sequence of a virulent isolate of Streptococcus pneumoniae."
Tettelin H., Nelson K.E., Paulsen I.T., Eisen J.A., Read T.D., Peterson S.N., Heidelberg J.F., DeBoy R.T., Haft D.H., Dodson R.J., Durkin A.S., Gwinn M.L., Kolonay J.F., Nelson W.C., Peterson J.D., Umayam L.A., White O., Salzberg S.L. expand/collapse author list , Lewis M.R., Radune D., Holtzapple E.K., Khouri H.M., Wolf A.M., Utterback T.R., Hansen C.L., McDonald L.A., Feldblyum T.V., Angiuoli S.V., Dickinson T., Hickey E.K., Holt I.E., Loftus B.J., Yang F., Smith H.O., Venter J.C., Dougherty B.A., Morrison D.A., Hollingshead S.K., Fraser C.M.
Science 293:498-506(2001) [PubMed: 11463916] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-334 / TIGR4.

Cross-references

Sequence databases

M14340 Genomic DNA. Translation: AAA88577.2.
AE005672 Genomic DNA. Translation: AAK75922.1.
PIRA31975.
A95216.
RefSeqNP_346282.1.

3D structure databases

ModBaseSearch...

Protein family/group databases

REBASE776. DpnI.

Genome annotation databases

GeneID930966.
GenomeReviewsGene locus SP_1850 in contig AE005672_GR.
KEGGspn:SP_1850.
TIGRSP_1850.

Phylogenomic databases

HOGENOMP0A459.
OMAQYELKSK.

Enzyme and pathway databases

BRENDA3.1.21.4. 600.

Family and domain databases

InterProIPR010324. DRP.
[Graphical view]
PfamPF06044. DRP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameT2D1_STRPN
AccessionPrimary (citable) accession number: P0A459
Secondary accession number(s): P09356
Entry history
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: November 3, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents