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P0A459

- T2D1_STRPN

UniProt

P0A459 - T2D1_STRPN

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Protein

Type-2 restriction enzyme DpnI

Gene

dpnC

Organism
Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli

Functioni

Recognizes the double-stranded and methylated sequence G(Me)ATC and cleaves after A-2.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

  1. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Enzyme and pathway databases

BioCyciSPNE170187:GHGN-1858-MONOMER.

Protein family/group databases

REBASEi776. DpnI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme DpnI (EC:3.1.21.4)
Short name:
R.DpnI
Alternative name(s):
Endonuclease DpnI
Type II restriction enzyme DpnI
Gene namesi
Name:dpnC
Ordered Locus Names:SP_1850
OrganismiStreptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Taxonomic identifieri170187 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
ProteomesiUP000000585: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 254254Type-2 restriction enzyme DpnIPRO_0000077300Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi170187.SP_1850.

Structurei

Secondary structure

1
254
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi7 – 104Combined sources
Helixi16 – 3116Combined sources
Turni35 – 373Combined sources
Turni57 – 593Combined sources
Beta strandi62 – 709Combined sources
Beta strandi74 – 807Combined sources
Helixi81 – 877Combined sources
Beta strandi95 – 1017Combined sources
Beta strandi105 – 1139Combined sources
Helixi115 – 1173Combined sources
Helixi120 – 1223Combined sources
Beta strandi123 – 1253Combined sources
Turni134 – 1374Combined sources
Beta strandi140 – 1445Combined sources
Helixi150 – 1523Combined sources
Beta strandi154 – 1585Combined sources
Helixi165 – 17511Combined sources
Beta strandi182 – 1843Combined sources
Helixi188 – 19811Combined sources
Beta strandi201 – 2055Combined sources
Helixi206 – 2094Combined sources
Helixi210 – 2123Combined sources
Helixi213 – 2197Combined sources
Turni220 – 2223Combined sources
Helixi226 – 23914Combined sources
Beta strandi242 – 2454Combined sources
Beta strandi247 – 2537Combined sources

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4KYWX-ray2.35A1-254[»]
ProteinModelPortaliP0A459.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiNOG11693.
HOGENOMiHOG000067092.
KOiK01155.
OMAiWIGCNIN.
OrthoDBiEOG67Q982.

Family and domain databases

InterProiIPR010324. DRP.
[Graphical view]
PfamiPF06044. DRP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A459-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MELHFNLELV ETYKSNSQKA RILTEDWVYR QSYCPNCGNN PLNHFENNRP
60 70 80 90 100
VADFYCNHCS EEFELKSKKG NFSSTINDGA YATMMKRVQA DNNPNFFFLT
110 120 130 140 150
YTKNFEVNNF LVLPKQFVTP KSIIQRKPLA PTARRAGWIG CNIDLSQVPS
160 170 180 190 200
KGRIFLVQDG QVRDPEKVTK EFKQGLFLRK SSLSSRGWTI EILNCIDKIE
210 220 230 240 250
GSEFTLEDMY RFESDLKNIF VKNNHIKEKI RQQLQILRDK EIIEFKGRGK

YRKL
Length:254
Mass (Da):29,785
Last modified:March 15, 2005 - v1
Checksum:i7FFFFE14BB3EFEDB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M14340 Genomic DNA. Translation: AAA88577.2.
AE005672 Genomic DNA. Translation: AAK75922.1.
PIRiA31975.
A95216.
RefSeqiNP_346282.1. NC_003028.3.
WP_000418960.1. NZ_AKVY01000001.1.

Genome annotation databases

EnsemblBacteriaiAAK75922; AAK75922; SP_1850.
GeneIDi930966.
KEGGispn:SP_1850.
PATRICi19708161. VBIStrPne105772_1923.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M14340 Genomic DNA. Translation: AAA88577.2 .
AE005672 Genomic DNA. Translation: AAK75922.1 .
PIRi A31975.
A95216.
RefSeqi NP_346282.1. NC_003028.3.
WP_000418960.1. NZ_AKVY01000001.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4KYW X-ray 2.35 A 1-254 [» ]
ProteinModelPortali P0A459.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 170187.SP_1850.

Protein family/group databases

REBASEi 776. DpnI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAK75922 ; AAK75922 ; SP_1850 .
GeneIDi 930966.
KEGGi spn:SP_1850.
PATRICi 19708161. VBIStrPne105772_1923.

Phylogenomic databases

eggNOGi NOG11693.
HOGENOMi HOG000067092.
KOi K01155.
OMAi WIGCNIN.
OrthoDBi EOG67Q982.

Enzyme and pathway databases

BioCyci SPNE170187:GHGN-1858-MONOMER.

Family and domain databases

InterProi IPR010324. DRP.
[Graphical view ]
Pfami PF06044. DRP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Proteins encoded by the DpnI restriction gene cassette. Hyperproduction and characterization of the DpnI endonuclease."
    de la Campa A.G., Springhorn S.S., Kale P., Lacks S.A.
    J. Biol. Chem. 263:14696-14702(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Genetic basis of the complementary DpnI and DpnII restriction systems of S. pneumoniae: an intercellular cassette mechanism."
    Lacks S.A., Mannarelli B.M., Springhorn S.S., Greenberg B.
    Cell 46:993-1000(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-334 / TIGR4.

Entry informationi

Entry nameiT2D1_STRPN
AccessioniPrimary (citable) accession number: P0A459
Secondary accession number(s): P09356
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: October 29, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3