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Protein

Type-2 restriction enzyme DpnI

Gene

dpnC

Organism
Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Recognizes the double-stranded and methylated sequence G(Me)ATC and cleaves after A-2.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Protein family/group databases

REBASEi776. DpnI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme DpnI (EC:3.1.21.4)
Short name:
R.DpnI
Alternative name(s):
Endonuclease DpnI
Type II restriction enzyme DpnI
Gene namesi
Name:dpnC
Ordered Locus Names:SP_1850
OrganismiStreptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Taxonomic identifieri170187 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
Proteomesi
  • UP000000585 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000773001 – 254Type-2 restriction enzyme DpnIAdd BLAST254

Interactioni

Protein-protein interaction databases

STRINGi170187.SP_1850.

Structurei

Secondary structure

1254
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi7 – 10Combined sources4
Helixi16 – 31Combined sources16
Turni35 – 37Combined sources3
Turni57 – 59Combined sources3
Beta strandi62 – 70Combined sources9
Beta strandi74 – 80Combined sources7
Helixi81 – 87Combined sources7
Beta strandi95 – 101Combined sources7
Beta strandi105 – 113Combined sources9
Helixi115 – 117Combined sources3
Helixi120 – 122Combined sources3
Beta strandi123 – 125Combined sources3
Turni134 – 137Combined sources4
Beta strandi140 – 144Combined sources5
Helixi150 – 152Combined sources3
Beta strandi154 – 158Combined sources5
Helixi165 – 175Combined sources11
Beta strandi182 – 184Combined sources3
Helixi188 – 198Combined sources11
Beta strandi201 – 205Combined sources5
Helixi206 – 209Combined sources4
Helixi210 – 212Combined sources3
Helixi213 – 219Combined sources7
Turni220 – 222Combined sources3
Helixi226 – 239Combined sources14
Beta strandi242 – 245Combined sources4
Beta strandi247 – 253Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4KYWX-ray2.35A1-254[»]
ProteinModelPortaliP0A459.
SMRiP0A459.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiENOG4108HED. Bacteria.
ENOG410YTMJ. LUCA.
HOGENOMiHOG000067092.
KOiK01155.
OMAiHHIMCSI.

Family and domain databases

InterProiIPR010324. DRP.
[Graphical view]
PfamiPF06044. DpnI. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A459-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELHFNLELV ETYKSNSQKA RILTEDWVYR QSYCPNCGNN PLNHFENNRP
60 70 80 90 100
VADFYCNHCS EEFELKSKKG NFSSTINDGA YATMMKRVQA DNNPNFFFLT
110 120 130 140 150
YTKNFEVNNF LVLPKQFVTP KSIIQRKPLA PTARRAGWIG CNIDLSQVPS
160 170 180 190 200
KGRIFLVQDG QVRDPEKVTK EFKQGLFLRK SSLSSRGWTI EILNCIDKIE
210 220 230 240 250
GSEFTLEDMY RFESDLKNIF VKNNHIKEKI RQQLQILRDK EIIEFKGRGK

YRKL
Length:254
Mass (Da):29,785
Last modified:March 15, 2005 - v1
Checksum:i7FFFFE14BB3EFEDB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14340 Genomic DNA. Translation: AAA88577.2.
AE005672 Genomic DNA. Translation: AAK75922.1.
PIRiA31975.
A95216.
RefSeqiWP_000418960.1. NZ_AKVY01000001.1.

Genome annotation databases

EnsemblBacteriaiAAK75922; AAK75922; SP_1850.
KEGGispn:SP_1850.
PATRICi19708161. VBIStrPne105772_1923.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14340 Genomic DNA. Translation: AAA88577.2.
AE005672 Genomic DNA. Translation: AAK75922.1.
PIRiA31975.
A95216.
RefSeqiWP_000418960.1. NZ_AKVY01000001.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4KYWX-ray2.35A1-254[»]
ProteinModelPortaliP0A459.
SMRiP0A459.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi170187.SP_1850.

Protein family/group databases

REBASEi776. DpnI.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK75922; AAK75922; SP_1850.
KEGGispn:SP_1850.
PATRICi19708161. VBIStrPne105772_1923.

Phylogenomic databases

eggNOGiENOG4108HED. Bacteria.
ENOG410YTMJ. LUCA.
HOGENOMiHOG000067092.
KOiK01155.
OMAiHHIMCSI.

Family and domain databases

InterProiIPR010324. DRP.
[Graphical view]
PfamiPF06044. DpnI. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiT2D1_STRPN
AccessioniPrimary (citable) accession number: P0A459
Secondary accession number(s): P09356
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: November 2, 2016
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.