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P0A459 (T2D1_STRPN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme DpnI

Short name=R.DpnI
EC=3.1.21.4
Alternative name(s):
Endonuclease DpnI
Type II restriction enzyme DpnI
Gene names
Name:dpnC
Ordered Locus Names:SP_1850
OrganismStreptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) [Complete proteome] [HAMAP]
Taxonomic identifier170187 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Protein attributes

Sequence length254 AA.
Sequence statusComplete.
Protein existencePredicted

General annotation (Comments)

Function

Recognizes the double-stranded and methylated sequence G(Me)ATC and cleaves after A-2.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
   Biological processRestriction system
   Molecular functionEndonuclease
Hydrolase
Nuclease
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionType II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 254254Type-2 restriction enzyme DpnI
PRO_0000077300

Sequences

Sequence LengthMass (Da)Tools
P0A459 [UniParc].

Last modified March 15, 2005. Version 1.
Checksum: 7FFFFE14BB3EFEDB

FASTA25429,785
        10         20         30         40         50         60 
MELHFNLELV ETYKSNSQKA RILTEDWVYR QSYCPNCGNN PLNHFENNRP VADFYCNHCS 

        70         80         90        100        110        120 
EEFELKSKKG NFSSTINDGA YATMMKRVQA DNNPNFFFLT YTKNFEVNNF LVLPKQFVTP 

       130        140        150        160        170        180 
KSIIQRKPLA PTARRAGWIG CNIDLSQVPS KGRIFLVQDG QVRDPEKVTK EFKQGLFLRK 

       190        200        210        220        230        240 
SSLSSRGWTI EILNCIDKIE GSEFTLEDMY RFESDLKNIF VKNNHIKEKI RQQLQILRDK 

       250 
EIIEFKGRGK YRKL 

« Hide

References

« Hide 'large scale' references
[1]"Proteins encoded by the DpnI restriction gene cassette. Hyperproduction and characterization of the DpnI endonuclease."
de la Campa A.G., Springhorn S.S., Kale P., Lacks S.A.
J. Biol. Chem. 263:14696-14702(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Genetic basis of the complementary DpnI and DpnII restriction systems of S. pneumoniae: an intercellular cassette mechanism."
Lacks S.A., Mannarelli B.M., Springhorn S.S., Greenberg B.
Cell 46:993-1000(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Complete genome sequence of a virulent isolate of Streptococcus pneumoniae."
Tettelin H., Nelson K.E., Paulsen I.T., Eisen J.A., Read T.D., Peterson S.N., Heidelberg J.F., DeBoy R.T., Haft D.H., Dodson R.J., Durkin A.S., Gwinn M.L., Kolonay J.F., Nelson W.C., Peterson J.D., Umayam L.A., White O., Salzberg S.L. expand/collapse author list , Lewis M.R., Radune D., Holtzapple E.K., Khouri H.M., Wolf A.M., Utterback T.R., Hansen C.L., McDonald L.A., Feldblyum T.V., Angiuoli S.V., Dickinson T., Hickey E.K., Holt I.E., Loftus B.J., Yang F., Smith H.O., Venter J.C., Dougherty B.A., Morrison D.A., Hollingshead S.K., Fraser C.M.
Science 293:498-506(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-334 / TIGR4.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M14340 Genomic DNA. Translation: AAA88577.2.
AE005672 Genomic DNA. Translation: AAK75922.1.
PIRA31975.
A95216.
RefSeqNP_346282.1. NC_003028.3.

3D structure databases

ProteinModelPortalP0A459.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING170187.SP_1850.

Protein family/group databases

REBASE776. DpnI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK75922; AAK75922; SP_1850.
GeneID930966.
KEGGspn:SP_1850.
PATRIC19708161. VBIStrPne105772_1923.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGNOG11693.
HOGENOMHOG000067092.
KOK01155.
OMARAGWVGC.
OrthoDBEOG67Q982.
ProtClustDBCLSK878276.

Enzyme and pathway databases

BioCycSPNE170187:GHGN-1858-MONOMER.

Family and domain databases

InterProIPR010324. DRP.
[Graphical view]
PfamPF06044. DRP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameT2D1_STRPN
AccessionPrimary (citable) accession number: P0A459
Secondary accession number(s): P09356
Entry history
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: April 16, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries