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P0A459

- T2D1_STRPN

UniProt

P0A459 - T2D1_STRPN

Protein

Type-2 restriction enzyme DpnI

Gene

dpnC

Organism
Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 53 (03 Sep 2014)
      Sequence version 1 (15 Mar 2005)
      Previous versions | rss
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    Functioni

    Recognizes the double-stranded and methylated sequence G(Me)ATC and cleaves after A-2.

    Catalytic activityi

    Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

    GO - Molecular functioni

    1. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    Restriction system

    Enzyme and pathway databases

    BioCyciSPNE170187:GHGN-1858-MONOMER.

    Protein family/group databases

    REBASEi776. DpnI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type-2 restriction enzyme DpnI (EC:3.1.21.4)
    Short name:
    R.DpnI
    Alternative name(s):
    Endonuclease DpnI
    Type II restriction enzyme DpnI
    Gene namesi
    Name:dpnC
    Ordered Locus Names:SP_1850
    OrganismiStreptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
    Taxonomic identifieri170187 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
    ProteomesiUP000000585: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 254254Type-2 restriction enzyme DpnIPRO_0000077300Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi170187.SP_1850.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4KYWX-ray2.35A1-254[»]
    ProteinModelPortaliP0A459.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Phylogenomic databases

    eggNOGiNOG11693.
    HOGENOMiHOG000067092.
    KOiK01155.
    OMAiWIGCNIN.
    OrthoDBiEOG67Q982.

    Family and domain databases

    InterProiIPR010324. DRP.
    [Graphical view]
    PfamiPF06044. DRP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0A459-1 [UniParc]FASTAAdd to Basket

    « Hide

    MELHFNLELV ETYKSNSQKA RILTEDWVYR QSYCPNCGNN PLNHFENNRP    50
    VADFYCNHCS EEFELKSKKG NFSSTINDGA YATMMKRVQA DNNPNFFFLT 100
    YTKNFEVNNF LVLPKQFVTP KSIIQRKPLA PTARRAGWIG CNIDLSQVPS 150
    KGRIFLVQDG QVRDPEKVTK EFKQGLFLRK SSLSSRGWTI EILNCIDKIE 200
    GSEFTLEDMY RFESDLKNIF VKNNHIKEKI RQQLQILRDK EIIEFKGRGK 250
    YRKL 254
    Length:254
    Mass (Da):29,785
    Last modified:March 15, 2005 - v1
    Checksum:i7FFFFE14BB3EFEDB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M14340 Genomic DNA. Translation: AAA88577.2.
    AE005672 Genomic DNA. Translation: AAK75922.1.
    PIRiA31975.
    A95216.
    RefSeqiNP_346282.1. NC_003028.3.
    WP_000418960.1. NZ_AKVY01000001.1.

    Genome annotation databases

    EnsemblBacteriaiAAK75922; AAK75922; SP_1850.
    GeneIDi930966.
    KEGGispn:SP_1850.
    PATRICi19708161. VBIStrPne105772_1923.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M14340 Genomic DNA. Translation: AAA88577.2 .
    AE005672 Genomic DNA. Translation: AAK75922.1 .
    PIRi A31975.
    A95216.
    RefSeqi NP_346282.1. NC_003028.3.
    WP_000418960.1. NZ_AKVY01000001.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4KYW X-ray 2.35 A 1-254 [» ]
    ProteinModelPortali P0A459.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 170187.SP_1850.

    Protein family/group databases

    REBASEi 776. DpnI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAK75922 ; AAK75922 ; SP_1850 .
    GeneIDi 930966.
    KEGGi spn:SP_1850.
    PATRICi 19708161. VBIStrPne105772_1923.

    Phylogenomic databases

    eggNOGi NOG11693.
    HOGENOMi HOG000067092.
    KOi K01155.
    OMAi WIGCNIN.
    OrthoDBi EOG67Q982.

    Enzyme and pathway databases

    BioCyci SPNE170187:GHGN-1858-MONOMER.

    Family and domain databases

    InterProi IPR010324. DRP.
    [Graphical view ]
    Pfami PF06044. DRP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Proteins encoded by the DpnI restriction gene cassette. Hyperproduction and characterization of the DpnI endonuclease."
      de la Campa A.G., Springhorn S.S., Kale P., Lacks S.A.
      J. Biol. Chem. 263:14696-14702(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Genetic basis of the complementary DpnI and DpnII restriction systems of S. pneumoniae: an intercellular cassette mechanism."
      Lacks S.A., Mannarelli B.M., Springhorn S.S., Greenberg B.
      Cell 46:993-1000(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-334 / TIGR4.

    Entry informationi

    Entry nameiT2D1_STRPN
    AccessioniPrimary (citable) accession number: P0A459
    Secondary accession number(s): P09356
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 15, 2005
    Last sequence update: March 15, 2005
    Last modified: September 3, 2014
    This is version 53 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries

    External Data

    Dasty 3