P0A459 (T2D1_STRPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type-2 restriction enzyme DpnI
Short name=R.DpnI
EC=3.1.21.4

Alternative name(s):
Endonuclease DpnI
Type II restriction enzyme DpnI

Gene names

Name:	dpnC
Ordered Locus Names:	SP_1850

Organism

Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) [Complete proteome] [HAMAP]

Taxonomic identifier

170187 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Streptococcus › Streptococcus pneumoniae

Protein attributes

Sequence length	254 AA.
Sequence status	Complete.
Protein existence	Predicted

General annotation (Comments)

Function	Recognizes the double-stranded and methylated sequence G(Me)ATC and cleaves after A-2.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Molecular_function	Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 254	254	Type-2 restriction enzyme DpnI		PRO_0000077300

Sequences

	Sequence		Length	Mass (Da)
	P0A459 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 7FFFFE14BB3EFEDB	FASTA	254	29,785
	10 20 30 40 50 60 MELHFNLELV ETYKSNSQKA RILTEDWVYR QSYCPNCGNN PLNHFENNRP VADFYCNHCS 70 80 90 100 110 120 EEFELKSKKG NFSSTINDGA YATMMKRVQA DNNPNFFFLT YTKNFEVNNF LVLPKQFVTP 130 140 150 160 170 180 KSIIQRKPLA PTARRAGWIG CNIDLSQVPS KGRIFLVQDG QVRDPEKVTK EFKQGLFLRK 190 200 210 220 230 240 SSLSSRGWTI EILNCIDKIE GSEFTLEDMY RFESDLKNIF VKNNHIKEKI RQQLQILRDK 250 EIIEFKGRGK YRKL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Proteins encoded by the DpnI restriction gene cassette. Hyperproduction and characterization of the DpnI endonuclease." de la Campa A.G., Springhorn S.S., Kale P., Lacks S.A. J. Biol. Chem. 263:14696-14702(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Genetic basis of the complementary DpnI and DpnII restriction systems of S. pneumoniae: an intercellular cassette mechanism." Lacks S.A., Mannarelli B.M., Springhorn S.S., Greenberg B. Cell 46:993-1000(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Complete genome sequence of a virulent isolate of Streptococcus pneumoniae." Tettelin H., Nelson K.E., Paulsen I.T., Eisen J.A., Read T.D., Peterson S.N., Heidelberg J.F., DeBoy R.T., Haft D.H., Dodson R.J., Durkin A.S., Gwinn M.L., Kolonay J.F., Nelson W.C., Peterson J.D., Umayam L.A., White O., Salzberg S.L. , Lewis M.R., Radune D., Holtzapple E.K., Khouri H.M., Wolf A.M., Utterback T.R., Hansen C.L., McDonald L.A., Feldblyum T.V., Angiuoli S.V., Dickinson T., Hickey E.K., Holt I.E., Loftus B.J., Yang F., Smith H.O., Venter J.C., Dougherty B.A., Morrison D.A., Hollingshead S.K., Fraser C.M. Science 293:498-506(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-334 / TIGR4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M14340 Genomic DNA. Translation: AAA88577.2. AE005672 Genomic DNA. Translation: AAK75922.1.
PIR	A31975. A95216.
RefSeq	NP_346282.1. NC_003028.3.
3D structure databases
ProteinModelPortal	P0A459.
ModBase	Search...
Protein-protein interaction databases
STRING	170187.SP_1850.
Protein family/group databases
REBASE	776. DpnI.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAK75922; AAK75922; SP_1850.
GeneID	930966.
KEGG	spn:SP_1850.
PATRIC	19708161. VBIStrPne105772_1923.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	NOG11693.
HOGENOM	HOG000067092.
KO	K01155.
OMA	HHIMCSI.
ProtClustDB	CLSK878276.
Family and domain databases
InterPro	IPR010324. DRP. [Graphical view]
Pfam	PF06044. DRP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T2D1_STRPN

Accession

Primary (citable) accession number: P0A459
Secondary accession number(s): P09356

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	May 1, 2013
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program