Reviewed, UniProtKB/Swiss-Prot P0A459 (T2D1_STRPN)

Names and origin

Protein names

Recommended name:
    Type-2 restriction enzyme DpnI
      Short name=R.DpnI
    EC=3.1.21.4
Alternative name(s):
    Type II restriction enzyme DpnI
    Endonuclease DpnI

Gene names

Name:	dpnC
Ordered Locus Names:	SP_1850

Organism

Streptococcus pneumoniae [Complete proteome] [HAMAP]

Taxonomic identifier

1313 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	254 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Predicted.

General annotation (Comments)

Function	Recognizes the double-stranded and methylated sequence G(Me)ATC and cleaves after A-2.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 254	254	Type-2 restriction enzyme DpnI		PRO_0000077300

Sequences

	Sequence		Length	Mass (Da)
	P0A459-1 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 7FFFFE14BB3EFEDB	FASTA	254	29,785
	10 20 30 40 50 60 MELHFNLELV ETYKSNSQKA RILTEDWVYR QSYCPNCGNN PLNHFENNRP VADFYCNHCS 70 80 90 100 110 120 EEFELKSKKG NFSSTINDGA YATMMKRVQA DNNPNFFFLT YTKNFEVNNF LVLPKQFVTP 130 140 150 160 170 180 KSIIQRKPLA PTARRAGWIG CNIDLSQVPS KGRIFLVQDG QVRDPEKVTK EFKQGLFLRK 190 200 210 220 230 240 SSLSSRGWTI EILNCIDKIE GSEFTLEDMY RFESDLKNIF VKNNHIKEKI RQQLQILRDK 250 EIIEFKGRGK YRKL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Proteins encoded by the DpnI restriction gene cassette. Hyperproduction and characterization of the DpnI endonuclease." de la Campa A.G., Springhorn S.S., Kale P., Lacks S.A. J. Biol. Chem. 263:14696-14702(1988) [PubMed: 2844782] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Genetic basis of the complementary DpnI and DpnII restriction systems of S. pneumoniae: an intercellular cassette mechanism." Lacks S.A., Mannarelli B.M., Springhorn S.S., Greenberg B. Cell 46:993-1000(1986) [PubMed: 3019562] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Complete genome sequence of a virulent isolate of Streptococcus pneumoniae." Tettelin H., Nelson K.E., Paulsen I.T., Eisen J.A., Read T.D., Peterson S.N., Heidelberg J.F., DeBoy R.T., Haft D.H., Dodson R.J., Durkin A.S., Gwinn M.L., Kolonay J.F., Nelson W.C., Peterson J.D., Umayam L.A., White O., Salzberg S.L. , Lewis M.R., Radune D., Holtzapple E.K., Khouri H.M., Wolf A.M., Utterback T.R., Hansen C.L., McDonald L.A., Feldblyum T.V., Angiuoli S.V., Dickinson T., Hickey E.K., Holt I.E., Loftus B.J., Yang F., Smith H.O., Venter J.C., Dougherty B.A., Morrison D.A., Hollingshead S.K., Fraser C.M. Science 293:498-506(2001) [PubMed: 11463916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-334 / TIGR4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M14340 Genomic DNA. Translation: AAA88577.2. AE005672 Genomic DNA. Translation: AAK75922.1.
PIR	A31975. A95216.
RefSeq	NP_346282.1.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	776. DpnI.
Genome annotation databases
GeneID	930966.
GenomeReviews	Gene locus SP_1850 in contig AE005672_GR.
KEGG	spn:SP_1850.
TIGR	SP_1850.
Phylogenomic databases
HOGENOM	P0A459.
OMA	QYELKSK.
Enzyme and pathway databases
BRENDA	3.1.21.4. 600.
Family and domain databases
InterPro	IPR010324. DRP. [Graphical view]
Pfam	PF06044. DRP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T2D1_STRPN

Accession

Primary (citable) accession number: P0A459
Secondary accession number(s): P09356

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	November 3, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)