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P0A458

- T2A1_ANAVA

UniProt

P0A458 - T2A1_ANAVA

Protein

Type-2 restriction enzyme AvaI

Gene

avaIR

Organism
Anabaena variabilis
Status
Reviewed - Annotation score: 2 out of 5- Protein predictedi
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    • History
      Entry version 34 (11 Jun 2014)
      Sequence version 1 (15 Mar 2005)
      Previous versions | rss
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    Functioni

    Recognizes the double-stranded sequence CYCGRG and cleaves after C-1.

    Catalytic activityi

    Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    Restriction system

    Protein family/group databases

    REBASEi165. AvaI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type-2 restriction enzyme AvaI (EC:3.1.21.4)
    Short name:
    R.AvaI
    Alternative name(s):
    Endonuclease AvaI
    Type II restriction enzyme AvaI
    Gene namesi
    Name:avaIR
    OrganismiAnabaena variabilis
    Taxonomic identifieri1172 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaNostocalesNostocaceaeAnabaena

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 315315Type-2 restriction enzyme AvaIPRO_0000077280Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP0A458.
    SMRiP0A458. Positions 1-315.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Family and domain databases

    Gene3Di3.40.91.10. 2 hits.
    InterProiIPR011335. Restrct_endonuc-II-like.
    IPR015277. Restrct_endonuc_II_AvaI/BsoBI.
    IPR011544. Restrct_endonuc_II_Cfr10/BsoB1.
    [Graphical view]
    PfamiPF09194. Endonuc-BsobI. 1 hit.
    [Graphical view]
    ProDomiPD040871. Restrict_endonuc_II_AvaI/BsoBI. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SUPFAMiSSF52980. SSF52980. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P0A458-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPYQYHIQSN DDLVTPYQEV RAGFVALALE RNRKATPFVE QARALKIRVS    50
    QIERPQDLLQ MRDIRPTLLA ASGVSDKAAG HLQEQDKVDA IEGLIQNFLE 100
    PAGENFVEEL VYRFLLTRGD TLGGSMRNVG GILAERKFAR YIISALTLSN 150
    TSYKWLDKNS KTWLNQPDDD TDIELRLRGL SWNLEGRNRT FIYNVNVPIV 200
    RKNIDICLFD CRQNEIEKNI ISNPNIYIAL GELKGGIDPA GADEHWKTAN 250
    SALARIRTAF DRHSLKPYTF FVGSAIEKSM AEEIWHQLNS GILTNAANLT 300
    QPDQVASLCA WFIQL 315
    Length:315
    Mass (Da):35,679
    Last modified:March 15, 2005 - v1
    Checksum:i6F22C7CE4D329E33
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X98339 Genomic DNA. Translation: CAA66985.1.
    PIRiS72472.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X98339 Genomic DNA. Translation: CAA66985.1 .
    PIRi S72472.

    3D structure databases

    ProteinModelPortali P0A458.
    SMRi P0A458. Positions 1-315.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 165. AvaI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.91.10. 2 hits.
    InterProi IPR011335. Restrct_endonuc-II-like.
    IPR015277. Restrct_endonuc_II_AvaI/BsoBI.
    IPR011544. Restrct_endonuc_II_Cfr10/BsoB1.
    [Graphical view ]
    Pfami PF09194. Endonuc-BsobI. 1 hit.
    [Graphical view ]
    ProDomi PD040871. Restrict_endonuc_II_AvaI/BsoBI. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SUPFAMi SSF52980. SSF52980. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequence comparison of AvaI and BsoBI restriction-modification systems."
      Ruan H., Lunnen K.D., Scott M.E., Moran L.S., Slatko B.E., Pelletier J.J., Hess E.J., Benner J. II, Wilson G.G., Xu S.-Y.
      Mol. Gen. Genet. 252:695-699(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: PCC 7118 / ATCC 27892.

    Entry informationi

    Entry nameiT2A1_ANAVA
    AccessioniPrimary (citable) accession number: P0A458
    Secondary accession number(s): P70803
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 15, 2005
    Last sequence update: March 15, 2005
    Last modified: June 11, 2014
    This is version 34 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries

    External Data

    Dasty 3