Reviewed, UniProtKB/Swiss-Prot P0A458 (T2A1_ANAVA)

Names and origin

Protein names

Recommended name:
    Type-2 restriction enzyme AvaI
      Short name=R.AvaI
    EC=3.1.21.4
Alternative name(s):
    Type II restriction enzyme AvaI
    Endonuclease AvaI

Gene names

Name:

avaIR

Organism

Anabaena variabilis

Taxonomic identifier

1172 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Anabaena

Protein attributes

Sequence length	315 AA.
Sequence status	Complete.
Protein existence	Predicted.

General annotation (Comments)

Function	Recognizes the double-stranded sequence CYCGRG and cleaves after C-1.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Gene Ontology (GO)
Biological process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 315	315	Type-2 restriction enzyme AvaI		PRO_0000077280

Sequences

	Sequence		Length	Mass (Da)
	P0A458-1 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 6F22C7CE4D329E33	FASTA	315	35,679
	10 20 30 40 50 60 MPYQYHIQSN DDLVTPYQEV RAGFVALALE RNRKATPFVE QARALKIRVS QIERPQDLLQ 70 80 90 100 110 120 MRDIRPTLLA ASGVSDKAAG HLQEQDKVDA IEGLIQNFLE PAGENFVEEL VYRFLLTRGD 130 140 150 160 170 180 TLGGSMRNVG GILAERKFAR YIISALTLSN TSYKWLDKNS KTWLNQPDDD TDIELRLRGL 190 200 210 220 230 240 SWNLEGRNRT FIYNVNVPIV RKNIDICLFD CRQNEIEKNI ISNPNIYIAL GELKGGIDPA 250 260 270 280 290 300 GADEHWKTAN SALARIRTAF DRHSLKPYTF FVGSAIEKSM AEEIWHQLNS GILTNAANLT 310 QPDQVASLCA WFIQL

References

[1]

"Cloning and sequence comparison of AvaI and BsoBI restriction-modification systems." Ruan H., Lunnen K.D., Scott M.E., Moran L.S., Slatko B.E., Pelletier J.J., Hess E.J., Benner J. II, Wilson G.G., Xu S.-Y. Mol. Gen. Genet. 252:695-699(1996) [PubMed: 8917312] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: PCC 7118 / ATCC 27892.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X98339 Genomic DNA. Translation: CAA66985.1.
PIR	S72472.
3D structure databases
SMR	P0A458. Positions 1-315.
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.1.21.4. 2836.
Family and domain databases
InterPro	IPR011544. Restrct_endonuc_II_Cfr10/BsoB1. IPR011335. Restrict_endonuc_II-like_core. IPR015277. Restrict_endonuc_II_AvaI/BsoBI. [Graphical view]
Gene3D	G3DSA:3.40.91.10. Restrct_endonuc_II_Cfr10/BsoB1. 1 hit.
Pfam	PF09194. Endonuc-BsobI. 1 hit. [Graphical view]
ProDom	PD040871. Restrict_endonuc_II_AvaI/BsoBI. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

T2A1_ANAVA

Accession

Primary (citable) accession number: P0A458
Secondary accession number(s): P70803

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	January 19, 2010
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)