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P0A457

- T2A1_NOSS1

UniProt

P0A457 - T2A1_NOSS1

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Protein

Type-2 restriction enzyme AvaI

Gene

avaIR

Organism
Nostoc sp. (strain PCC 7120 / UTEX 2576)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

Recognizes the double-stranded sequence CYCGRG and cleaves after C-1.By similarity

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Protein family/group databases

REBASEi165. AvaI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme AvaI (EC:3.1.21.4)
Short name:
R.AvaI
Alternative name(s):
Endonuclease AvaI
Type II restriction enzyme AvaI
Gene namesi
Name:avaIR
Ordered Locus Names:all3631
OrganismiNostoc sp. (strain PCC 7120 / UTEX 2576)
Taxonomic identifieri103690 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaNostocalesNostocaceaeNostoc
ProteomesiUP000002483: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 315315Type-2 restriction enzyme AvaIPRO_0000077279Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi103690.all3631.

Structurei

3D structure databases

ProteinModelPortaliP0A457.
SMRiP0A457. Positions 1-315.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiNOG87055.
HOGENOMiHOG000005547.
KOiK01155.
OMAiAGADEHW.
OrthoDBiEOG6CGC9V.

Family and domain databases

Gene3Di3.40.91.10. 2 hits.
InterProiIPR011335. Restrct_endonuc-II-like.
IPR015277. Restrct_endonuc_II_AvaI/BsoBI.
IPR011544. Restrct_endonuc_II_Cfr10/BsoB1.
[Graphical view]
PfamiPF09194. Endonuc-BsobI. 1 hit.
[Graphical view]
ProDomiPD040871. Restrict_endonuc_II_AvaI/BsoBI. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF52980. SSF52980. 1 hit.

Sequencei

Sequence statusi: Complete.

P0A457-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPYQYHIQSN DDLVTPYQEV RAGFVALALE RNRKATPFVE QARALKIRVS
60 70 80 90 100
QIERPQDLLQ MRDIRPTLLA ASGVSDKAAG HLQEQDKVDA IEGLIQNFLE
110 120 130 140 150
PAGENFVEEL VYRFLLTRGD TLGGSMRNVG GILAERKFAR YIISALTLSN
160 170 180 190 200
TSYKWLDKNS KTWLNQPDDD TDIELRLRGL SWNLEGRNRT FIYNVNVPIV
210 220 230 240 250
RKNIDICLFD CRQNEIEKNI ISNPNIYIAL GELKGGIDPA GADEHWKTAN
260 270 280 290 300
SALARIRTAF DRHSLKPYTF FVGSAIEKSM AEEIWHQLNS GILTNAANLT
310
QPDQVASLCA WFIQL
Length:315
Mass (Da):35,679
Last modified:March 15, 2005 - v1
Checksum:i6F22C7CE4D329E33
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000019 Genomic DNA. Translation: BAB75330.1.
PIRiAH2259.
RefSeqiNP_487671.1. NC_003272.1.
WP_010997776.1. NC_003272.1.

Genome annotation databases

EnsemblBacteriaiBAB75330; BAB75330; BAB75330.
GeneIDi1107229.
KEGGiana:all3631.
PATRICi22777712. VBINosSp37423_4310.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000019 Genomic DNA. Translation: BAB75330.1 .
PIRi AH2259.
RefSeqi NP_487671.1. NC_003272.1.
WP_010997776.1. NC_003272.1.

3D structure databases

ProteinModelPortali P0A457.
SMRi P0A457. Positions 1-315.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 103690.all3631.

Protein family/group databases

REBASEi 165. AvaI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB75330 ; BAB75330 ; BAB75330 .
GeneIDi 1107229.
KEGGi ana:all3631.
PATRICi 22777712. VBINosSp37423_4310.

Phylogenomic databases

eggNOGi NOG87055.
HOGENOMi HOG000005547.
KOi K01155.
OMAi AGADEHW.
OrthoDBi EOG6CGC9V.

Family and domain databases

Gene3Di 3.40.91.10. 2 hits.
InterProi IPR011335. Restrct_endonuc-II-like.
IPR015277. Restrct_endonuc_II_AvaI/BsoBI.
IPR011544. Restrct_endonuc_II_Cfr10/BsoB1.
[Graphical view ]
Pfami PF09194. Endonuc-BsobI. 1 hit.
[Graphical view ]
ProDomi PD040871. Restrict_endonuc_II_AvaI/BsoBI. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SUPFAMi SSF52980. SSF52980. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 7120 / UTEX 2576.

Entry informationi

Entry nameiT2A1_NOSS1
AccessioniPrimary (citable) accession number: P0A457
Secondary accession number(s): P70803
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: October 1, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3