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Protein

Type-2 restriction enzyme AvaI

Gene

avaIR

Organism
Nostoc sp. (strain PCC 7120 / UTEX 2576)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Recognizes the double-stranded sequence CYCGRG and cleaves after C-1.By similarity

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Protein family/group databases

REBASEi165. AvaI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme AvaI (EC:3.1.21.4)
Short name:
R.AvaI
Alternative name(s):
Endonuclease AvaI
Type II restriction enzyme AvaI
Gene namesi
Name:avaIR
Ordered Locus Names:all3631
OrganismiNostoc sp. (strain PCC 7120 / UTEX 2576)
Taxonomic identifieri103690 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaNostocalesNostocaceaeNostoc
ProteomesiUP000002483 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 315315Type-2 restriction enzyme AvaIPRO_0000077279Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi103690.all3631.

Structurei

3D structure databases

ProteinModelPortaliP0A457.
SMRiP0A457. Positions 1-315.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiNOG87055.
HOGENOMiHOG000005547.
KOiK01155.
OMAiAGADEHW.
OrthoDBiEOG6CGC9V.

Family and domain databases

Gene3Di3.40.91.10. 2 hits.
InterProiIPR011335. Restrct_endonuc-II-like.
IPR015277. Restrct_endonuc_II_AvaI/BsoBI.
IPR011544. Restrct_endonuc_II_Cfr10/BsoB1.
[Graphical view]
PfamiPF09194. Endonuc-BsobI. 1 hit.
[Graphical view]
ProDomiPD040871. Restrict_endonuc_II_AvaI/BsoBI. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF52980. SSF52980. 1 hit.

Sequencei

Sequence statusi: Complete.

P0A457-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPYQYHIQSN DDLVTPYQEV RAGFVALALE RNRKATPFVE QARALKIRVS
60 70 80 90 100
QIERPQDLLQ MRDIRPTLLA ASGVSDKAAG HLQEQDKVDA IEGLIQNFLE
110 120 130 140 150
PAGENFVEEL VYRFLLTRGD TLGGSMRNVG GILAERKFAR YIISALTLSN
160 170 180 190 200
TSYKWLDKNS KTWLNQPDDD TDIELRLRGL SWNLEGRNRT FIYNVNVPIV
210 220 230 240 250
RKNIDICLFD CRQNEIEKNI ISNPNIYIAL GELKGGIDPA GADEHWKTAN
260 270 280 290 300
SALARIRTAF DRHSLKPYTF FVGSAIEKSM AEEIWHQLNS GILTNAANLT
310
QPDQVASLCA WFIQL
Length:315
Mass (Da):35,679
Last modified:March 15, 2005 - v1
Checksum:i6F22C7CE4D329E33
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000019 Genomic DNA. Translation: BAB75330.1.
PIRiAH2259.
RefSeqiNP_487671.1. NC_003272.1.
WP_010997776.1. NC_003272.1.

Genome annotation databases

EnsemblBacteriaiBAB75330; BAB75330; BAB75330.
KEGGiana:all3631.
PATRICi22777712. VBINosSp37423_4310.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000019 Genomic DNA. Translation: BAB75330.1.
PIRiAH2259.
RefSeqiNP_487671.1. NC_003272.1.
WP_010997776.1. NC_003272.1.

3D structure databases

ProteinModelPortaliP0A457.
SMRiP0A457. Positions 1-315.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi103690.all3631.

Protein family/group databases

REBASEi165. AvaI.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB75330; BAB75330; BAB75330.
KEGGiana:all3631.
PATRICi22777712. VBINosSp37423_4310.

Phylogenomic databases

eggNOGiNOG87055.
HOGENOMiHOG000005547.
KOiK01155.
OMAiAGADEHW.
OrthoDBiEOG6CGC9V.

Family and domain databases

Gene3Di3.40.91.10. 2 hits.
InterProiIPR011335. Restrct_endonuc-II-like.
IPR015277. Restrct_endonuc_II_AvaI/BsoBI.
IPR011544. Restrct_endonuc_II_Cfr10/BsoB1.
[Graphical view]
PfamiPF09194. Endonuc-BsobI. 1 hit.
[Graphical view]
ProDomiPD040871. Restrict_endonuc_II_AvaI/BsoBI. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF52980. SSF52980. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 7120 / UTEX 2576.

Entry informationi

Entry nameiT2A1_NOSS1
AccessioniPrimary (citable) accession number: P0A457
Secondary accession number(s): P70803
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: April 29, 2015
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.