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P0A457 (T2A1_NOSS1) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme AvaI

Short name=R.AvaI
EC=3.1.21.4
Alternative name(s):
Endonuclease AvaI
Type II restriction enzyme AvaI
Gene names
Name:avaIR
Ordered Locus Names:all3631
OrganismNostoc sp. (strain PCC 7120 / UTEX 2576) [Complete proteome] [HAMAP]
Taxonomic identifier103690 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaNostocalesNostocaceaeNostoc

Protein attributes

Sequence length315 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Recognizes the double-stranded sequence CYCGRG and cleaves after C-1 By similarity.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
   Biological processRestriction system
   Molecular functionEndonuclease
Hydrolase
Nuclease
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: InterPro

Type II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 315315Type-2 restriction enzyme AvaI
PRO_0000077279

Sequences

Sequence LengthMass (Da)Tools
P0A457 [UniParc].

Last modified March 15, 2005. Version 1.
Checksum: 6F22C7CE4D329E33

FASTA31535,679
        10         20         30         40         50         60 
MPYQYHIQSN DDLVTPYQEV RAGFVALALE RNRKATPFVE QARALKIRVS QIERPQDLLQ 

        70         80         90        100        110        120 
MRDIRPTLLA ASGVSDKAAG HLQEQDKVDA IEGLIQNFLE PAGENFVEEL VYRFLLTRGD 

       130        140        150        160        170        180 
TLGGSMRNVG GILAERKFAR YIISALTLSN TSYKWLDKNS KTWLNQPDDD TDIELRLRGL 

       190        200        210        220        230        240 
SWNLEGRNRT FIYNVNVPIV RKNIDICLFD CRQNEIEKNI ISNPNIYIAL GELKGGIDPA 

       250        260        270        280        290        300 
GADEHWKTAN SALARIRTAF DRHSLKPYTF FVGSAIEKSM AEEIWHQLNS GILTNAANLT 

       310 
QPDQVASLCA WFIQL 

« Hide

References

[1]"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120."
Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. expand/collapse author list , Takazawa M., Yamada M., Yasuda M., Tabata S.
DNA Res. 8:205-213(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PCC 7120 / UTEX 2576.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000019 Genomic DNA. Translation: BAB75330.1.
PIRAH2259.
RefSeqNP_487671.1. NC_003272.1.

3D structure databases

ProteinModelPortalP0A457.
SMRP0A457. Positions 1-315.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING103690.all3631.

Protein family/group databases

REBASE165. AvaI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB75330; BAB75330; BAB75330.
GeneID1107229.
KEGGana:all3631.
PATRIC22777712. VBINosSp37423_4310.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGNOG87055.
HOGENOMHOG000005547.
KOK01155.
OMAAGADEHW.
OrthoDBEOG6CGC9V.
ProtClustDBCLSK896010.

Family and domain databases

Gene3D3.40.91.10. 2 hits.
InterProIPR011335. Restrct_endonuc-II-like.
IPR015277. Restrct_endonuc_II_AvaI/BsoBI.
IPR011544. Restrct_endonuc_II_Cfr10/BsoB1.
[Graphical view]
PfamPF09194. Endonuc-BsobI. 1 hit.
[Graphical view]
ProDomPD040871. Restrict_endonuc_II_AvaI/BsoBI. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMSSF52980. SSF52980. 1 hit.
ProtoNetSearch...

Entry information

Entry nameT2A1_NOSS1
AccessionPrimary (citable) accession number: P0A457
Secondary accession number(s): P70803
Entry history
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: April 16, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries