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P0A457

- T2A1_NOSS1

UniProt

P0A457 - T2A1_NOSS1

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Protein
Type-2 restriction enzyme AvaI
Gene
avaIR, all3631
Organism
Nostoc sp. (strain PCC 7120 / UTEX 2576)
Status
Reviewed - Annotation score: 2 out of 5 - Protein inferred from homologyi

Functioni

Recognizes the double-stranded sequence CYCGRG and cleaves after C-1 By similarity.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Protein family/group databases

REBASEi165. AvaI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme AvaI (EC:3.1.21.4)
Short name:
R.AvaI
Alternative name(s):
Endonuclease AvaI
Type II restriction enzyme AvaI
Gene namesi
Name:avaIR
Ordered Locus Names:all3631
OrganismiNostoc sp. (strain PCC 7120 / UTEX 2576)
Taxonomic identifieri103690 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaNostocalesNostocaceaeNostoc
ProteomesiUP000002483: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 315315Type-2 restriction enzyme AvaI
PRO_0000077279Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi103690.all3631.

Structurei

3D structure databases

ProteinModelPortaliP0A457.
SMRiP0A457. Positions 1-315.

Family & Domainsi

Phylogenomic databases

eggNOGiNOG87055.
HOGENOMiHOG000005547.
KOiK01155.
OMAiAGADEHW.
OrthoDBiEOG6CGC9V.

Family and domain databases

Gene3Di3.40.91.10. 2 hits.
InterProiIPR011335. Restrct_endonuc-II-like.
IPR015277. Restrct_endonuc_II_AvaI/BsoBI.
IPR011544. Restrct_endonuc_II_Cfr10/BsoB1.
[Graphical view]
PfamiPF09194. Endonuc-BsobI. 1 hit.
[Graphical view]
ProDomiPD040871. Restrict_endonuc_II_AvaI/BsoBI. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF52980. SSF52980. 1 hit.

Sequencei

Sequence statusi: Complete.

P0A457-1 [UniParc]FASTAAdd to Basket

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MPYQYHIQSN DDLVTPYQEV RAGFVALALE RNRKATPFVE QARALKIRVS    50
QIERPQDLLQ MRDIRPTLLA ASGVSDKAAG HLQEQDKVDA IEGLIQNFLE 100
PAGENFVEEL VYRFLLTRGD TLGGSMRNVG GILAERKFAR YIISALTLSN 150
TSYKWLDKNS KTWLNQPDDD TDIELRLRGL SWNLEGRNRT FIYNVNVPIV 200
RKNIDICLFD CRQNEIEKNI ISNPNIYIAL GELKGGIDPA GADEHWKTAN 250
SALARIRTAF DRHSLKPYTF FVGSAIEKSM AEEIWHQLNS GILTNAANLT 300
QPDQVASLCA WFIQL 315
Length:315
Mass (Da):35,679
Last modified:March 15, 2005 - v1
Checksum:i6F22C7CE4D329E33
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000019 Genomic DNA. Translation: BAB75330.1.
PIRiAH2259.
RefSeqiNP_487671.1. NC_003272.1.
WP_010997776.1. NC_003272.1.

Genome annotation databases

EnsemblBacteriaiBAB75330; BAB75330; BAB75330.
GeneIDi1107229.
KEGGiana:all3631.
PATRICi22777712. VBINosSp37423_4310.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000019 Genomic DNA. Translation: BAB75330.1 .
PIRi AH2259.
RefSeqi NP_487671.1. NC_003272.1.
WP_010997776.1. NC_003272.1.

3D structure databases

ProteinModelPortali P0A457.
SMRi P0A457. Positions 1-315.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 103690.all3631.

Protein family/group databases

REBASEi 165. AvaI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB75330 ; BAB75330 ; BAB75330 .
GeneIDi 1107229.
KEGGi ana:all3631.
PATRICi 22777712. VBINosSp37423_4310.

Phylogenomic databases

eggNOGi NOG87055.
HOGENOMi HOG000005547.
KOi K01155.
OMAi AGADEHW.
OrthoDBi EOG6CGC9V.

Family and domain databases

Gene3Di 3.40.91.10. 2 hits.
InterProi IPR011335. Restrct_endonuc-II-like.
IPR015277. Restrct_endonuc_II_AvaI/BsoBI.
IPR011544. Restrct_endonuc_II_Cfr10/BsoB1.
[Graphical view ]
Pfami PF09194. Endonuc-BsobI. 1 hit.
[Graphical view ]
ProDomi PD040871. Restrict_endonuc_II_AvaI/BsoBI. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SUPFAMi SSF52980. SSF52980. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 7120 / UTEX 2576.

Entry informationi

Entry nameiT2A1_NOSS1
AccessioniPrimary (citable) accession number: P0A457
Secondary accession number(s): P70803
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: September 3, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3

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