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P0A443 (PFLA_LISIN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pyruvate formate-lyase-activating enzyme

Short name=PFL-activating enzyme
EC=1.97.1.4
Gene names
Name:pflA
Synonyms:pflC
Ordered Locus Names:lin1444
OrganismListeria innocua serovar 6a (strain CLIP 11262) [Complete proteome] [HAMAP]
Taxonomic identifier272626 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria

Protein attributes

Sequence length248 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Activation of pyruvate formate-lyase under anaerobic conditions by generation of an organic free radical, using S-adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine By similarity.

Catalytic activity

S-adenosyl-L-methionine + dihydroflavodoxin + [formate C-acetyltransferase]-glycine = 5'-deoxyadenosine + L-methionine + flavodoxin semiquinone + [formate C-acetyltransferase]-glycin-2-yl radical.

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the organic radical-activating enzymes family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 248248Pyruvate formate-lyase-activating enzyme
PRO_0000200530

Sites

Metal binding311Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal binding351Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal binding381Iron-sulfur (4Fe-4S-S-AdoMet) By similarity

Sequences

Sequence LengthMass (Da)Tools
P0A443 [UniParc].

Last modified March 15, 2005. Version 1.
Checksum: 0A6F5BBA95CBEBC9

FASTA24828,115
        10         20         30         40         50         60 
MTEVLGRVHS VETMGTVDGP GIRFIVFMQG CLLRCQFCHN PDTWKIGTGT ERSAQDVFDE 

        70         80         90        100        110        120 
AIKYKEFWDA SGGGVTVSGG EPLLQVDFLI EFFTLCKAAG VHTTIDSCGG CFTRDPEFIE 

       130        140        150        160        170        180 
KLDRLMEVTD LILLDIKQIN PEKHLKLTTK SNAPIIDFAH YLRDKEQPIW IRHVLIPTKT 

       190        200        210        220        230        240 
DDPEDLTKLH EFIQTLPNVK QVDVLPYHTM GVYKWKEMGI RYPLEGIEAP EEEVVALANK 


ILETSSYK 

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL596168 Genomic DNA. Translation: CAC96675.1.
PIRAC1613.
RefSeqNP_470780.1. NC_003212.1.

3D structure databases

ProteinModelPortalP0A443.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272626.lin1444.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAC96675; CAC96675; CAC96675.
GeneID1130045.
KEGGlin:lin1444.
PATRIC20299621. VBILisInn102668_1477.

Organism-specific databases

GenoListLIN1444.
CMRSearch...

Phylogenomic databases

eggNOGCOG1180.
HOGENOMHOG000011458.
KOK04069.
OMAWEMETNK.
OrthoDBEOG64FKHC.

Family and domain databases

InterProIPR012838. PFL_activating.
IPR001989. Radical_activat_CS.
IPR007197. rSAM.
[Graphical view]
PfamPF04055. Radical_SAM. 1 hit.
[Graphical view]
TIGRFAMsTIGR02493. PFLA. 1 hit.
PROSITEPS01087. RADICAL_ACTIVATING. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePFLA_LISIN
AccessionPrimary (citable) accession number: P0A443
Secondary accession number(s): Q9X767
Entry history
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: May 14, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families