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P0A443

- PFLA_LISIN

UniProt

P0A443 - PFLA_LISIN

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Protein

Pyruvate formate-lyase-activating enzyme

Gene
pflA, pflC, lin1444
Organism
Listeria innocua serovar 6a (strain CLIP 11262)
Status
Reviewed - Annotation score: 2 out of 5 - Protein inferred from homologyi

Functioni

Activation of pyruvate formate-lyase under anaerobic conditions by generation of an organic free radical, using S-adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine By similarity.

Catalytic activityi

S-adenosyl-L-methionine + dihydroflavodoxin + [formate C-acetyltransferase]-glycine = 5'-deoxyadenosine + L-methionine + flavodoxin semiquinone + [formate C-acetyltransferase]-glycin-2-yl radical.

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi31 – 311Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal bindingi35 – 351Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal bindingi38 – 381Iron-sulfur (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. [formate-C-acetyltransferase]-activating enzyme activity Source: UniProtKB-EC
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate formate-lyase-activating enzyme (EC:1.97.1.4)
Short name:
PFL-activating enzyme
Gene namesi
Name:pflA
Synonyms:pflC
Ordered Locus Names:lin1444
OrganismiListeria innocua serovar 6a (strain CLIP 11262)
Taxonomic identifieri272626 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria
ProteomesiUP000002513: Chromosome

Organism-specific databases

GenoListiLIN1444.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 248248Pyruvate formate-lyase-activating enzymePRO_0000200530Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272626.lin1444.

Structurei

3D structure databases

ProteinModelPortaliP0A443.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1180.
HOGENOMiHOG000011458.
KOiK04069.
OMAiWEMETNK.
OrthoDBiEOG64FKHC.

Family and domain databases

InterProiIPR012838. PFL_activating.
IPR001989. Radical_activat_CS.
IPR007197. rSAM.
[Graphical view]
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02493. PFLA. 1 hit.
PROSITEiPS01087. RADICAL_ACTIVATING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A443-1 [UniParc]FASTAAdd to Basket

« Hide

MTEVLGRVHS VETMGTVDGP GIRFIVFMQG CLLRCQFCHN PDTWKIGTGT    50
ERSAQDVFDE AIKYKEFWDA SGGGVTVSGG EPLLQVDFLI EFFTLCKAAG 100
VHTTIDSCGG CFTRDPEFIE KLDRLMEVTD LILLDIKQIN PEKHLKLTTK 150
SNAPIIDFAH YLRDKEQPIW IRHVLIPTKT DDPEDLTKLH EFIQTLPNVK 200
QVDVLPYHTM GVYKWKEMGI RYPLEGIEAP EEEVVALANK ILETSSYK 248
Length:248
Mass (Da):28,115
Last modified:March 15, 2005 - v1
Checksum:i0A6F5BBA95CBEBC9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL596168 Genomic DNA. Translation: CAC96675.1.
PIRiAC1613.
RefSeqiNP_470780.1. NC_003212.1.

Genome annotation databases

EnsemblBacteriaiCAC96675; CAC96675; CAC96675.
GeneIDi1130045.
KEGGilin:lin1444.
PATRICi20299621. VBILisInn102668_1477.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL596168 Genomic DNA. Translation: CAC96675.1 .
PIRi AC1613.
RefSeqi NP_470780.1. NC_003212.1.

3D structure databases

ProteinModelPortali P0A443.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272626.lin1444.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAC96675 ; CAC96675 ; CAC96675 .
GeneIDi 1130045.
KEGGi lin:lin1444.
PATRICi 20299621. VBILisInn102668_1477.

Organism-specific databases

GenoListi LIN1444.

Phylogenomic databases

eggNOGi COG1180.
HOGENOMi HOG000011458.
KOi K04069.
OMAi WEMETNK.
OrthoDBi EOG64FKHC.

Family and domain databases

InterProi IPR012838. PFL_activating.
IPR001989. Radical_activat_CS.
IPR007197. rSAM.
[Graphical view ]
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR02493. PFLA. 1 hit.
PROSITEi PS01087. RADICAL_ACTIVATING. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CLIP 11262.

Entry informationi

Entry nameiPFLA_LISIN
AccessioniPrimary (citable) accession number: P0A443
Secondary accession number(s): Q9X767
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: May 14, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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