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Protein

Pyruvate formate-lyase-activating enzyme

Gene

pflA

Organism
Listeria innocua serovar 6a (strain CLIP 11262)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Activation of pyruvate formate-lyase under anaerobic conditions by generation of an organic free radical, using S-adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine.By similarity

Catalytic activityi

S-adenosyl-L-methionine + dihydroflavodoxin + [formate C-acetyltransferase]-glycine = 5'-deoxyadenosine + L-methionine + flavodoxin semiquinone + [formate C-acetyltransferase]-glycin-2-yl radical.

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi31 – 311Iron-sulfur (4Fe-4S-S-AdoMet)By similarity
Metal bindingi35 – 351Iron-sulfur (4Fe-4S-S-AdoMet)By similarity
Metal bindingi38 – 381Iron-sulfur (4Fe-4S-S-AdoMet)By similarity

GO - Molecular functioni

  1. [formate-C-acetyltransferase]-activating enzyme activity Source: UniProtKB-EC
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate formate-lyase-activating enzyme (EC:1.97.1.4)
Short name:
PFL-activating enzyme
Gene namesi
Name:pflA
Synonyms:pflC
Ordered Locus Names:lin1444
OrganismiListeria innocua serovar 6a (strain CLIP 11262)
Taxonomic identifieri272626 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria
ProteomesiUP000002513: Chromosome

Organism-specific databases

GenoListiLIN1444.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 248248Pyruvate formate-lyase-activating enzymePRO_0000200530Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272626.lin1444.

Structurei

3D structure databases

ProteinModelPortaliP0A443.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1180.
HOGENOMiHOG000011458.
KOiK04069.
OMAiRFVLFMQ.
OrthoDBiEOG64FKHC.

Family and domain databases

InterProiIPR012838. PFL_activating.
IPR001989. Radical_activat_CS.
IPR007197. rSAM.
[Graphical view]
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02493. PFLA. 1 hit.
PROSITEiPS01087. RADICAL_ACTIVATING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A443-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTEVLGRVHS VETMGTVDGP GIRFIVFMQG CLLRCQFCHN PDTWKIGTGT
60 70 80 90 100
ERSAQDVFDE AIKYKEFWDA SGGGVTVSGG EPLLQVDFLI EFFTLCKAAG
110 120 130 140 150
VHTTIDSCGG CFTRDPEFIE KLDRLMEVTD LILLDIKQIN PEKHLKLTTK
160 170 180 190 200
SNAPIIDFAH YLRDKEQPIW IRHVLIPTKT DDPEDLTKLH EFIQTLPNVK
210 220 230 240
QVDVLPYHTM GVYKWKEMGI RYPLEGIEAP EEEVVALANK ILETSSYK
Length:248
Mass (Da):28,115
Last modified:March 15, 2005 - v1
Checksum:i0A6F5BBA95CBEBC9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL596168 Genomic DNA. Translation: CAC96675.1.
PIRiAC1613.
RefSeqiNP_470780.1. NC_003212.1.

Genome annotation databases

EnsemblBacteriaiCAC96675; CAC96675; CAC96675.
GeneIDi1130045.
KEGGilin:lin1444.
PATRICi20299621. VBILisInn102668_1477.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL596168 Genomic DNA. Translation: CAC96675.1.
PIRiAC1613.
RefSeqiNP_470780.1. NC_003212.1.

3D structure databases

ProteinModelPortaliP0A443.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272626.lin1444.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC96675; CAC96675; CAC96675.
GeneIDi1130045.
KEGGilin:lin1444.
PATRICi20299621. VBILisInn102668_1477.

Organism-specific databases

GenoListiLIN1444.

Phylogenomic databases

eggNOGiCOG1180.
HOGENOMiHOG000011458.
KOiK04069.
OMAiRFVLFMQ.
OrthoDBiEOG64FKHC.

Family and domain databases

InterProiIPR012838. PFL_activating.
IPR001989. Radical_activat_CS.
IPR007197. rSAM.
[Graphical view]
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02493. PFLA. 1 hit.
PROSITEiPS01087. RADICAL_ACTIVATING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CLIP 11262.

Entry informationi

Entry nameiPFLA_LISIN
AccessioniPrimary (citable) accession number: P0A443
Secondary accession number(s): Q9X767
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: January 7, 2015
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.