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P0A442

- PFLA_LISMO

UniProt

P0A442 - PFLA_LISMO

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Protein

Pyruvate formate-lyase-activating enzyme

Gene

pflA

Organism
Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

Activation of pyruvate formate-lyase under anaerobic conditions by generation of an organic free radical, using S-adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine.By similarity

Catalytic activityi

S-adenosyl-L-methionine + dihydroflavodoxin + [formate C-acetyltransferase]-glycine = 5'-deoxyadenosine + L-methionine + flavodoxin semiquinone + [formate C-acetyltransferase]-glycin-2-yl radical.

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi31 – 311Iron-sulfur (4Fe-4S-S-AdoMet)By similarity
Metal bindingi35 – 351Iron-sulfur (4Fe-4S-S-AdoMet)By similarity
Metal bindingi38 – 381Iron-sulfur (4Fe-4S-S-AdoMet)By similarity

GO - Molecular functioni

  1. [formate-C-acetyltransferase]-activating enzyme activity Source: UniProtKB-EC
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciLMON169963:LMO1407-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate formate-lyase-activating enzyme (EC:1.97.1.4)
Short name:
PFL-activating enzyme
Gene namesi
Name:pflA
Synonyms:pflC
Ordered Locus Names:lmo1407
OrganismiListeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Taxonomic identifieri169963 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria
ProteomesiUP000000817: Chromosome

Organism-specific databases

GenoListiLMO1407.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 248248Pyruvate formate-lyase-activating enzymePRO_0000200532Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi169963.lmo1407.

Structurei

3D structure databases

ProteinModelPortaliP0A442.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1180.
HOGENOMiHOG000011458.
KOiK04069.
OMAiWEMETNK.
OrthoDBiEOG64FKHC.
PhylomeDBiP0A442.

Family and domain databases

InterProiIPR012838. PFL_activating.
IPR001989. Radical_activat_CS.
IPR007197. rSAM.
[Graphical view]
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02493. PFLA. 1 hit.
PROSITEiPS01087. RADICAL_ACTIVATING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A442-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTEVLGRVHS VETMGTVDGP GIRFIVFMQG CLLRCQFCHN PDTWKIGTGT
60 70 80 90 100
ERSAQDVFDE AIKYKEFWDA SGGGVTVSGG EPLLQVDFLI EFFTLCKAAG
110 120 130 140 150
VHTTIDSCGG CFTRDPEFIE KLDRLMEVTD LILLDIKQIN PEKHLKLTTK
160 170 180 190 200
SNAPIIDFAH YLRDKEQPIW IRHVLIPTKT DDPEDLTKLH EFIQTLPNVK
210 220 230 240
QVDVLPYHTM GVYKWKEMGI RYPLEGIEAP EEEVVALANK ILETSSYK
Length:248
Mass (Da):28,115
Last modified:March 15, 2005 - v1
Checksum:i0A6F5BBA95CBEBC9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ009627 Genomic DNA. Translation: CAB43713.1.
AL591979 Genomic DNA. Translation: CAC99485.1.
PIRiAG1250.
T46708.
RefSeqiNP_464932.1. NC_003210.1.
YP_007603754.1. NC_020557.1.
YP_008271858.1. NC_021823.1.
YP_008275798.1. NC_021824.1.
YP_008278589.1. NC_021825.1.
YP_008279903.1. NC_021826.1.
YP_008284375.1. NC_021827.1.
YP_008296620.1. NC_021837.1.
YP_008298801.1. NC_021838.1.
YP_008301747.1. NC_021839.1.
YP_008304739.1. NC_021840.1.

Genome annotation databases

EnsemblBacteriaiCAC99485; CAC99485; CAC99485.
GeneIDi14825963.
16307197.
16311254.
16314148.
16318679.
16326319.
16333760.
16336015.
16339010.
16342218.
986485.
KEGGilmo:lmo1407.
lmoe:BN418_1653.
PATRICi20311939. VBILisMon69206_1446.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ009627 Genomic DNA. Translation: CAB43713.1 .
AL591979 Genomic DNA. Translation: CAC99485.1 .
PIRi AG1250.
T46708.
RefSeqi NP_464932.1. NC_003210.1.
YP_007603754.1. NC_020557.1.
YP_008271858.1. NC_021823.1.
YP_008275798.1. NC_021824.1.
YP_008278589.1. NC_021825.1.
YP_008279903.1. NC_021826.1.
YP_008284375.1. NC_021827.1.
YP_008296620.1. NC_021837.1.
YP_008298801.1. NC_021838.1.
YP_008301747.1. NC_021839.1.
YP_008304739.1. NC_021840.1.

3D structure databases

ProteinModelPortali P0A442.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 169963.lmo1407.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAC99485 ; CAC99485 ; CAC99485 .
GeneIDi 14825963.
16307197.
16311254.
16314148.
16318679.
16326319.
16333760.
16336015.
16339010.
16342218.
986485.
KEGGi lmo:lmo1407.
lmoe:BN418_1653.
PATRICi 20311939. VBILisMon69206_1446.

Organism-specific databases

GenoListi LMO1407.

Phylogenomic databases

eggNOGi COG1180.
HOGENOMi HOG000011458.
KOi K04069.
OMAi WEMETNK.
OrthoDBi EOG64FKHC.
PhylomeDBi P0A442.

Enzyme and pathway databases

BioCyci LMON169963:LMO1407-MONOMER.

Family and domain databases

InterProi IPR012838. PFL_activating.
IPR001989. Radical_activat_CS.
IPR007197. rSAM.
[Graphical view ]
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR02493. PFLA. 1 hit.
PROSITEi PS01087. RADICAL_ACTIVATING. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of a new locus in Listeria monocytogenes involved in cellobiose-dependent repression of hly expression."
    Huillet E.E.H., Larpin S., Pardon P., Berche P.
    FEMS Microbiol. Lett. 174:265-272(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-679 / EGD-e.

Entry informationi

Entry nameiPFLA_LISMO
AccessioniPrimary (citable) accession number: P0A442
Secondary accession number(s): Q9X767
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: November 26, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3