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Protein

Pyruvate formate-lyase-activating enzyme

Gene

pflA

Organism
Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Activation of pyruvate formate-lyase under anaerobic conditions by generation of an organic free radical, using S-adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine.By similarity

Catalytic activityi

S-adenosyl-L-methionine + dihydroflavodoxin + [formate C-acetyltransferase]-glycine = 5'-deoxyadenosine + L-methionine + flavodoxin semiquinone + [formate C-acetyltransferase]-glycin-2-yl radical.

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi31Iron-sulfur (4Fe-4S-S-AdoMet)By similarity1
Metal bindingi35Iron-sulfur (4Fe-4S-S-AdoMet)By similarity1
Metal bindingi38Iron-sulfur (4Fe-4S-S-AdoMet)By similarity1
Binding sitei80S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1
Binding sitei208S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenBy similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate formate-lyase-activating enzyme (EC:1.97.1.4)
Short name:
PFL-activating enzyme
Gene namesi
Name:pflA
Synonyms:pflC
Ordered Locus Names:lmo1407
OrganismiListeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Taxonomic identifieri169963 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria
Proteomesi
  • UP000000817 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002005321 – 248Pyruvate formate-lyase-activating enzymeAdd BLAST248

Proteomic databases

PaxDbiP0A442.

Interactioni

Protein-protein interaction databases

STRINGi169963.lmo1407.

Structurei

3D structure databases

ProteinModelPortaliP0A442.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni37 – 39S-adenosyl-L-methionine bindingBy similarity3
Regioni135 – 137S-adenosyl-L-methionine bindingBy similarity3

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105F1A. Bacteria.
COG1180. LUCA.
HOGENOMiHOG000011458.
KOiK04069.
OMAiMGVYKWE.
PhylomeDBiP0A442.

Family and domain databases

InterProiIPR012838. PFL_activating.
IPR001989. Radical_activat_CS.
IPR007197. rSAM.
[Graphical view]
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02493. PFLA. 1 hit.
PROSITEiPS01087. RADICAL_ACTIVATING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A442-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEVLGRVHS VETMGTVDGP GIRFIVFMQG CLLRCQFCHN PDTWKIGTGT
60 70 80 90 100
ERSAQDVFDE AIKYKEFWDA SGGGVTVSGG EPLLQVDFLI EFFTLCKAAG
110 120 130 140 150
VHTTIDSCGG CFTRDPEFIE KLDRLMEVTD LILLDIKQIN PEKHLKLTTK
160 170 180 190 200
SNAPIIDFAH YLRDKEQPIW IRHVLIPTKT DDPEDLTKLH EFIQTLPNVK
210 220 230 240
QVDVLPYHTM GVYKWKEMGI RYPLEGIEAP EEEVVALANK ILETSSYK
Length:248
Mass (Da):28,115
Last modified:March 15, 2005 - v1
Checksum:i0A6F5BBA95CBEBC9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ009627 Genomic DNA. Translation: CAB43713.1.
AL591979 Genomic DNA. Translation: CAC99485.1.
PIRiAG1250.
T46708.
RefSeqiNP_464932.1. NC_003210.1.
WP_003721912.1. NC_003210.1.

Genome annotation databases

EnsemblBacteriaiCAC99485; CAC99485; CAC99485.
GeneIDi986485.
KEGGilmo:lmo1407.
PATRICi20311939. VBILisMon69206_1446.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ009627 Genomic DNA. Translation: CAB43713.1.
AL591979 Genomic DNA. Translation: CAC99485.1.
PIRiAG1250.
T46708.
RefSeqiNP_464932.1. NC_003210.1.
WP_003721912.1. NC_003210.1.

3D structure databases

ProteinModelPortaliP0A442.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi169963.lmo1407.

Proteomic databases

PaxDbiP0A442.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC99485; CAC99485; CAC99485.
GeneIDi986485.
KEGGilmo:lmo1407.
PATRICi20311939. VBILisMon69206_1446.

Phylogenomic databases

eggNOGiENOG4105F1A. Bacteria.
COG1180. LUCA.
HOGENOMiHOG000011458.
KOiK04069.
OMAiMGVYKWE.
PhylomeDBiP0A442.

Family and domain databases

InterProiIPR012838. PFL_activating.
IPR001989. Radical_activat_CS.
IPR007197. rSAM.
[Graphical view]
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02493. PFLA. 1 hit.
PROSITEiPS01087. RADICAL_ACTIVATING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPFLA_LISMO
AccessioniPrimary (citable) accession number: P0A442
Secondary accession number(s): Q9X767
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: November 2, 2016
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.