Reviewed, UniProtKB/Swiss-Prot P0A437 (PTKB_SHIFL)

Names and origin

Protein names

Recommended name:
    Galactitol-specific phosphotransferase enzyme IIB component
    EC=2.7.1.69
Alternative name(s):
    EIIB-Gat
    PTS system galactitol-specific EIIB component

Gene names

Name:	gatB
Ordered Locus Names:	SF2155, S2281

Organism

Shigella flexneri [Complete proteome] [HAMAP]

Taxonomic identifier

623 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Shigella

Protein attributes

Sequence length	94 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system is involved in galactitol transport By similarity.
Catalytic activity	Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.
Subcellular location	Cytoplasm By similarity.
Domain	The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.
Sequence similarities	Contains 1 PTS EIIB type-2 domain.

Ontologies

Keywords
Biological process	Galactitol metabolism Phosphotransferase system Sugar transport Transport
Cellular component	Cytoplasm
Molecular function	Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	galactitol metabolic process Inferred from electronic annotation. Source: UniProtKB-KW phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Inferred from electronic annotation. Source: EC sugar:hydrogen symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 94	94	Galactitol-specific phosphotransferase enzyme IIB component		PRO_0000186575
Regions
	Domain	1 – 94	94	PTS EIIB type-2

Sequences

	Sequence		Length	Mass (Da)
	P0A437-1 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 44D4988E0ACACCCF	FASTA	94	10,195
	10 20 30 40 50 60 MKRKIIVACG GAVATSTMAA EEIKELCQSH NIPVELIQCR VNEIETYMDG VHLICTTARV 70 80 90 DRSFGDIPLV HGMPFVSGVG IEALQNKILT ILQG

References

[1]

"Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157." Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y. , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J. Nucleic Acids Res. 30:4432-4441(2002) [PubMed: 12384590] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 301 / Serotype 2a.

[2]

"Complete genome sequence and comparative genomics of Shigella flexneri serotype 2a strain 2457T." Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G., Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T., Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R. Infect. Immun. 71:2775-2786(2003) [PubMed: 12704152] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700930 / 2457T / Serotype 2a.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE005674 Genomic DNA. Translation: AAN43688.1. AE014073 Genomic DNA. Translation: AAP17514.1.
RefSeq	NP_707981.1. NP_837705.1.
3D structure databases
SMR	P0A437. Positions 2-94.
ModBase	Search...
Genome annotation databases
GeneID	1027334. 1078580.
GenomeReviews	Gene locus SF2155 in contig AE005674_GR. Gene locus S2281 in contig AE014073_GR.
KEGG	sfl:SF2155. sfx:S2281.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P0A437.
OMA	PFISGVG.
Enzyme and pathway databases
BioCyc	SFLE198214:AAN43688.1-MON.
BRENDA	2.7.1.69. 189495.
Family and domain databases
InterPro	IPR013011. PTS_EIIB_2. IPR003501. PTS_IIB_lac. [Graphical view]
Pfam	PF02302. PTS_IIB. 1 hit. [Graphical view]
PROSITE	PS51099. PTS_EIIB_TYPE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTKB_SHIFL

Accession

Primary (citable) accession number: P0A437
Secondary accession number(s): Q8X7H5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	November 3, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)