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P0A433

- OPD_SPHSA

UniProt

P0A433 - OPD_SPHSA

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Protein

Parathion hydrolase

Gene

opd

Organism
Sphingobium fuliginis (strain ATCC 27551)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Has an unusual substrate specificity for synthetic organophosphate triesters and phosphorofluoridates. All of the phosphate triesters found to be substrates are synthetic compounds. The identity of any naturally occurring substrate for the enzyme is unknown. Has no detectable activity with phosphate monoesters or diesters and no activity as an esterase or protease. It catalyzes the hydrolysis of the insecticide paraoxon at a rate approaching the diffusion limit and thus appears to be optimally evolved for utilizing this synthetic substrate (By similarity).By similarity

Catalytic activityi

An aryl dialkyl phosphate + H2O = dialkyl phosphate + an aryl alcohol.

Cofactori

Binds 2 zinc ions per subunit.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi55 – 551Zinc 1
Metal bindingi57 – 571Zinc 1
Metal bindingi169 – 1691Zinc 1; via carbamate group
Metal bindingi169 – 1691Zinc 2; via carbamate group
Metal bindingi201 – 2011Zinc 2
Metal bindingi230 – 2301Zinc 2
Metal bindingi301 – 3011Zinc 1

GO - Molecular functioni

  1. aryldialkylphosphatase activity Source: UniProtKB-EC
  2. zinc ion binding Source: InterPro

GO - Biological processi

  1. catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-3321.

Names & Taxonomyi

Protein namesi
Recommended name:
Parathion hydrolase (EC:3.1.8.1)
Alternative name(s):
Phosphotriesterase
Short name:
PTE
Gene namesi
Name:opd
Encoded oniPlasmid pPDL20 Publication
OrganismiSphingobium fuliginis (strain ATCC 27551)
Taxonomic identifieri336203 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeSphingobium

Subcellular locationi

Cell membrane By similarity; Peripheral membrane protein By similarity

GO - Cellular componenti

  1. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929Tat-type signal1 PublicationPROSITE-ProRule annotationAdd
BLAST
Chaini30 – 365336Parathion hydrolasePRO_0000029861Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei169 – 1691N6-carboxylysine1 PublicationPROSITE-ProRule annotation

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has been experimentally proven.

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

Secondary structure

1
365
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi36 – 394
Beta strandi42 – 454
Helixi46 – 483
Beta strandi51 – 566
Beta strandi58 – 603
Helixi65 – 684
Helixi70 – 734
Helixi76 – 9217
Beta strandi97 – 1004
Helixi104 – 1063
Helixi110 – 12011
Beta strandi123 – 1253
Beta strandi127 – 1293
Helixi136 – 1394
Helixi143 – 15513
Beta strandi167 – 1715
Beta strandi173 – 1753
Helixi178 – 19417
Beta strandi198 – 2014
Helixi204 – 2063
Helixi208 – 21811
Helixi223 – 2253
Beta strandi226 – 2283
Helixi231 – 2333
Helixi237 – 2459
Beta strandi249 – 2524
Helixi266 – 2727
Helixi277 – 28913
Helixi293 – 2953
Beta strandi296 – 2983
Beta strandi304 – 3063
Beta strandi308 – 3103
Helixi313 – 3208
Helixi324 – 3263
Helixi327 – 33711
Turni338 – 3403
Helixi343 – 3508
Helixi352 – 3587

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1P6BX-ray1.90A/B30-365[»]
1P6CX-ray2.00A/B30-365[»]
ProteinModelPortaliP0A433.
SMRiP0A433. Positions 34-364.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A433.

Family & Domainsi

Sequence similaritiesi

Belongs to the phosphotriesterase family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Family and domain databases

InterProiIPR017947. AryldialkylPase_Zn-BS.
IPR001559. Aryldialkylphosphatase.
IPR006311. TAT_signal.
[Graphical view]
PANTHERiPTHR10819. PTHR10819. 1 hit.
PfamiPF02126. PTE. 1 hit.
[Graphical view]
PROSITEiPS01322. PHOSPHOTRIESTERASE_1. 1 hit.
PS51347. PHOSPHOTRIESTERASE_2. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A433-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQTRRVVLKS AAAAGTLLGG LAGCASVAGS IGTGDRINTV RGPITISEAG
60 70 80 90 100
FTLTHEHICG SSAGFLRAWP EFFGSRKALA EKAVRGLRRA RAAGVRTIVD
110 120 130 140 150
VSTFDIGRDV SLLAEVSRAA DVHIVAATGL WFDPPLSMRL RSVEELTQFF
160 170 180 190 200
LREIQYGIED TGIRAGIIKV ATTGKATPFQ ELVLKAAARA SLATGVPVTT
210 220 230 240 250
HTAASQRDGE QQAAIFESEG LSPSRVCIGH SDDTDDLSYL TALAARGYLI
260 270 280 290 300
GLDHIPHSAI GLEDNASASA LLGIRSWQTR ALLIKALIDQ GYMKQILVSN
310 320 330 340 350
DWLFGFSSYV TNIMDVMDRV NPDGMAFIPL RVIPFLREKG VPQETLAGIT
360
VTNPARFLSP TLRAS
Length:365
Mass (Da):39,004
Last modified:March 15, 2005 - v1
Checksum:i41FF8E4B029B46DC
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M29593 Genomic DNA. Translation: AAA24930.1.
PIRiA43720.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M29593 Genomic DNA. Translation: AAA24930.1 .
PIRi A43720.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1P6B X-ray 1.90 A/B 30-365 [» ]
1P6C X-ray 2.00 A/B 30-365 [» ]
ProteinModelPortali P0A433.
SMRi P0A433. Positions 34-364.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-3321.

Miscellaneous databases

EvolutionaryTracei P0A433.

Family and domain databases

InterProi IPR017947. AryldialkylPase_Zn-BS.
IPR001559. Aryldialkylphosphatase.
IPR006311. TAT_signal.
[Graphical view ]
PANTHERi PTHR10819. PTHR10819. 1 hit.
Pfami PF02126. PTE. 1 hit.
[Graphical view ]
PROSITEi PS01322. PHOSPHOTRIESTERASE_1. 1 hit.
PS51347. PHOSPHOTRIESTERASE_2. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Parathion hydrolase specified by the Flavobacterium opd gene: relationship between the gene and protein."
    Mulbry W.W., Karns J.S.
    J. Bacteriol. 171:6740-6746(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 30-53.
  2. "Enhanced degradation of chemical warfare agents through molecular engineering of the phosphotriesterase active site."
    Hill C.M., Li W.S., Thoden J.B., Holden H.M., Raushel F.M.
    J. Am. Chem. Soc. 125:8990-8991(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 30-365, CARBAMYLATION AT LYS-169.

Entry informationi

Entry nameiOPD_SPHSA
AccessioniPrimary (citable) accession number: P0A433
Secondary accession number(s): P13739, P16648
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: October 29, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3