P0A415 (PSAC_THEEB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Photosystem I iron-sulfur center
EC=1.97.1.12

Alternative name(s):
9 kDa polypeptide
PSI-C
Photosystem I subunit VII
PsaC

Gene names

Name:	psaC
Ordered Locus Names:	tsl1013

Organism

Thermosynechococcus elongatus (strain BP-1) [Reference proteome] [HAMAP]

Taxonomic identifier

197221 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Cyanobacteria › Oscillatoriophycideae › Chroococcales › Thermosynechococcus › Thermosynechococcus elongatus

Protein attributes

Sequence length	81 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Apoprotein for the two 4Fe-4S centers FA and FB of photosystem I (PSI); essential for photochemical activity. FB is the terminal electron acceptor of PSI, donating electrons to ferredoxin. The C-terminus interacts with PsaA/B/D and helps assemble the protein into the PSI complex. Required for binding of PsaD and PsaE to PSI. PSI is a plastocyanin/cytochrome c6-ferredoxin oxidoreductase, converting photonic excitation into a charge separation, which transfers an electron from the donor P700 chlorophyll pair to the spectroscopically characterized acceptors A0, A1, FX, FA and FB in turn. HAMAP-Rule MF_01303
Catalytic activity	Reduced plastocyanin + oxidized ferredoxin + light = oxidized plastocyanin + reduced ferredoxin. HAMAP-Rule MF_01303
Cofactor	Binds 2 4Fe-4S clusters. Cluster 2 is most probably the spectroscopically characterized electron acceptor FA and cluster 1 is most probably FB By similarity.
Subunit structure	The cyanobacterial PSI reaction center is composed of one copy each of PsaA,B,C,D,E,F,I,J,K,L,M and X, and forms trimeric complexes By similarity.
Subcellular location	Cellular thylakoid membrane; Peripheral membrane protein; Cytoplasmic side By similarity HAMAP-Rule MF_01303.
Sequence similarities	Contains 2 4Fe-4S ferredoxin-type domains.

Ontologies

Keywords
Biological process	Electron transport Photosynthesis Transport
Cellular component	Membrane Photosystem I Thylakoid
Domain	Repeat
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	photosynthetic electron transport in photosystem I Inferred from electronic annotation. Source: InterPro
Cellular_component	photosystem I Inferred from electronic annotation. Source: UniProtKB-KW thylakoid membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed By similarity

Chain

2 – 81

80

Photosystem I iron-sulfur center HAMAP-Rule MF_01303

PRO_0000062018

Regions

Domain

2 – 31

30

4Fe-4S ferredoxin-type 1

Domain

37 – 68

32

4Fe-4S ferredoxin-type 2

Sites

Metal binding

11

1

Iron-sulfur 1 (4Fe-4S)

Metal binding

14

1

Iron-sulfur 1 (4Fe-4S)

Metal binding

17

1

Iron-sulfur 1 (4Fe-4S)

Metal binding

21

1

Iron-sulfur 2 (4Fe-4S)

Metal binding

48

1

Iron-sulfur 2 (4Fe-4S)

Metal binding

51

1

Iron-sulfur 2 (4Fe-4S)

Metal binding

54

1

Iron-sulfur 2 (4Fe-4S)

Metal binding

58

1

Iron-sulfur 1 (4Fe-4S)

Secondary structure

1

.

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81

Helix Strand Turn

Details...

Beta strand

4 – 8

5

Helix

16 – 20

5

Beta strand

27 – 30

4

Beta strand

32 – 34

3

Beta strand

37 – 41

5

Helix

45 – 47

3

Helix

53 – 57

5

Beta strand

60 – 62

3

Beta strand

64 – 68

5

Turn

74 – 78

5

Sequences

	Sequence		Length	Mass (Da)
	P0A415 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: ED5362F4D6E48F36	FASTA	81	8,800
	10 20 30 40 50 60 MAHTVKIYDT CIGCTQCVRA CPTDVLEMVP WDGCKAGQIA SSPRTEDCVG CKRCETACPT 70 80 DFLSIRVYLG AETTRSMGLA Y

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome structure of the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1." Nakamura Y., Kaneko T., Sato S., Ikeuchi M., Katoh H., Sasamoto S., Watanabe A., Iriguchi M., Kawashima K., Kimura T., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Nakazaki N., Shimpo S., Sugimoto M. , Takeuchi C., Yamada M., Tabata S. DNA Res. 9:123-130(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BP-1.
[2]	"Structure of photosystem I." Fromme P., Jordan P., Krauss N. Biochim. Biophys. Acta 1507:5-31(2001) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.
[3]	"Photosystem I at 4-A resolution represents the first structural model of a joint photosynthetic reaction centre and core antenna system." Krauss N., Schubert W.-D., Klukas O., Fromme P., Witt H.T., Saenger W. Nat. Struct. Biol. 3:965-973(1996) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (4.0 ANGSTROMS).
[4]	"Photosystem I, an improved model of the stromal subunits PsaC, PsaD, and PsaE." Klukas O., Schubert W.-D., Jordan P., Krauss N., Fromme P., Witt H.T., Saenger W. J. Biol. Chem. 274:7351-7360(1999) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (4.0 ANGSTROMS).
[5]	"Three-dimensional structure of cyanobacterial photosystem I at 2.5 A resolution." Jordan P., Fromme P., Witt H.T., Klukas O., Saenger W., Krauss N. Nature 411:909-917(2001) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

BA000039 Genomic DNA. Translation: BAC08565.1.

RefSeq

NP_681803.1. NC_004113.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1C51	X-ray	4.0
1JB0	X-ray	2.50	C	2-81	[»]
2PPS	X-ray	4.0
3PCQ	X-ray	8.98	C	2-81	[»]
4FE1	X-ray	4.92	C	2-81	[»]

ProteinModelPortal

P0A415.

SMR

P0A415. Positions 2-81.

ModBase

Search...

Protein-protein interaction databases

STRING

197221.tsl1013.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

BAC08565; BAC08565; BAC08565.

GeneID

1011306.

KEGG

tel:tsl1013.

PATRIC

23927326. VBITheElo119873_1063.

Phylogenomic databases

eggNOG

COG1145.

HOGENOM

HOG000230505.

KO

K02691.

OMA

EDCIGCK.

ProtClustDB

PRK02651.

Family and domain databases

HAMAP

MF_01303. PSI_PsaC.

InterPro

IPR001450. 4Fe4S-bd_dom.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR017491. PSI_PsaC.
[Graphical view]

Pfam

PF12838. Fer4_7. 1 hit.
[Graphical view]

TIGRFAMs

TIGR03048. PS_I_psaC. 1 hit.

PROSITE

PS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P0A415.

Entry information

Entry name

PSAC_THEEB

Accession

Primary (citable) accession number: P0A415
Secondary accession number(s): P18083, P20451

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 68 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program