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Protein

Aminoglycoside 3'-phosphotransferase

Gene

aphA

Organism
Enterococcus faecalis (Streptococcus faecalis)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Resistance to kanamycin and structurally-related aminoglycosides, including amikacin.

Catalytic activityi

ATP + kanamycin = ADP + kanamycin 3'-phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei190Proton acceptorBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Antibiotic resistance

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

SABIO-RKP0A3Y5.

Names & Taxonomyi

Protein namesi
Recommended name:
Aminoglycoside 3'-phosphotransferase (EC:2.7.1.95)
Alternative name(s):
APH(3')III
Kanamycin kinase, type III
Neomycin-kanamycin phosphotransferase type III
Gene namesi
Name:aphA
Encoded oniPlasmid pJH10 Publication
OrganismiEnterococcus faecalis (Streptococcus faecalis)
Taxonomic identifieri1351 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesEnterococcaceaeEnterococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002048051 – 264Aminoglycoside 3'-phosphotransferaseAdd BLAST264

Proteomic databases

PRIDEiP0A3Y5.

Structurei

Secondary structure

1264
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi8 – 14Combined sources7
Beta strandi17 – 21Combined sources5
Beta strandi27 – 35Combined sources9
Beta strandi40 – 46Combined sources7
Helixi48 – 50Combined sources3
Helixi57 – 67Combined sources11
Turni68 – 70Combined sources3
Beta strandi76 – 82Combined sources7
Beta strandi85 – 91Combined sources7
Beta strandi94 – 97Combined sources4
Helixi98 – 101Combined sources4
Turni104 – 106Combined sources3
Helixi108 – 124Combined sources17
Helixi136 – 148Combined sources13
Helixi156 – 159Combined sources4
Beta strandi164 – 167Combined sources4
Helixi168 – 177Combined sources10
Beta strandi184 – 187Combined sources4
Beta strandi195 – 199Combined sources5
Beta strandi202 – 206Combined sources5
Beta strandi213 – 216Combined sources4
Helixi218 – 231Combined sources14
Helixi236 – 245Combined sources10
Helixi251 – 260Combined sources10
Helixi261 – 263Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1J7IX-ray3.20A1-264[»]
1J7LX-ray2.20A/B1-264[»]
1J7UX-ray2.40A/B1-264[»]
1L8TX-ray2.40A2-264[»]
2B0QX-ray2.70A2-264[»]
2BKKX-ray2.15A/C1-264[»]
3Q2JX-ray2.15A/B1-264[»]
3TM0X-ray2.10A2-264[»]
ProteinModelPortaliP0A3Y5.
SMRiP0A3Y5.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A3Y5.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

KOiK19299.

Family and domain databases

CDDicd05150. APH. 1 hit.
InterProiIPR002575. Aminoglycoside_PTrfase.
IPR024165. Kan/Strep_kinase.
IPR011009. Kinase-like_dom.
[Graphical view]
PfamiPF01636. APH. 1 hit.
[Graphical view]
PIRSFiPIRSF000706. Kanamycin_kin. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.

Sequencei

Sequence statusi: Complete.

P0A3Y5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKMRISPEL KKLIEKYRCV KDTEGMSPAK VYKLVGENEN LYLKMTDSRY
60 70 80 90 100
KGTTYDVERE KDMMLWLEGK LPVPKVLHFE RHDGWSNLLM SEADGVLCSE
110 120 130 140 150
EYEDEQSPEK IIELYAECIR LFHSIDISDC PYTNSLDSRL AELDYLLNND
160 170 180 190 200
LADVDCENWE EDTPFKDPRE LYDFLKTEKP EEELVFSHGD LGDSNIFVKD
210 220 230 240 250
GKVSGFIDLG RSGRADKWYD IAFCVRSIRE DIGEEQYVEL FFDLLGIKPD
260
WEKIKYYILL DELF
Length:264
Mass (Da):30,974
Last modified:July 21, 1986 - v1
Checksum:i7EEA5851D3380C5A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01547 Genomic DNA. Translation: CAA24789.1.
X99398 Genomic DNA. Translation: CAA67773.1.
PIRiA00665. PKSOJF.
RefSeqiWP_001096887.1. NZ_MBRC01000045.1.
YP_783930.1. NC_008445.1.

Genome annotation databases

GeneIDi23533821.
4362702.
KEGGiag:CAA24789.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01547 Genomic DNA. Translation: CAA24789.1.
X99398 Genomic DNA. Translation: CAA67773.1.
PIRiA00665. PKSOJF.
RefSeqiWP_001096887.1. NZ_MBRC01000045.1.
YP_783930.1. NC_008445.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1J7IX-ray3.20A1-264[»]
1J7LX-ray2.20A/B1-264[»]
1J7UX-ray2.40A/B1-264[»]
1L8TX-ray2.40A2-264[»]
2B0QX-ray2.70A2-264[»]
2BKKX-ray2.15A/C1-264[»]
3Q2JX-ray2.15A/B1-264[»]
3TM0X-ray2.10A2-264[»]
ProteinModelPortaliP0A3Y5.
SMRiP0A3Y5.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP0A3Y5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi23533821.
4362702.
KEGGiag:CAA24789.

Phylogenomic databases

KOiK19299.

Enzyme and pathway databases

SABIO-RKP0A3Y5.

Miscellaneous databases

EvolutionaryTraceiP0A3Y5.
PROiP0A3Y5.

Family and domain databases

CDDicd05150. APH. 1 hit.
InterProiIPR002575. Aminoglycoside_PTrfase.
IPR024165. Kan/Strep_kinase.
IPR011009. Kinase-like_dom.
[Graphical view]
PfamiPF01636. APH. 1 hit.
[Graphical view]
PIRSFiPIRSF000706. Kanamycin_kin. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiKKA3_ENTFL
AccessioniPrimary (citable) accession number: P0A3Y5
Secondary accession number(s): P00554
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 30, 2016
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.