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Protein

Aminoglycoside 3'-phosphotransferase

Gene

aphA

Organism
Enterococcus faecalis (Streptococcus faecalis)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Resistance to kanamycin and structurally-related aminoglycosides, including amikacin.

Catalytic activityi

ATP + kanamycin = ADP + kanamycin 3'-phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei190Proton acceptorBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processAntibiotic resistance
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

SABIO-RKP0A3Y5

Names & Taxonomyi

Protein namesi
Recommended name:
Aminoglycoside 3'-phosphotransferase (EC:2.7.1.95)
Alternative name(s):
APH(3')III
Kanamycin kinase, type III
Neomycin-kanamycin phosphotransferase type III
Gene namesi
Name:aphA
Encoded oniPlasmid pJH10 Publication
OrganismiEnterococcus faecalis (Streptococcus faecalis)
Taxonomic identifieri1351 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesEnterococcaceaeEnterococcus

Pathology & Biotechi

Chemistry databases

DrugBankiDB04395 Phosphoaminophosphonic Acid-Adenylate Ester

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002048051 – 264Aminoglycoside 3'-phosphotransferaseAdd BLAST264

Proteomic databases

PRIDEiP0A3Y5

Structurei

Secondary structure

1264
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi8 – 14Combined sources7
Beta strandi17 – 21Combined sources5
Beta strandi27 – 35Combined sources9
Beta strandi40 – 46Combined sources7
Helixi48 – 50Combined sources3
Helixi57 – 67Combined sources11
Turni68 – 70Combined sources3
Beta strandi76 – 82Combined sources7
Beta strandi85 – 91Combined sources7
Beta strandi94 – 97Combined sources4
Helixi98 – 101Combined sources4
Turni104 – 106Combined sources3
Helixi108 – 124Combined sources17
Helixi136 – 148Combined sources13
Helixi156 – 159Combined sources4
Beta strandi164 – 167Combined sources4
Helixi168 – 177Combined sources10
Beta strandi184 – 187Combined sources4
Beta strandi195 – 199Combined sources5
Beta strandi202 – 206Combined sources5
Beta strandi213 – 216Combined sources4
Helixi218 – 231Combined sources14
Helixi236 – 245Combined sources10
Helixi251 – 260Combined sources10
Helixi261 – 263Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1J7IX-ray3.20A1-264[»]
1J7LX-ray2.20A/B1-264[»]
1J7UX-ray2.40A/B1-264[»]
1L8TX-ray2.40A2-264[»]
2B0QX-ray2.70A2-264[»]
2BKKX-ray2.15A/C1-264[»]
3Q2JX-ray2.15A/B1-264[»]
3TM0X-ray2.10A2-264[»]
ProteinModelPortaliP0A3Y5
SMRiP0A3Y5
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A3Y5

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

KOiK19299

Family and domain databases

CDDicd05150 APH, 1 hit
InterProiView protein in InterPro
IPR002575 Aminoglycoside_PTrfase
IPR024165 Kan/Strep_kinase
IPR011009 Kinase-like_dom_sf
PfamiView protein in Pfam
PF01636 APH, 1 hit
PIRSFiPIRSF000706 Kanamycin_kin, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit

Sequencei

Sequence statusi: Complete.

P0A3Y5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKMRISPEL KKLIEKYRCV KDTEGMSPAK VYKLVGENEN LYLKMTDSRY
60 70 80 90 100
KGTTYDVERE KDMMLWLEGK LPVPKVLHFE RHDGWSNLLM SEADGVLCSE
110 120 130 140 150
EYEDEQSPEK IIELYAECIR LFHSIDISDC PYTNSLDSRL AELDYLLNND
160 170 180 190 200
LADVDCENWE EDTPFKDPRE LYDFLKTEKP EEELVFSHGD LGDSNIFVKD
210 220 230 240 250
GKVSGFIDLG RSGRADKWYD IAFCVRSIRE DIGEEQYVEL FFDLLGIKPD
260
WEKIKYYILL DELF
Length:264
Mass (Da):30,974
Last modified:July 21, 1986 - v1
Checksum:i7EEA5851D3380C5A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01547 Genomic DNA Translation: CAA24789.1
X99398 Genomic DNA Translation: CAA67773.1
PIRiA00665 PKSOJF
RefSeqiWP_001096887.1, NZ_PUBU01000092.1
YP_783930.1, NC_008445.1

Genome annotation databases

GeneIDi36311921
4362702
KEGGiag:CAA24789

Entry informationi

Entry nameiKKA3_ENTFL
AccessioniPrimary (citable) accession number: P0A3Y5
Secondary accession number(s): P00554
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: May 23, 2018
This is version 72 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Plasmid
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Main funding by: National Institutes of Health