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P0A3Y5

- KKA3_ENTFL

UniProt

P0A3Y5 - KKA3_ENTFL

Protein

Aminoglycoside 3'-phosphotransferase

Gene

aphA

Organism
Enterococcus faecalis (Streptococcus faecalis)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 50 (01 Oct 2014)
      Sequence version 1 (21 Jul 1986)
      Previous versions | rss
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    Functioni

    Resistance to kanamycin and structurally-related aminoglycosides, including amikacin.

    Catalytic activityi

    ATP + kanamycin = ADP + kanamycin 3'-phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei190 – 1901Proton acceptorBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. kanamycin kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. response to antibiotic Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Antibiotic resistance

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    SABIO-RKP0A3Y5.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aminoglycoside 3'-phosphotransferase (EC:2.7.1.95)
    Alternative name(s):
    APH(3')III
    Kanamycin kinase, type III
    Neomycin-kanamycin phosphotransferase type III
    Gene namesi
    Name:aphA
    Encoded oniPlasmid pJH10 Publication
    OrganismiEnterococcus faecalis (Streptococcus faecalis)
    Taxonomic identifieri1351 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesEnterococcaceaeEnterococcus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 264264Aminoglycoside 3'-phosphotransferasePRO_0000204805Add
    BLAST

    Structurei

    Secondary structure

    1
    264
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi8 – 147
    Beta strandi17 – 215
    Beta strandi27 – 359
    Beta strandi40 – 467
    Helixi48 – 503
    Helixi57 – 6711
    Turni68 – 703
    Beta strandi76 – 827
    Beta strandi85 – 917
    Beta strandi94 – 974
    Helixi98 – 1014
    Turni104 – 1063
    Helixi108 – 12417
    Helixi136 – 14813
    Helixi156 – 1594
    Beta strandi164 – 1674
    Helixi168 – 17710
    Beta strandi184 – 1874
    Beta strandi195 – 1995
    Beta strandi202 – 2065
    Beta strandi213 – 2164
    Helixi218 – 23114
    Helixi236 – 24510
    Helixi251 – 26010
    Helixi261 – 2633

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1J7IX-ray3.20A1-264[»]
    1J7LX-ray2.20A/B1-264[»]
    1J7UX-ray2.40A/B1-264[»]
    1L8TX-ray2.40A2-264[»]
    2B0QX-ray2.70A2-264[»]
    2BKKX-ray2.15A/C1-264[»]
    3Q2JX-ray2.15A/B1-264[»]
    3TM0X-ray2.10A2-264[»]
    ProteinModelPortaliP0A3Y5.
    SMRiP0A3Y5. Positions 2-264.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0A3Y5.

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    InterProiIPR002575. Aminoglycoside_PTrfase.
    IPR024165. Kan/Strep_kinase.
    IPR011009. Kinase-like_dom.
    [Graphical view]
    PANTHERiPTHR21310. PTHR21310. 1 hit.
    PfamiPF01636. APH. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000706. Kanamycin_kin. 1 hit.
    SUPFAMiSSF56112. SSF56112. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P0A3Y5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAKMRISPEL KKLIEKYRCV KDTEGMSPAK VYKLVGENEN LYLKMTDSRY    50
    KGTTYDVERE KDMMLWLEGK LPVPKVLHFE RHDGWSNLLM SEADGVLCSE 100
    EYEDEQSPEK IIELYAECIR LFHSIDISDC PYTNSLDSRL AELDYLLNND 150
    LADVDCENWE EDTPFKDPRE LYDFLKTEKP EEELVFSHGD LGDSNIFVKD 200
    GKVSGFIDLG RSGRADKWYD IAFCVRSIRE DIGEEQYVEL FFDLLGIKPD 250
    WEKIKYYILL DELF 264
    Length:264
    Mass (Da):30,974
    Last modified:July 21, 1986 - v1
    Checksum:i7EEA5851D3380C5A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    V01547 Genomic DNA. Translation: CAA24789.1.
    X99398 Genomic DNA. Translation: CAA67773.1.
    PIRiA00665. PKSOJF.
    RefSeqiYP_783930.1. NC_008445.1.

    Genome annotation databases

    GeneIDi4362702.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    V01547 Genomic DNA. Translation: CAA24789.1 .
    X99398 Genomic DNA. Translation: CAA67773.1 .
    PIRi A00665. PKSOJF.
    RefSeqi YP_783930.1. NC_008445.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1J7I X-ray 3.20 A 1-264 [» ]
    1J7L X-ray 2.20 A/B 1-264 [» ]
    1J7U X-ray 2.40 A/B 1-264 [» ]
    1L8T X-ray 2.40 A 2-264 [» ]
    2B0Q X-ray 2.70 A 2-264 [» ]
    2BKK X-ray 2.15 A/C 1-264 [» ]
    3Q2J X-ray 2.15 A/B 1-264 [» ]
    3TM0 X-ray 2.10 A 2-264 [» ]
    ProteinModelPortali P0A3Y5.
    SMRi P0A3Y5. Positions 2-264.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 4362702.

    Enzyme and pathway databases

    SABIO-RK P0A3Y5.

    Miscellaneous databases

    EvolutionaryTracei P0A3Y5.

    Family and domain databases

    InterProi IPR002575. Aminoglycoside_PTrfase.
    IPR024165. Kan/Strep_kinase.
    IPR011009. Kinase-like_dom.
    [Graphical view ]
    PANTHERi PTHR21310. PTHR21310. 1 hit.
    Pfami PF01636. APH. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000706. Kanamycin_kin. 1 hit.
    SUPFAMi SSF56112. SSF56112. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the Streptococcus faecalis plasmid gene encoding the 3'5'-aminoglycoside phosphotransferase type III."
      Trieu-Cuot P., Courvalin P.
      Gene 23:331-341(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiKKA3_ENTFL
    AccessioniPrimary (citable) accession number: P0A3Y5
    Secondary accession number(s): P00554
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 50 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Plasmid

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3