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Protein

Aminoglycoside 3'-phosphotransferase

Gene

aphA

Organism
Enterococcus faecalis (Streptococcus faecalis)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Resistance to kanamycin and structurally-related aminoglycosides, including amikacin.

Catalytic activityi

ATP + kanamycin = ADP + kanamycin 3'-phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei190 – 1901Proton acceptorBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. kanamycin kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. response to antibiotic Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Antibiotic resistance

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

SABIO-RKP0A3Y5.

Names & Taxonomyi

Protein namesi
Recommended name:
Aminoglycoside 3'-phosphotransferase (EC:2.7.1.95)
Alternative name(s):
APH(3')III
Kanamycin kinase, type III
Neomycin-kanamycin phosphotransferase type III
Gene namesi
Name:aphA
Encoded oniPlasmid pJH10 Publication
OrganismiEnterococcus faecalis (Streptococcus faecalis)
Taxonomic identifieri1351 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesEnterococcaceaeEnterococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 264264Aminoglycoside 3'-phosphotransferasePRO_0000204805Add
BLAST

Structurei

Secondary structure

1
264
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi8 – 147Combined sources
Beta strandi17 – 215Combined sources
Beta strandi27 – 359Combined sources
Beta strandi40 – 467Combined sources
Helixi48 – 503Combined sources
Helixi57 – 6711Combined sources
Turni68 – 703Combined sources
Beta strandi76 – 827Combined sources
Beta strandi85 – 917Combined sources
Beta strandi94 – 974Combined sources
Helixi98 – 1014Combined sources
Turni104 – 1063Combined sources
Helixi108 – 12417Combined sources
Helixi136 – 14813Combined sources
Helixi156 – 1594Combined sources
Beta strandi164 – 1674Combined sources
Helixi168 – 17710Combined sources
Beta strandi184 – 1874Combined sources
Beta strandi195 – 1995Combined sources
Beta strandi202 – 2065Combined sources
Beta strandi213 – 2164Combined sources
Helixi218 – 23114Combined sources
Helixi236 – 24510Combined sources
Helixi251 – 26010Combined sources
Helixi261 – 2633Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1J7IX-ray3.20A1-264[»]
1J7LX-ray2.20A/B1-264[»]
1J7UX-ray2.40A/B1-264[»]
1L8TX-ray2.40A2-264[»]
2B0QX-ray2.70A2-264[»]
2BKKX-ray2.15A/C1-264[»]
3Q2JX-ray2.15A/B1-264[»]
3TM0X-ray2.10A2-264[»]
ProteinModelPortaliP0A3Y5.
SMRiP0A3Y5. Positions 2-264.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A3Y5.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

InterProiIPR002575. Aminoglycoside_PTrfase.
IPR024165. Kan/Strep_kinase.
IPR011009. Kinase-like_dom.
[Graphical view]
PANTHERiPTHR21310. PTHR21310. 1 hit.
PfamiPF01636. APH. 1 hit.
[Graphical view]
PIRSFiPIRSF000706. Kanamycin_kin. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.

Sequencei

Sequence statusi: Complete.

P0A3Y5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKMRISPEL KKLIEKYRCV KDTEGMSPAK VYKLVGENEN LYLKMTDSRY
60 70 80 90 100
KGTTYDVERE KDMMLWLEGK LPVPKVLHFE RHDGWSNLLM SEADGVLCSE
110 120 130 140 150
EYEDEQSPEK IIELYAECIR LFHSIDISDC PYTNSLDSRL AELDYLLNND
160 170 180 190 200
LADVDCENWE EDTPFKDPRE LYDFLKTEKP EEELVFSHGD LGDSNIFVKD
210 220 230 240 250
GKVSGFIDLG RSGRADKWYD IAFCVRSIRE DIGEEQYVEL FFDLLGIKPD
260
WEKIKYYILL DELF
Length:264
Mass (Da):30,974
Last modified:July 21, 1986 - v1
Checksum:i7EEA5851D3380C5A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01547 Genomic DNA. Translation: CAA24789.1.
X99398 Genomic DNA. Translation: CAA67773.1.
PIRiA00665. PKSOJF.
RefSeqiYP_009088946.1. NG_041215.1.
YP_783930.1. NC_008445.1.

Genome annotation databases

GeneIDi4362702.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01547 Genomic DNA. Translation: CAA24789.1.
X99398 Genomic DNA. Translation: CAA67773.1.
PIRiA00665. PKSOJF.
RefSeqiYP_009088946.1. NG_041215.1.
YP_783930.1. NC_008445.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1J7IX-ray3.20A1-264[»]
1J7LX-ray2.20A/B1-264[»]
1J7UX-ray2.40A/B1-264[»]
1L8TX-ray2.40A2-264[»]
2B0QX-ray2.70A2-264[»]
2BKKX-ray2.15A/C1-264[»]
3Q2JX-ray2.15A/B1-264[»]
3TM0X-ray2.10A2-264[»]
ProteinModelPortaliP0A3Y5.
SMRiP0A3Y5. Positions 2-264.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi4362702.

Enzyme and pathway databases

SABIO-RKP0A3Y5.

Miscellaneous databases

EvolutionaryTraceiP0A3Y5.

Family and domain databases

InterProiIPR002575. Aminoglycoside_PTrfase.
IPR024165. Kan/Strep_kinase.
IPR011009. Kinase-like_dom.
[Graphical view]
PANTHERiPTHR21310. PTHR21310. 1 hit.
PfamiPF01636. APH. 1 hit.
[Graphical view]
PIRSFiPIRSF000706. Kanamycin_kin. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Nucleotide sequence of the Streptococcus faecalis plasmid gene encoding the 3'5'-aminoglycoside phosphotransferase type III."
    Trieu-Cuot P., Courvalin P.
    Gene 23:331-341(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiKKA3_ENTFL
AccessioniPrimary (citable) accession number: P0A3Y5
Secondary accession number(s): P00554
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: January 7, 2015
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.