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P0A3Y5 (KKA3_ENTFL) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Aminoglycoside 3'-phosphotransferase
EC=2.7.1.95
Alternative name(s):
APH(3')III
Kanamycin kinase, type III
Neomycin-kanamycin phosphotransferase type III
Gene names
Name:aphA
Encoded onPlasmid pJH1
OrganismEnterococcus faecalis (Streptococcus faecalis)
Taxonomic identifier1351 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacilli › Lactobacillales › Enterococcaceae › Enterococcus

Protein attributes

Sequence length264 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Resistance to kanamycin and structurally-related aminoglycosides, including amikacin.

Catalytic activity

ATP + kanamycin = ADP + kanamycin 3'-phosphate.

Sequence similarities

Belongs to the aminoglycoside phosphotransferase family.

Ontologies

Keywords
   Biological processAntibiotic resistance
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical term3D-structure
Plasmid
Gene Ontology (GO)
   Biological_processresponse to antibiotic

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

kanamycin kinase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 264264Aminoglycoside 3'-phosphotransferase
PRO_0000204805

Sites

Active site1901Proton acceptor By similarity

Secondary structure

1 ............................................... 264
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P0A3Y5 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: 7EEA5851D3380C5A

FASTA26430,974
        10         20         30         40         50         60 
MAKMRISPEL KKLIEKYRCV KDTEGMSPAK VYKLVGENEN LYLKMTDSRY KGTTYDVERE 

        70         80         90        100        110        120 
KDMMLWLEGK LPVPKVLHFE RHDGWSNLLM SEADGVLCSE EYEDEQSPEK IIELYAECIR 

       130        140        150        160        170        180 
LFHSIDISDC PYTNSLDSRL AELDYLLNND LADVDCENWE EDTPFKDPRE LYDFLKTEKP 

       190        200        210        220        230        240 
EEELVFSHGD LGDSNIFVKD GKVSGFIDLG RSGRADKWYD IAFCVRSIRE DIGEEQYVEL 

       250        260 
FFDLLGIKPD WEKIKYYILL DELF

« Hide

References

[1]"Nucleotide sequence of the Streptococcus faecalis plasmid gene encoding the 3'5'-aminoglycoside phosphotransferase type III."
Trieu-Cuot P., Courvalin P.
Gene 23:331-341(1983) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		V01547 Genomic DNA. Translation: CAA24789.1.
X99398 Genomic DNA. Translation: CAA67773.1.
PIRPKSOJF. A00665.
RefSeqYP_783930.1. NC_008445.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1J7IX-ray3.20A1-264[»]
1J7LX-ray2.20A/B1-264[»]
1J7UX-ray2.40A/B1-264[»]
1L8TX-ray2.40A2-264[»]
2B0QX-ray2.70A2-264[»]
2BKKX-ray2.15A/C1-264[»]
3Q2JX-ray2.15A/B1-264[»]
3TM0X-ray2.10A2-264[»]
ProteinModelPortalP0A3Y5.
SMRP0A3Y5. Positions 2-264.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4362702.

Phylogenomic databases

ProtClustDBCLSK2516714.

Enzyme and pathway databases

SABIO-RKP0A3Y5.

Family and domain databases

InterProIPR002575. Aminoglycoside_PTrfase.
IPR024165. Kan/Strep_kinase.
IPR011009. Kinase-like_dom.
[Graphical view]
PANTHERPTHR21310. PTHR21310. 1 hit.
PfamPF01636. APH. 1 hit.
[Graphical view]
PIRSFPIRSF000706. Kanamycin_kin. 1 hit.
SUPFAMSSF56112. Kinase_like. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP0A3Y5.

Entry information

Entry nameKKA3_ENTFL
AccessionPrimary (citable) accession number: P0A3Y5
Secondary accession number(s): P00554
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: April 3, 2013
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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