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P0A3Y5

- KKA3_ENTFL

UniProt

P0A3Y5 - KKA3_ENTFL

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Protein
Aminoglycoside 3'-phosphotransferase
Gene
aphA
Organism
Enterococcus faecalis (Streptococcus faecalis)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Resistance to kanamycin and structurally-related aminoglycosides, including amikacin.

Catalytic activityi

ATP + kanamycin = ADP + kanamycin 3'-phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei190 – 1901Proton acceptor By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. kanamycin kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. response to antibiotic Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Antibiotic resistance

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

SABIO-RKP0A3Y5.

Names & Taxonomyi

Protein namesi
Recommended name:
Aminoglycoside 3'-phosphotransferase (EC:2.7.1.95)
Alternative name(s):
APH(3')III
Kanamycin kinase, type III
Neomycin-kanamycin phosphotransferase type III
Gene namesi
Name:aphA
Encoded oniPlasmid pJH10 Publication
OrganismiEnterococcus faecalis (Streptococcus faecalis)
Taxonomic identifieri1351 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesEnterococcaceaeEnterococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 264264Aminoglycoside 3'-phosphotransferase
PRO_0000204805Add
BLAST

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi8 – 147
Beta strandi17 – 215
Beta strandi27 – 359
Beta strandi40 – 467
Helixi48 – 503
Helixi57 – 6711
Turni68 – 703
Beta strandi76 – 827
Beta strandi85 – 917
Beta strandi94 – 974
Helixi98 – 1014
Turni104 – 1063
Helixi108 – 12417
Helixi136 – 14813
Helixi156 – 1594
Beta strandi164 – 1674
Helixi168 – 17710
Beta strandi184 – 1874
Beta strandi195 – 1995
Beta strandi202 – 2065
Beta strandi213 – 2164
Helixi218 – 23114
Helixi236 – 24510
Helixi251 – 26010
Helixi261 – 2633

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1J7IX-ray3.20A1-264[»]
1J7LX-ray2.20A/B1-264[»]
1J7UX-ray2.40A/B1-264[»]
1L8TX-ray2.40A2-264[»]
2B0QX-ray2.70A2-264[»]
2BKKX-ray2.15A/C1-264[»]
3Q2JX-ray2.15A/B1-264[»]
3TM0X-ray2.10A2-264[»]
ProteinModelPortaliP0A3Y5.
SMRiP0A3Y5. Positions 2-264.

Miscellaneous databases

EvolutionaryTraceiP0A3Y5.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

InterProiIPR002575. Aminoglycoside_PTrfase.
IPR024165. Kan/Strep_kinase.
IPR011009. Kinase-like_dom.
[Graphical view]
PANTHERiPTHR21310. PTHR21310. 1 hit.
PfamiPF01636. APH. 1 hit.
[Graphical view]
PIRSFiPIRSF000706. Kanamycin_kin. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.

Sequencei

Sequence statusi: Complete.

P0A3Y5-1 [UniParc]FASTAAdd to Basket

« Hide

MAKMRISPEL KKLIEKYRCV KDTEGMSPAK VYKLVGENEN LYLKMTDSRY    50
KGTTYDVERE KDMMLWLEGK LPVPKVLHFE RHDGWSNLLM SEADGVLCSE 100
EYEDEQSPEK IIELYAECIR LFHSIDISDC PYTNSLDSRL AELDYLLNND 150
LADVDCENWE EDTPFKDPRE LYDFLKTEKP EEELVFSHGD LGDSNIFVKD 200
GKVSGFIDLG RSGRADKWYD IAFCVRSIRE DIGEEQYVEL FFDLLGIKPD 250
WEKIKYYILL DELF 264
Length:264
Mass (Da):30,974
Last modified:July 21, 1986 - v1
Checksum:i7EEA5851D3380C5A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
V01547 Genomic DNA. Translation: CAA24789.1.
X99398 Genomic DNA. Translation: CAA67773.1.
PIRiA00665. PKSOJF.
RefSeqiYP_783930.1. NC_008445.1.

Genome annotation databases

GeneIDi4362702.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
V01547 Genomic DNA. Translation: CAA24789.1 .
X99398 Genomic DNA. Translation: CAA67773.1 .
PIRi A00665. PKSOJF.
RefSeqi YP_783930.1. NC_008445.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1J7I X-ray 3.20 A 1-264 [» ]
1J7L X-ray 2.20 A/B 1-264 [» ]
1J7U X-ray 2.40 A/B 1-264 [» ]
1L8T X-ray 2.40 A 2-264 [» ]
2B0Q X-ray 2.70 A 2-264 [» ]
2BKK X-ray 2.15 A/C 1-264 [» ]
3Q2J X-ray 2.15 A/B 1-264 [» ]
3TM0 X-ray 2.10 A 2-264 [» ]
ProteinModelPortali P0A3Y5.
SMRi P0A3Y5. Positions 2-264.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 4362702.

Enzyme and pathway databases

SABIO-RK P0A3Y5.

Miscellaneous databases

EvolutionaryTracei P0A3Y5.

Family and domain databases

InterProi IPR002575. Aminoglycoside_PTrfase.
IPR024165. Kan/Strep_kinase.
IPR011009. Kinase-like_dom.
[Graphical view ]
PANTHERi PTHR21310. PTHR21310. 1 hit.
Pfami PF01636. APH. 1 hit.
[Graphical view ]
PIRSFi PIRSF000706. Kanamycin_kin. 1 hit.
SUPFAMi SSF56112. SSF56112. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence of the Streptococcus faecalis plasmid gene encoding the 3'5'-aminoglycoside phosphotransferase type III."
    Trieu-Cuot P., Courvalin P.
    Gene 23:331-341(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiKKA3_ENTFL
AccessioniPrimary (citable) accession number: P0A3Y5
Secondary accession number(s): P00554
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: May 14, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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